常见载体引物表及序列

1、备注：rv-m=m13r-48，m13-47=m13f-47，我们将说法修正的专业一些。如果对本表中的引物有疑问，请跟纪义芳核实。 vectorvectorprimer(f)primer(f)primer(r)primer(r) paav-mcsf（-globin）r（hghpolya） pactt7t3 pact25ad（同gal4ad） 3ad/pgal4-adrv（pgal4- adrv离mcs少于20bp，优先用 3ad） pas2-15bd(同gal4bd) 3bd/pgal4- bdrv（优先用3bd） pb42adpb42adfpb42adr pbacpak8bac1/ph-pr

2、ofor/baculovfor bac2/baculovrev pbg-t(天根）t7t3 pbi12135s无反向引物 pbk-cmvt7/m13ft3/m13r pbluescript ii sk (+/- )/pbluescript ii ks (+/- )/pbluescript ks (+/- )/pbluescript sk (+/-)t3/m13rm13f/t7 pbudce4.1cmv-profor/t7c-mycrev/ebvrev pbv220pbv220fpbv220r pcambia1301（1300）p1p2 pcambia2300rv-m/m13r m13-47/m

3、13f(rv-m/m13-47距 离mcs更远) pcantab_5e pcantab5-s1/pcantab5- s3/pcantab5-r1 pcantab5-s6/pcantab5- r2/pcantab5-s4 pcat3-enhancerrvp3无反向引物 pcdna3.0cmv-f/t7sp6/bgh pcdna3.1t7bgh pcdna4cmv-f/t7bgh pcdna5cmv-f/t7bgh pcdna6 cmv-f/t7(优先用t7，cmv-f 离mcs约200bp)bgh pcdnaiit7sp6 pce2.1m13fm13r pcep4pcep-febvrev pcf

4、-tm13fm13r pci（neo） 17base/t7（17base在t7之前 ） pci-neof(暂时不要用)ebvrev pcms-egfpt7t3 pcmv-3tag-4at3/pflag-cmv-ft7 pcmv5pcmv5f无反向引物 pcmv6/pcmv6-xl4(5,6)/pcmv-ac v1.5xl39 pcmv-mcsf（-globin）r（hghpolya） pcmv-myc/hapcmv-fpcmv-r pcmv-sportm13f/t7sp6/m13r pcmv-tag(kan+)t3t7 pcoldi/pcoldiiipcoldi-fpcoldi-r pcr2.

5、1-topom13rm13f/t7（t7离mcs只有6bp） pcr3.1t7bgh pcs2sp6t7 pcs2+ (mcsi)sp6ebvrev pcs2+ (mcsii)t3psg5rv pdbleupdbleu-fpdbleu-r pdnr-libm13fm13r pdonarm13fm13r pdonr201seql-aseql-b pdonr221m13fm13r pdrivet7sp6 pdsred1-c1/pdsred2-c1pdsred-ex-c1-f pdsred1-n1cmv-proforrfp-nrev peasy-bluntm13rm13f/t7 peasy-e1t7

6、t7ter(离插入位点不到20bp） peasy-e2t7t7ter peasy-t1/peasy-t1 simplem13r/rv-mm13f/m13-47(优先用rv-m和m13-47） peasy-t3m13f/t7m13r/sp6(优先用m13引物) pecfp-c1egfp-cforsv40-parev pecfp-n1cmv-proforpegfp-n-3/egfp-nrev pef/myc/erpef-fpcdna3.1r pegfp（5mcs）m13r(距第一个酶切位点29bp）pegfp-n-3/egfp-nrev pegfp（3mcs）pegfp-c-5无反向引物 pegf

7、p-f/c1/c2/c3egfp-cforsv40-parev pegfp-n(kan+)pegfp-n-5pegfp-n-3 pentr/d (-topo)(kan+)m13f/seql-am13r/seql-b pet-*(his)(kan+)t7t7ter pet22a(b,c)(amp+)t7t7ter pet24(28,30,49)(kan+)t7t7ter pet32a(b,c)(amp+)s.tagt7ter pet-43.1a (b, c)(+)s.tag无反向引物 pet44(amp+)t7/s.tagt7ter pet50(amp+)t7/s.tagt7ter petdue

8、t-1(mcsi)pbrrevbamduetdown1 petduet-1(mcsii)duetup2t7ter peyfp-c1pegfp-c-5/egfp-cforpegfp-c-3/sv40-parev peyfp-n1pegfp-n-5/cmv-proforpegfp-n-3/egfp-nrev pfastbacph-profor/baculovforpfastbac-rev pfastbac dual (mcsi)baculovfor/ph-proforpfastbac-rev/sv40-parev pfastbac dual (mcsii)p10-proforhsvtk-parev

