L-Cystine-hydrochloride-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors • ScreeningLibraries • Proteinswww.MedChemEL-CystinehydrochlorideCat.No.:HY-W009356CASNo.:34760-60-6分子式:C₆H₁₄Cl₂N₂O₄S₂分子量:313.22作用靶点:EndogenousMetabolite;Ferroptosis;ROSKinase;Keap1-Nrf2;ReactiveOxygenSpecies作用通路:MetabolicEnzyme/Protease;Apoptosis;ProteinTyrosineKinase/RTK;NF-κB;Immunology/Inflammation储存方式:RT,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(RT,protectfromlight)溶解性数据体外实验H2O:125mg/mL(399.08mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.1926mL15.9632mL31.9264mL5mM0.6385mL3.1926mL6.3853mL10mM0.3193mL1.5963mL3.1926mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存方式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。BIOLOGICALACTIVITY生物活性L-Cystinehydrochloride是具有口服活性的L-Cysteine(HY-Y0337)的细胞外形式，存在于植物和动物的蛋白质中。L-Cystinehydrochloride升高Nrf2蛋白表达含量并激活Nrf2转录因子。L-Cystinehydrochloride减少了ROS的产生，并保护细胞免受氧化剂或Doxorubicin(HY-15142A)诱导的凋亡(apoptosis)。L-Cystinehydrochloride与L-theanine(HY-15121)联合使用通过提高谷胱甘肽(GSH)水平和T辅助细胞2(Th2)介导的反应，增强了抗原特异性IgG的产生。L-Cystinehydrochloride有望用于囊性尿症和肾结石的研究[1][2][3]1/3 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemE[4][5][6]。体外研究L-Cystinehydrochloride(83µMor200µM,24hor72h)effectivelyreversesthegrowthinhibitionofbothSulfasalazine(HY-14655)andErastin(HY-15763)inHuh6cells[1].L-Cystinehydrochloride(0.8mM,4h)inducesNrf2proteinexpressionelevationinaKeap1-dependentmannerinHeLacells[6].CellViabilityAssay[1]CellLine:Huh6andHuh7cellsConcentration:83µMor200µMIncubationTime:72hor24hResult:ThedegreeofgrowthinhibitionbySulfasalazine(HY-14655)orErastin(HY-15763)wasgreaterinmediumcontainingaphysiologicallevelof83μML-Cystinehydrochloridethaninmediumcontaining200μML-CystinehydrochlorideinHuh6andHuh7cells.WesternBlotAnalysis[6]CellLine:HeLacellsConcentration:0.2mMIncubationTime:4hResult:WassufficientforproducingNrf2proteinelevationat0.2mMinHeLacells.WesternBlotAnalysis[6]CellLine:HeLacellsConcentration:0.8mMIncubationTime:4hResult:InducedNrf2inaKeap1-dependentmannerinHeLacells.体内研究L-Cystinehydrochloride(280mg/kg,p.o.,11days)administratedwithL-theanine(HY-15121)enhancesantigen-specificIgGproductionpartlythroughaugmentationofglutathione(GSH)levelsandThelper2(Th2)-mediatedresponsesinmice[2].L-Cystinehydrochloride(200mg/kg,p.o.,11days)administratedwithL-theanine(HY-15121)(80mg/kg,p.o.,11days)significantlydecreasestheserumIL-10/IFN-γratio6hoursafterDNP-KLHantigenicstimulation,butincreasestheserumIL-10/IFN-γratio24hoursafterDNP-KLHantigenicstimulationinmice[2].AnimalModel:Nine-week-oldfemaleBALB/cmice[2]Dosage:140,280and560mg/kg2/3 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemEAdministration: p.o.administrationfor11daysResult: SignificantlyincreasedIgGandIgMantibodyproductionadministratedwithL-theanine(HY-15121)afterDNP-dextranandDNP-KLHantigenicstimulationinmice.REFERENCESAbdullahM,etal.ExtracellularConcentrationofL-CystineDeterminestheSensitivitytoSystemxc-Inhibitors[J].BiomolTher(Seoul).2022Mar1;30(2):184-190.KuriharaS,etal.Enhancementofantigen-specificimmunoglobulinGproductioninmicebyco-administrationofL-cystineandL-theanine[J].JVetMedSci.2007Dec;69(12):1263-70.DominiqueBazin,etal.CystinuriaandcystinosisareusuallyrelatedtoL-cystine:isthisreallythecaseforcystinosis?Aphysicochemicalinvestigationatmicrometreandnanometrescale.ComptesRendus.Chimie,Volume25(2022)no.S1,pp.489-502.GrethWE,etal.CellularaccumulationofL-cystineinratkidneycortexinvivo[J].JClinInvest.1973Feb;52(2):454-62.OhtsuI,etal.UptakeofL-cystineviaanABCtransportercontributesdefenseofoxidativestressintheL-cystineexport-dependentmannerinEscherichiacoli.PLoSOne.2015Apr2;10(3):e0120619.DaiW,etal.FreshMediumorL-CystineasanEffectiveNrf2InducerforCytoprotectioninCellCulture[J].Cells.2023Jan12;12(2):291.M