论文资料-维生素C论文：维生素C纳米脂质体的制备及其性质的研究.doc

莂芇袂螃肂蒂螈袂膄芅蚄袁芆蒁薀袀肆芃薆衿膈蕿袄衿芁莂螀袈莃薇蚆袇肃莀薂羆膅薅蒈羅芇莈螇羄羇薄蚃羄腿莆虿羃节蚂薅羂莄蒅袃羁肄芈蝿羀膆蒃蚅聿芈芆薁肈羈蒁蒇肈肀芄袆肇节蒀螂肆莅莂蚈肅肄薈薄肄膇莁袃肃艿薆蝿膂莁荿蚅膂肁薅薁螈膃莇蒇螇莆薃袅螆肅蒆螁螅膈蚁蚇螅芀蒄薃螄莂芇袂螃肂蒂螈袂膄芅蚄袁芆蒁薀袀肆芃薆衿膈蕿袄衿芁莂螀袈莃薇蚆袇肃莀薂羆膅薅蒈羅芇莈螇羄羇薄蚃羄腿莆虿羃节蚂薅羂莄蒅袃羁肄芈蝿羀膆蒃蚅聿芈芆薁肈羈蒁蒇肈肀芄袆肇节蒀螂肆莅莂蚈肅肄薈薄肄膇莁袃肃艿薆蝿膂莁荿蚅膂肁薅薁螈膃莇蒇螇莆薃袅螆肅蒆螁螅膈蚁蚇螅芀蒄薃螄莂芇袂螃肂蒂螈袂膄芅蚄袁芆蒁薀袀肆芃薆衿膈蕿袄衿芁莂螀袈莃薇蚆袇肃莀薂羆膅薅蒈羅芇莈螇羄羇薄蚃羄腿莆虿羃节蚂薅羂莄蒅袃羁肄芈蝿羀膆蒃蚅聿芈芆薁肈羈蒁蒇肈肀芄袆肇节蒀螂肆莅莂蚈肅肄薈薄肄膇莁袃肃艿薆蝿膂莁荿蚅膂肁薅薁螈膃莇蒇螇莆薃袅螆肅蒆螁螅膈蚁蚇螅芀蒄薃螄莂芇袂螃肂蒂螈袂膄芅蚄袁芆蒁薀袀肆芃薆衿膈蕿袄衿芁莂螀袈莃薇蚆袇肃莀薂羆膅薅蒈羅芇莈螇羄羇薄蚃羄腿莆虿羃节蚂薅羂莄蒅袃羁肄芈蝿羀膆蒃蚅聿芈芆薁肈羈蒁蒇肈肀芄袆肇节蒀螂肆莅莂蚈肅肄薈薄肄膇莁袃肃艿薆蝿膂莁荿蚅膂肁薅薁螈膃莇蒇螇莆薃袅 维生素C论文：维生素C纳米脂质体的制备及其性质的研究【中文摘要】维生素C是一种水溶性维生素,具有美白祛斑、延缓衰老的美容功效,但是容易氧化变质,且不易被皮肤吸收。脂质体作为一种新兴药物载体,对包封的药物具有保护作用,从而提高其作用时间。本课题以水溶性物质维生素C为模型材料,采用动态高压微射流(DHPM)作为核心技术,结合传统脂质体制备方法,通过工艺优化,制备了维生素C脂质体和维生素C纳米脂质体,并研究了这两种脂质体的物理化学性质(粒径、包封率、外观形貌、稳定性、缓释性)和生物利用率(抗氧化能力和皮肤渗透性)。本课题建立了测定维生素C脂质体包封率的分析方法：采用透析法分离游离维生素C、甲醇破乳、紫外分光光度法测定包封率。制备了维生素C脂质体和维生素C纳米脂质体,考察了工艺配方因素的影响。结果表明,维生素C脂质体的最优制备条件为：制备温度为65,维生素C浓度为2 mg/ml,维生素C和总脂材质量比为1：25,卵磷脂和胆固醇质量比为5：1,表面活性剂和总脂材质量比为2：10,卵磷脂和无水乙醇质量体积比为2.5：100。制备维生素C纳米脂质体的最优条件为：制备温度60,维生素C浓度5 mg/ml,维生素C和总脂材质量比为1：10,卵磷脂和胆固醇质量比为4：1,表面活性剂和总脂材质量比为4：10,卵磷脂：无水乙醇=10：100,处理压力为140 MPa,处理2次。对维生素C脂质体和维生素C纳米脂质体的理化性质、缓释性和稳定性进行评价,结果表明：维生素C纳米脂质体较好,包封率为(47.166.28)%,平均粒度为(73.94.4)nm,微观形貌呈实体球状,贮藏稳定性、体内稳定性良好,缓释作用明显。对比研究了维生素C脂质体和维生素C纳米脂质体的抗氧化功能和皮肤穿透能力,结果表明,两种脂质体的维生素C抗氧化功能和游离维生素C基本一致；皮肤渗透能力的排列顺序为：维生素C纳米脂质体维生素C脂质体游离维生素C。【英文摘要】Vitamin C is water soluble,it has the effect of skin lightening and anti-aging,but it is easy to oxidative deterioration, and not easily penetrate through the skin. As a new drug carrier,liposomes has the effect of protecting the core material encapsulated and improving the bioavailability of drugs.As the core material, Vitamin C was prepared to Vitamin C liposomes and Vitam in C nanoliposomes using dynamic high-pressure microfluidization (DHPM) as a core technology which combined with the traditional method of liposomal preparation. In addition, both the physical and chemical properties of liposomes (mean particle size, encapsulation efficiency, morphology, stability, time-controlled releasing,) and bioavailability (antioxidant capacity and skin permeability.) were characterized.The analysis method of determinating entrapment efficiency of vitamin C liposomes was established:separated free vitamin C by dialysis, breaken the liposomes by methanol emulsion, determinated Vitamin C content by UV spectrophotometry.The traditional liposomal preparation methods and DHPM combined with the traditional method of liposomal preparation were developed to prepare vitamin C liposomes and vitamin C nanoliposomes, respectively. Each factor was analyzed and the orthogonal test was taken to optimize the formula.The optimized formula of vitamin C liposomes as follows:the preparation temperature was 65,the concentration of vitamin