9、 pfastbac ht_a (_b, _c)pfastbac-fw/ph-proforpfastbac-rev pfastbac1ph-profor/baculovforpfastbac-rev pfastbac1-gus无正向引物sv40-parev pfastbacht-cat无正向引物sv40-parev pflag-ats/macn26forptrchis-rv（同pbadrv） pflag-cmv1pflag-cmv-f/cmv-30pflag-cmv-r/cmv-24 pflag-cmv2cmv30cmv24 pgad424gal4ad(5ad)3ad/pgal4-adrv pg

10、adghgal4ad(5ad)3ad pgadt75ad/t7pgal4-adrv pgadt7-rect73ad pgapza_a (_b, _c)a-factor/gap-dh-profor3aox/c-mycrev pgbkt7(kan+)t7/gal4-bd-cfor3bd/pgal4-bdrv pgem-t(easy)m13f/t7sp6/m13r pgex-2t/pgex-2tk/pgex-3xpgexfw(同pgex-f)pgexrv(同pgex-r) pgex-4t-1(2，3）pgexfw(同pgex-f)pgexrv(同pgex-r) pgex-5x-1(2,3）pgexf

11、w(同pgex-f)pgexrv(同pgex-r) pgex-6p-1(2，3）pgexfw(同pgex-f)pgexrv(同pgex-r) pgex-kgpgexfw(同pgex-f)pgexrv(同pgex-r) pgl2glp1glp2 pgl3-basic (mcs i)rvprimer3glprimer2 pgl3-basic (mcs ii)rvprimer4无反向引物 pgl3-control (mcs i)rvprimer3glprimer2 pgl3-control (mcs ii)rvprimer4plxsnrv pgl3-enhancer (mcs i)rvprimer3

12、glprimer2 pgl3-enhancer (mcs ii)rvprimer4plxsnrv pgl3-promoter (mcs i)rvprimer3glprimer2 pgl3-promoter (mcs ii)rvprimer4无反向引物 pgs21at7t7ter pinpointtmpinpoint primersp6 pires (mcs i)t7 piresrv pires (mcs ii)无正向引物t3/ebvrev pires2-egfppires2-egfp.p5/cmv-profor pires2-egfp.p3/ires-r pires-hrgfp-2acmv-p

13、rofor/ t3pires-hrgfprv pkk223/pkk223-3pkkfw/tac-proforpkkrv/pbadrv plexaplexa-f/lexafor plexa-r/pgal4-bdrv plncxplncx-fplncx-r plncx2cmv-proforprevtrerv plxsnplxsn-f/plxsnfw plxsn-r/pmscvrev pmal-c2e/p2xmalem13-47 pmal-c2xpmal-c2x-fpmal-c2x-r pmd18-trv-m m13-47 pmd19-tm13-47rv-m potb7m13f/t7m13r/sp6

14、 ppc86ppc86-fppc86-r ppcr-scriptt3t7 ppic9k(amp+、zeo+）5aox/a-factor3aox ppicz_a (_b,_c)5aox3aox pproexhtatac-profor/rv-mpbadrv/ptrchis-rv pqe30/40/80lpqe30-fpqe30-r prk5sp6psg5rv prsett7/xpressfort7ter prsfduet-1(mcsi)duetup1duetdown1 prsfduet-1(mcsii)duetup2t7ter pshuttle-cmvpshuttle-cmv-fpshuttle-

15、cmv-r psilencer3.1-h1hygrom13-473.0rev psilencer4.1-cmvpsilencer4.1-fpsilencer4.1-r psilencer2.0-u6t7 2.0rev/m13f(m13f比2.0rev 离mcs近30bp左右) psk-cmv(kan+)t7t3 psp64/65sp6m13r psp72sp6t7 psport1sp6/m13ft7/m13r pstblue-1t7sp6 psupert7t3 pt7blue(r)t7m13-47 pta(天为时代)t7t3 pta2m13f/t7(优先使用m13f）t3/m13r pt-ad

16、vm13rm13f/t7 ptargettmptarget.f(在t7上游)/t7ptarget.r pthiohis a( b, c)trxforwardtrxreverse pto-t7t7t7ter ptrc99a-cptrc99c-fptrc99c-r ptriex1.1_neot7ires-r ptriplex2triplex-ld-5t7/triplex-ldrv puc18(19)/118(119)/puc57-trv-m/m13rm13-47/m13f puc20m13fm13r pucm-tm13f/t7m13r pvax1t7/ cmv-proforbgh pwpxlef-

