Modfit安装及分析教程

1、1、安装 Mod f it LT 4、1 软件 修改电脑时间至N年以后，再安装试用版可以实现长时间试用。 2、打开软件 选择试用 Program Information ModFit LT for Windows fersion:4.1.7 Build date:Aug 22 20 K Security mode: Software lode Serial number; Not Registered 30 day trial will expire on 08/01/2080 x only Not for use in therapeutic and dagix)stc procedures

2、. Copyright 1994-2014 Verib/ Software Hsse. All rights reserved. Credits. Mod Fit U and- logos are trademarks of Verity Software House. Con tact In formation Verity Software House Web site:www vsh com Email:verity vsh. com Phone:207.729.6767 Registratjon. 3、打开数据，选择FCS文件 4.激活Gate 1,选择D NA染料得H/A图形 Ana

3、lysis Setup Gate 1 on one of the tems Name Gate 1 ，Gate 2 Gate 3 Analysis Popula.- CM All 20004900 FSC-H （X1000） OK 5、在坐标轴附件右键,修改坐标参数，线性，上限范围（根据实际情况调 整范围） 000 L ooolrx) UU9 H,0 辱CDd 亠 Edit X Parameter;. DO Copy to Clipboard 曰 Edit Properties. 0 Edit Y Parameter. in 20004-00060008000 FL9-AHoechst 333

4、42 PB450-A- Edit Properties for FL9-A Hoechst 33342 PB450-A Name Value 3 FCS Keyword Name($PnNjPnS) FL9-A Hoechst 33342 PB450-A 自 Parameter Resolution10000000.00 Alias and Transform Parameter Alias Transform Parameter Auto-zoom Zoom Type Fixed Upper Boundary Percentile FL9-A Hoechst 33342 PB4-50-A L

5、inear 1000000.00 99.00 Select the type of transform to use for this parameter. 6.画门排除黏连体 Gate 1 200040006000SOOO FL9-AHoechst 33342 PB450-A(x100) 000 L (000 L X】 008 009 0C3寸 oom 工osngd cnlpao 工工，61ljl 7、选择检测直方图得参数检查范围就是否合适，然后点击OK Analysis Setup Click on one of the tems bdew to edit. Analysis Popula

6、tion 8、 使用Au t o An a lyze预分析，如果结果与预期不符可记下一 些参数如“G2/G1”得值。 Cebris J Acqrcofltcs CipG! 200040006000 FL9-A Hoechst 33342 PB460-A(x 100) File analyzed: Hela R7.fcs Dat analyzed 2Jul-2015 Model 2DA0n_DSn_TSD Analysis 1y丙 Automatic analysis Auto Unearity: No Ploidy Mede: First cycle is diploid Diploid. 8

7、8.54 % DipG1 73 34 % at 313982 09 Dip G2 8 00 % 衣 590714 竺 DipS: 18.66 % |G2H.88| %CV: 4.91 Tetraploid: 11.46 % An1 G1. 100.00 % at 590714.54 An1 G2 % al An1S % G2/G1 %CV: 3.01 DI: 1.88 Total Areuploid S-Phaso: % Total S-Phase: 16.62 % Total BAD 1 60 % Debris 0 31 % Aggregates: 2.49 % Modeled events

8、: 9988 All cyclo o/onts: 9708 Cycle events per channel: 120 RCS 2 139 Edit Properties for Manual Analysis NameDescript on Manual AnclysM Settings to manuolly ono CydelCell cycle Name Auto Debris Auto Aggregates Linearity 1.88 Internal Standard!； 0 Number of Cycles 1 Model Templatei , Range Positions

9、 / Ccmpuw ran并 pcsfMns 7 On Z On 匚 DoKt Mod Description Auto Debris Compensation Auto Aggregate Compensati Check to model apoptosis p G2/G1 ratio (1.50 to 2.50) (1. Number of internal standard Number of cell cycles in sam Select a modd template Choose an opton 9、 使用Choo s e Model手动分析,主要修改线性度与周期个数，点

10、击 OK。可关闭Auto Debri s 与 Auto Aggreg a tes hi njfrber of cdl cydes h sample (lto 4 10、可根据需要移动各期得门 os 009 00b ooe aqlunN ip G1 Dip Gl: Left: 290196 Right: 329412 Mean: 311899 SD: Debris 2000 4000 6000 FL9-A Hoechst 33342 PB450-A (x100) 11 点击F it完成分析 -二二一二二-二二-二二-二二一 一二一-一 osS9 00S 00 00C 0500- JaqEnN n

11、 opgi OpG2 S DipS 200040006000 FL9-A Hoechst 33342 PB450-A(x 100) R1 20004000 6000 8000 File analyzed: Hela R7 fcs Date analyzed 2-Jul-2015 Model 1nn0n_DSD Analysis type: Manual onalysis Auto Linearity: No Reidy Mode: First cycle is diploid Diploid 100 00 % Dip G1: 64.76 % at 314492.34 Dip G2: 22.49 % at 591245.60 Dip S. 12.75 % G2/G1. 1 66 %CV 4 84 Total S-Phase: 12.75 %