MCE 免疫检查点蛋白 Immune Checkpoint Proteins

www.MedChemEInhibitors • ScreeningLibraries • Proteins免疫检查点蛋白免疫检查点(ImmuneCheckpoints)分子是对免疫应答发挥抑制或刺激作用的配体-受体对，它们在维持免疫稳态和免疫耐受中起着重要的调节作用。大多数被报道的免疫检查点蛋白在适应性免疫系统（特别是T细胞）和先天免疫系统的细胞上表达。一些肿瘤细胞通过结合共抑制受体分子，限制正常的抗肿瘤免疫反应，从而帮助免疫逃逸。癌症的免疫检查点治疗策略包括了靶向这些调控途径，以恢复免疫细胞的抗肿瘤功能。目前，研究最广泛的抑制性免疫检查点包括CTLA-4和PD-1/PD-L1等[7][8]。A2aRVISTAAdenosineB7-H3PDL1/PDL2A2aRGalectin-9??CD80/CD86PD1TIM-3CD80/CD86CTLA-4OX40LCD28T-CellCD40OX40CD40LB7RP1ICOSCD70CD27HVEMBTLAAPCorLAG-3TumorCellTCRMHCl/llKIRGITRGITRL4-1BBCD226/TIGIT4-1BBLCD155/CD112OtherpotentialpathwaysIL10 CD47IDO TLRTGF-β ArginaseInhibitoryPathwaysStimulatoryPathwaysFigure1.常见的免疫检查点刺激途径和抑制途径[9]4MCEMasterofBioactiveMoleculesCat.No.ProductNameSpeciesSourceTagHY-P70691CTLA-4HumanMammalianCellsC-GSTHY-P70632PD-L1MouseMammalianCellsC-HisHY-P7395PD-1HumanMammalianCellsC-hFcHY-P70482TIM3HumanMammalianCellsC-HisHY-P70722LAG-3HumanMammalianCellsC-HisHY-P70624TIGITHumanMammalianCellsC-HisHY-P7327CD276/B7-H3HumanMammalianCellsC-HisHY-P74464-1BBL/TNFSF9MouseMammalianCellsN-HisHY-P7144CD40L/CD154/TRAPHumanE.coliTag-freeHY-P70652CD276/B7-H3HumanMammalianCellsC-HisHY-P7678BTLA/CD272HumanMammalianCellsC-HisHY-P7685BTN3A3HumanMammalianCellsC-HisHY-P70535Galectin-9HumanMammalianCellsC-HisHY-P7366HVEMHumanSf9insectCellsC-hFcHY-P70494Nectin-1HumanMammalianCellsC-HisHY-P70807PVR/CD155HumanMammalianCellsC-HisHY-P73370PD-L2RatMammalianCellsC-hFcHY-P77879VISTAHumanMammalianCellsC-hFcHY-P75610CD137/4-1BBCanineMammalianCellsC-HisHY-P7394OX40/TNFRSF4HumanMammalianCellsC-HisHY-P77457OX40Ligand/TNFSF4CynomolgusMammalianCellsN-mFcHY-P73499CD40HumanMammalianCellsC-HisHY-P73306Nectin-3HumanMammalianCellsC-HisHY-P71248PVRIGHumanMammalianCellsC-mFcHY-P76070SIRPalphaMouseMammalianCellsC-HisHY-P73121IDOHumanE.coliTag-freeHY-P76396ICOSHumanMammalianCellsC-His-hFcHY-P77575ICOSLGCynomolgusMammalianCellsC-HisHY-P72353CD28Human/CynomolgusMammalianCellsC-Fc-AviHY-P72033LIGHTHumanMammalianCellsN-hFc-MycHY-P73076GITRHumanMammalianCellsC-HisHY-P7318GITRL/AITRLHumanE.coliTag-freeHY-P72887CD200HumanMammalianCellsC-hFcHY-P76780CD200R1CynomolgusMammalianCellsC-His5www.MedChemEInhibitors • ScreeningLibraries • ProteinsMCE重组蛋白MCE重组蛋白产品种类丰富，涵盖细胞因子、病毒蛋白、免疫检查点蛋白、CAR-T蛋白、CD抗原、受体蛋白、生物素标记蛋白、GMP级蛋白、酶等多种分类，可用于人、病毒、小鼠、大鼠、猴、猪、狗、牛、羊、兔、猫、非洲爪蟾等多个物种不同研究领域的生物学研究，包括蛋白结构研究、免疫检测试剂、重组蛋白药物、诊断试剂开发、抗体药物靶点、CAR-T细胞治疗靶点、Fc受体、流感病毒蛋白和细胞因子等热门研究领域。MCE因高品质的产品和优质的售前售后服务，成为全球数百万计的科学家和技术人员值得信赖的实验伙伴。产品种类丰富： 细胞因子和生长因子、免疫检查点蛋白、CAR-T相关蛋白、CD抗原、受体蛋白、酶&调节子、补体系统蛋白、病毒类蛋白、生物素标记蛋白、荧光标记蛋白、GMP级蛋白,泛素相关蛋白等产品物种多样性：人、病毒、小鼠、大鼠、猴、猪、狗、牛、羊、兔、猫、非洲爪蟾、细菌、真菌等低内毒素水平： LAL法验证，内毒素含量低于绝大多数供应标准高纯度： SDS和HPLC检测验证高生物活性： 相关体内/体外生物学实验测定稳定的一致性： 多批次检测数据确认规格齐全： 满足客户的多种预分装规格高纯度ThepurityofhumanBMP-2isgreaterthan95%asanalyzedbySDSunderreducing(R)andnon-reducing(N)condition.kDa M R 120806040302010卓越的批间稳定性TheED50ofMCEhumanVEGF165fromthreedifferentLotsaresimilar.1.0Lot1nm)0.8Lot20.6Lot3(4900.4OD0.2Net0.01010101010VEGF165,Human(ng/mL)

