进出口粮谷中玉米赤霉烯酮的测定 免疫亲合柱-液相色谱法

进出口粮谷中玉米赤霉烯酮的测定免疫亲和柱-液相色谱法b)流动相：乙腈+水+甲醇(46+46+8); —添加浓度在0.050mg/kg时，回收率为76.5%～90.3%;—滞加浓度在0.500mg/kg时，回收率为87.6%～98.2%。4.2.2小麦中玉米赤霹烯酮的添加浓度及回收率实验数据；—深加浓度在0.005mg/kg时，国收率为76.89%~90.7%;—深加浓度在0.050mg/kg时，回收率为78.3%～93.6%;—深加浓度在0.500mg/kg时，回收率为86,4%～93.2%。ThisstandardspeciflesthemethodofsamplumnandliquldchromatographyofzearalenoneinceThisstancardisapplicableforthedeterminationofzearalenonein2.1preparationoftestsieve,mixthoroughlyanddivideintotwooqualportions,Duringsam-plingandpreparationofsample,panyfactorsthatmaycausethechangeofThetestsampleshallbes3MethodofdetThezearalenoneinthetestsampleareextractedwithaimmunoaffinitycolumnforcleanup.Determinationismadeaquippedwithfluorescencedetector,usingexternalstaUnlessotherwisespecified,allreagentsshouldbeofanalyticalgradcorrespondingde-ionizedwater.3.2.2Acetonitrile;HPL3.2.3Acetonitrile-water(9+1):(90+10,V3.2.5Zearalenonestandard:Purity≥98%.6Zoaralenonestandardstoredsolution;Accuratelywoighfzoaralenonestandard,dissolveinacetonitrillutethestandardstocksolutionwithmobileph3.3.1Highperformanceliquidchromatographequippedwith3.3.8Immunoaffinitycolumn:ZearalenoneiWeigh40g(accurateto0.1g)ofthetes100mLacetonitrilewater(90+10,V/V)bybwasfilteredthroughfilterpaper,10.0mLoft 9X—theresiduecontentofzearalenoneinthetestsamA—thepeakareaofzearalemoneinthesaA₃—thepeakareaofzearalemoneintc—theconcentrationofzearalenoneinthestandardworkingsoluV—thefinalvolumeofthesamplesm—thecorrespondingmassofthetestsampleinthe4LimitofdetermiThelimitofdeterminat4.2.1Accordingtoexperimentaldata,thefortifyingcorrespondingreco—0.005mg/kg,therecover—0.050mg/kg,there—0.500