2000分子生物改版试卷B.doc

2000级医学检验专业分子诊断学考试试卷(B卷)_A1型题（最佳选择题肯定型）每一道题有A,B,C,D,E五个备选答案，问题表述形式为肯定陈述，在答题时只需从5个备选答案中选择一个最合适的作为正确答案，并在答卷上将相应题号的相应字母画一条黑粗线。_1.What is the major characteristics of neurofibromatosis 2 ? A. caf an lait spots B. cutaneous or subcutaneous neurofibromasC. axillary freckling D. acoustic neuromas and meningiomasE. scoliosis2.Which is required to activate LPL?A. apoA B. apoC C. apoC D. apoB48 E. apoB1003.Which apo is CM essential?A. apoA B. apoA C. apoB48 D. apoB100 E.apoC4.In the procedure of Genomic DNA isolation, which of the following reagent serves to dissolve the cell membrane and denatures proteins?A. cold phosphate-buffered saline B. sodium dodecylsufate C. phenol saturated with 0.5M Tris-HCl D. dialysis buffer E. chloroform5. To evaluate if a neonate has infected HIV, which of the following methods are usually be used?A. ELISA B. agglutilation C. PCR D. Wester-blot E. immunofluorescence staining6. Which RNA can be transferred into mRNA? A. hnRNA B. RNAi C. scRNA D. sRNA E. snRNA7. DNA fragment and DNA probes are to southern blotting as ( ) are to Northern blotting .A. DNA fragments and RNA probes B. RNA fragments and DNA probesC.RNA fragments and RNA probes D. protein fragments and RNA probesE. protein fragments and DNA probes8.Which is the smallest densist lipoproteins?A.CM B.VLDL C.LDL D.HDL E.IDL9.Transfer of nucleic acids by vacunm blotting is more efficient than capillary transfer and has been reported to result in a ( ) increase in final hybridization singal obtained.A. one-to tow fold B. tow-to three fold C. one-to three fold D. three-to four fole E. tow-to four fold10.What disorder is spinal muscular atrophy?A.centralnervoussystemdisorder B. autosomal-recessive disorderC.endocrinesystemdisorder D.autosomal-dominant disorderE.immune system disorder11 What is the major role of EDTA in the basic nucleic acid procedures?A. providing a proper condition of salt for DNA to dissolve B. denaturing proteins in the solution C. chelating divalent cations required for nucleasesD. buffering the solution pHE. preventing microorganism contamination12. If the absorbance at 260nm for a DNA sample is 0.3 O.D, which of the following item correspondent to the concentration of the sample? A. 6.9g/ml B. 9.9g/ml C. 11.0g/ml D. 12.0g/ml E. 15.0g/ml13. The base existing only in RNA is A. adenine B. guanine C. thymine D. cytosine E. uracil_A2型题（最佳选择题否定型）每一道题有A,B,C,D,E五个备选答案，问题表述形式为否定陈述，在答题时只需从5个备选答案中选择一个最合适的作为正确答案，并在答卷上将相应题号的相应字母画一条黑粗线。_14.Which of the clinical feature belowed is not the clinical characteristic of spial muscular atrophy?A.anteriorhorncellsdegenerate B.hypotoniaC.symmetricalmuscleweakness D.wasting of voluntary muscle E.reduced fertility 15 Which of the following is NOT correct about application of the Southern Blot? A. Its useful in detecting gene amplification B. Its useful in detecting gene mutationC. Its useful in detecting gene deletionD. Its useful in detecting gene rearrangementE. Its useful in detecting gene total RNA16. Which of following is not correct about factors influencing hybridization?A. salt concentration B. solvent concentration C. temperature D. relative strength of nucleic acid hybrids E. background signal_填空题（每空1分。共20分）_17.The usually used molecular techniques un laboratory diagnosis of infection diseases include , and .18.Key enzyme of lipoprotein catabolize have ， ， .19.The mRNA is copied beginning from its -end.20.DNA amplification cycle have three steps: denaturation, and .21. and probes must be visualized by exposing the hybridized membrane to X-ray film , producing an autoradiograph.22.Rate of capillary transfer is dependent on the of the fragments.23.For FRAXA ,the trimucleotide repeat is localized to the region. For SBMA,HD,DRPLA,on the other hand, the repeat is within the region,and for DM, the repeat is present in the region.24. The relative strength of nucleic acid hybrids is .25.Poly(A)-mRNA can be isolated from total RNA by -selection.26.Taq polymerase works best at Centigrade._名词解释（每题4分，共24分）_27.Souther n Blot28.Capillary transfer29.Taq polymerase30.allele31.neurofibromin32.lipoproteins_E1型题（问答题，每题8分，共40分）将答案写在答题纸上，并表明题号。_33. What is ISH? Please illustrate its advantage and disadvantage.34. Please simply interpret the advantages o