17、la无反向引物 pwsk29t7/m13f/m13-47t3/m13r/rv-m pxt7t7sp6 pyes2t7pyes2.r psilencer_4.1-cmv_neopsilencer-4.1-fpsilencer-4.1-r pacycduet-1（mcs）acycduetup1duetdown1 pacycduet-1（mcs）duetup2t7ter prk5-flagsp6psg5rv topo-pentr-sdm13fm13r pcmv-tag-2bt3t7 pcmv-tag-3bt3t7 pgl4.10rvprimer3glprimer4 pmirglo pjet1.2/b

18、luntpjet1.2-fpjet1.2-r pegfp-n3cmv-proforegfp-nrev pearlygate20135s pearlygate10235s pbabe-puropbabe5pbabe3 ppzp111m13r/rv-mm13f/m13-47 ppzp201m13r/rv-mm13f/m13-47 pbindt7/gal4bd/cmv-f/sv40/pgexrv ebvrev/pbabe3 p3xflag cmv14cmv30cmv24 引引物物名名称称序序列列(5-3)(5-3) 1492rggttaccttgttacgactt 27fagagtttgatcctg

19、gctca与8f序列一致 3.0revgagttagctcactcattaggc 35sgacgcacaatcccactatcc 3adagatggtgcacgatgcacag 3aoxggcaaatggcattctgacat 3bdtaagagtcactttaaaatttgtatac 5ad（同gal4ad）taccactacaatggatgatg 5aoxgactggttccaattgacaagc 96giii（同m13-96）ccctcatagttagcgtaacgm13噬菌体的通用引物 a-factortactattgccagcattgctgc bac1aaccatctcgcaaata

20、aata bac2acgcacagaatctagcgctt baculovfortgttttcgtaacagttttg baculovrevaacgcacagaatctagcgc bghtagaaggcacagtcgagg cmv-24ttaggacaaggctggtgg cmv-30ataaccccgccccgttg cmv-proforatgggcggtaggcgtg cmv-rtcgttgggcggtcagc c-mycrevctcttctgagatgagtttttg duetdown1gattatgcggccgtgtacaa duetup1gatctcgacgctctccct duet

21、up2ttgtacacggccgcataatc ebvrevgtggtttgtccaaactcatc ef-1aforwardtcaagcctcagacagtggttc egfp-nrevcgtcgccgtccagctc egfp-cforagcacccagtccgccctgagc f（-globin）attctgagtccaagctaggc gal4bdtcatcggaagagagtag gap-dh-proforgtccctatttcaatcaattgaa glp1tgtatcttatggtactgtaactg glp2ctttatgtttttggcgtcttcca gpdpromoter

22、-fcggtaggtattgattgtaattctg ires-rcctcacattgccaaaagacg its1tccgtaggtgaacctgcgg its3gcatcgatgaagaacgcagc its4tcctccgcttattgatatgc its5ggaagtaaaagtcgtaacaagg lexaforcgtcagcagagcttcaccattg m13-47cgccagggttttcccagtcacgac m13rcaggaaacagctatgacc rv-magcggataacaatttcacacagga与m13_puc_rev_primer序列相同 maleggtcg

23、tcagactgtcgatgaagcc mu6f2（mouse）tagtatccgacgccgccatc n26forcatcataacggttctggc nosf-primercacctgatcccagcttgc nosr-primercgtccagctccatgttgc p1ccaggctttacactttatgc p2gcgattaagttgggtaacgc p12584ttttcagtatctacgat p17110taccactacaatggatg p10-proforcggacctttaattcaaccc pacgp67rvttggttcttgccgggtc pas2-1ftcat

24、cggaagcgagtag pas2-1rcgttttaaaacctaagagtcac pb42adfccagcctcttgctgagtggagatg pb42adraagccgacaaccttgattggag pbabe3accctaactgacacacattcc pbabe5ctttatccagccctcac pbadfwccatagcatttttatccataag pbadrvatctgtatcaggctgaaaatc pbrrevbamggtgatgtcggcgatatagg pbv220faagaagggcagcattcaaag pbv220rctgcgttctgatttaatctg