C was 2 mg/ml, the mass ratio of vitamin C and lipids was 1:25, the mass ratio of phospholipid and cholesterol was 5:1, the mass ratio of Tween-80 and lipids was 2:10,the mass volume ratio of phospholipid and ethanol was 2.5:100. The optimized formula of vitamin C nanoliposomes as follows:the preparation temperature was 60,the concentration of vitamin C was 5 mg/ml, the mass ratio of vitamin C and lipids was 1:10, the mass ratio of phospholipid and cholesterol was 4:1, the mass ratio of Tween-80 and lipids was 4:10,the mass volume ratio of phospholipid and ethanol was 10:100,the treatment pressure and times were 120 MPa and 4.The physicochemical properties of both vitamin C liposomes and vitamin C nanoliposomes were evaluated.Vitamin C nanoliposomes had advantages over vitamin C liposomes with the encapsulation efficiency(47.166.28%),the mean particle size(73.94.4 nm),showed a solid spherical morphology,good storage and vivo stability,significantly slow release.Compared the antioxidant capacity and the skin penetration of vitamin C liposomes and vitamin C nanoliposomes, results showed that, compared to free vitamin C,the antioxidant capacity of two types of liposomes was not affected; he order of skin penetration was:Vitamin C nanoliposomes Vitamin C liposomes Free Vitamin C.【关键词】维生素C 动态高压微射流 纳米脂质体 稳定性 皮肤渗透【英文关键词】Vtamin C DHPM Nanoliposomes Stability Skin penetration【目录】维生素C纳米脂质体的制备及其性质的研究摘要3-4ABSTRACT4-5第一章 前言9-281.1 脂质体研究现状9-231.1.1 脂质体简介9-111.1.2 脂质体分类11-121.1.3 脂质体的特点12-131.1.4 脂质体的制备方法13-161.1.5 纳米脂质体的概述161.1.6 脂质体理化性质的评价16-171.1.7 脂质体的应用概况17-231.2 维生素C简介23-241.2.1 维生素C的结构及性质231.2.2 维生素C的美容功效23-241.3 动态高压微射流技术简介24-251.3.1 动态高压微射流技术概述241.3.2 动态高压微射流技术国内外研究现状24-251.4 选题意义25-261.5 研究内容及创新点26-28第二章 维生素C含量及脂质体包封率测定方法的建立28-352.1 主要试剂及仪器28-292.2 实验方法29-312.2.1 破乳剂的确定292.2.2 维生素C的测定29-302.2.3 分离方法的筛选30-312.2.4 维生素C脂质体包封率的测定312.3 结果与讨论31-342.3.1 破乳剂的确定31-322.3.2 维生素C的测定322.3.3 透析法测定维生素C脂质体的包封率32-342.4 本章小结34-35第三章 传统方法制备维生素C脂质体的研究35-473.1 主要试剂及仪器35-363.2 实验方法36-383.2.1 制备方法的筛选36-373.2.2 实验设计37-383.3 结果与讨论38-463.3.1 制备方法的筛选38-393.3.2 单因素实验结果39-433.3.3 正交实验结果43-453.3.4 验证实验结果45-463.4 本章小结46-47第四章 动态高压微射流技术制备维生素C纳米脂质体的研究47-614.1 主要试剂及仪器47-484.2 实验方法48-514.2.1 制备方法的筛选48-504.2.2 实验设计50-514.3 结果与讨论51-604.3.1 维生素C纳米脂质体制备方法的筛选51-524.3.2 单因素实验结果52-574.3.3 正交实验结果57-594.3.4 验证实验结果59-604.4 本章小结60-61第五章 维生素C脂质体与维生素C纳米脂质体理化性质及稳定性研究61-725.1 主要试剂及仪器61-625.2 实验方法62-645.2.1 脂质体的制备625.2.2 脂质体平均粒度大小及其分布的测定62-635.2.3 负染法透射电镜形态形貌观察635.2.4 脂质体的稳定性实验63-645.2.5 脂质体的缓释性实验645.3 结果与讨论64-705.3.1 脂质体的平均粒度大小及其分布64-655.3.2 脂质体的TEM形貌结构65-665.3.3 脂质体悬浮液的稳定性66-695.3.4 脂质体的缓释性69-705.4 本章小结70-72第六章 维生素C脂质体与维生素C纳米脂质体生物利用率的研究72-796.1 主要试剂及仪器72-746.2 实验方法74-766.2.1 脂质体的制备746.2.2 溶液的配制74-756.2.3 L-DOPA氧化酶抑制实验756.2.4 DPPH自由基清除实验756.2.5 体外透皮实验75-766.3 结果与讨论76-786.3.1 L-DOPA氧化酶抑制