白骨壤NAC家族成员鉴定和功能初探

[32]。RT-qRCR鉴定AmNAC基因在盐胁迫处理条件下的表达情况发现，盐胁迫下，白骨壤叶中有5个基因均在处理24h时呈现上调表达，并且基因AmNAC4被显著诱导，表达量约为对照组的3倍。这表明它们可能参与白骨壤应答盐胁迫过程，其中AmNAC4可能起关键作用。RT-qRCR结果与转录组数据不完全一致，可能是由于样品生长环境不同所致。已有多项研究证实，NAC转录因子通过调控盐胁迫相关基因的表达水平，引发一系列复杂的胁迫反应，从而提高植物应对高盐环境的能力。在植物盐胁迫调控中，这些NAC转录因子主要通过依赖ABA途径、不依赖ABA途径或两者共同参与的方式发挥作用REF_Ref163408844\r\h[33]。例如依赖ABA途径的水稻OsNAC3基因，通过调控ABA响应和耐盐相关基因的表达，提高植株对ABA的敏感性，从而增强植株的耐盐性REF_Ref163408898\r\h[34]。AmNAC基因共表达网络结果显示，5个盐胁迫响应AmNACs分别与多个白骨壤基因呈现共表达趋势。以上结果表明，作为转录因子，AmNACs可能通过调控下游基因的表达进而调控白骨壤盐胁迫响应过程。但AmNAC转录因子参与盐胁迫响应的途径及下游靶标基因的筛选仍有待进一步研究。本研究的成果为筛选受盐胁迫诱导的靶标基因、开展后续的遗传转化研究以及深入探索盐胁迫响应功能和分子调控机制奠定了理论基础。

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