25、 pcantab5-r1ccatgattacgccaagctttggag pcantab5-r2cgatctaaagttttgtcgtctttc pcantab5-s3ggttcaggcggaggtggctctgg pcantab5-s4ccagagccacctccgcctgaacc pcdna3.0fctctggctaactagagaacc pcdna3.0rggcaactagaaggcacagtc pcdna3.1fctagagaacccactgcttac pcep-fagagctcgtttagtgaaccg pcmv-5rattatagaggacacctagtc pcmv5fttc ca

26、a aat gtc gta ata ac pcmv-ftctaaaagctgcggaattgt pcmv-rtccaaactcatcaatgtatc pcoldi-facgccatatcgccgaaagg pcoldi-rggcagggatcttagattctg pdbleu-fgaataagtgcgacatcatcatc pdbleu-rgtaaatttctggcaaggtagac pdsred-ex-c1-ftcccacaacgaggactacac pdsred-n1-rtgaagcgcatgaactccttg pegfp-c-3tatggctgattatgatcagt pegfp-c-5

27、catggtcctgctggagttcgtg pegfp-n-5tgggaggtctatataagcagag pfastbac-ftgaagtggttcgcatcctc pfastbac-rtggacaaaccacaactagaatg pfastbac-revcctctacaaatgtggtatgg hsvtk-parevgtctccttccgtgtttcag pfastbac-fwgtccgaaaccatgtcgtac pflag-cmv-fggtaggcgtgtacggtgg pflag-cmv-rgcactggagtggcaactt pgal4-adrvgaacttgcggggttttt

28、c pgal4-bdrvaatcataagaaattcgccc pgexfwggcaagccacgtttggtg pgexrvgagctgcatgtgtcagagg ph-proforaaatgataaccatctcgc pinpointprimercgtgacgcggtgcagggcg pires2-egfp.p3aacgcacaccggccttattc pires2-egfp.p5gtaggcgtgtacggtgggag piresrvgccctagatgcatgctcg pires-hrgfprv gtccttatcatcgtcgtctt pkkfwagcggataacaatttcac

29、pkkrvtctctcatccgccaaaacag plxsnfwcccttgaacctcctcgttcg plxsnrvactttccacaccctaactg plxsn-fcttgaacctcctcgttcgac plxsn-rgttgctgactaattgagatg plncx-fagctcgtttagtgaaccgtcagatcg plncx-racctacaggtggggtctttcattccc plexa-fcgtcagcagagcttcaccat plexa-rtaaaacctaagagtcacttt pmal-c2x-ftgcgtactgcggtgatcaac pmal-c2x

30、-rctgcaaggcgattaagttgg ppc86-ftataacgcgtttggaatcact pqe30-ftgagcggataacaatttcac pqe30-rgttctgaggtcattactgg prevtrervccccctttttctggagac psg5rvgaaatttgtgatgctattgc pshuttle-cmv-fggtctatataagcagaggtg pshuttle-cmv-rgtggtatggctgattatgatcag psilencer4.1-rcggtaggcgtgtacggtg ptarget.fccaggattttcccagtcac pta

31、rget.rggctttacactttatgcttc ptarget-seqttacgccaagttatttaggtgaca ptrc99c-fttgcgccgacatcataac ptrchis-forwardgaggtatatattaatgtatcg ptrchisa-fcatggtatggctagcatgac ptrchisa-rcaggctgaaaatcttctctc pyes2.r/cyc1-terminatorgcgtgaatgtaagcgtgac rfp-nrevgttcacggtgccctcc r（hghpolya）tagaaggacacctagtcaga rvp3ctagca

32、aaataggctgtccc rvp4gacgatagtcatgccccgcg pcantab5-s1caacgtgaaaaaattattattcgc pcantab5-s6gtaaatgaattttctgtatgagg seql-agcagttccctactctcgc seql-bcatcagagattttgagacac sp6atttaggtgacactatag s.tagcgaacgccagcacatggaca sv40ggaggcttttttggaggc t3attaaccctcactaaaggga t7taatacgactcactatagg t7（17base）acatccacttt

33、gcctttctc t7tertgctagttattgctcagcgg tac-proforaattaatcatccggctcg trxforwardttcctcgacgctaacctg trxreversetgtaaaacgacggccagtgc triplex-ldrvatacgactcactatagggcgaattggc triplex-ld-5ctcgggaagcgcgccattgtgttgg u6-promoter（human）ccgtaacttgaaagtatttcg v1.5ggactttccaaaatgtcg v5revatcgagaccgaggagagg wpre-rcatagcgtaaaaggagcaaca