高活性HumanGM-CSFstimulatescellproliferationofTF-1cellswithanED50oflessthan0.5ng/mL.2.0(490nm)1.51.0NetOD0.50.010101010101010GM-CSF,Human(ng/mL)竞品对比TheED50ofhumanVEGF165fromMCE’seachLotislowerthanofCompetitorP.1.0BrandPnm)0.8Lot20.6Lot3(4900.4OD0.2Net0.010-210-1101010VEGF165,Human(ng/mL)MCEMasterofBioactiveMoleculesPublicationsCitingUseofMCEProductsNature.2023Jun;618(7964):374-382.Nature.2023Jun;618(7966):862-870.Nature.2023Jun;618(7965):590-597.Nature.2023Jun;618(7963):180-187.Nature.2023May;617(7962):818-826Science.2023Jun9;380(6649):eabo2296.Science.2022Dec2;378(6623):eabo5503.Science.2022Nov18;378(6621):eabq7361.Science.2022Oct14;378(6616):eabq0132.Science.2022Jul8;377(6602):eabg9302Cell.2023Jun22;186(13):2823-2838.e20.Cell.2023May11;186(10):2144-2159.e22.Cell.2023Mar30;186(7):1352-1368.e18.Cell.2023Mar2;186(5):1026-1038.e20.Cell.2023Feb16;186(4):850-863.e16MCEGlobalPartnersMCEMasterofBioactiveMolecules客户验证诱导小鼠肺成纤维细胞纤维化TGF-β1(HY-P7117)刺激原代小鼠肺成纤维细胞，通过qRT-PCR和WB检测PTPN13和NOX4表达水平，确定其与细胞纤维化密切相关[41]。ControlTGF-β1PTPN13NOX4expression10**expression8**86mRNA6mRNA44Relative2Relative200

ControlTGF-β1ControlTGF-β1CollagenI139kDaPTPN13310kDaα-SMA42kDaNOX455kDaGAPDH37kDaGAPDH37kDa构建肾脏类器官从小鼠肾脏组织中提取细胞，利用微流体技术结合3D打印机制作类器官珠，辅以Noggin(HY-P7086)、R-spondin-1(HY-P7114)、FGF-4(HY-P7014)、FGF-basic(HY-P7066)、SB-431542(HY-10431)、Laduviglusib(HY-10182)培养，7天后，得到数千个大小、形状和成分具有高度重复性的肾脏类器官[42]。OrganoidGeneratingKidneyOrganoidBeadsKidneyOrganoidsMatrigel+CellsDay1Day7QDs/Drugs7days24hrsOrganoidCulturingCulturingHighThroughputScreeningOil4°CKidneyOrganoidBeads200μm200μmCultureDish37°CIFN-γ诱导蛋白16(IFI16)的积累通过Westernblotting检测IFN-γ(HY-P7025)处理人肺腺癌上皮(A549)细胞18小时后，蛋白16(IFI16)的积累，通过免疫染色检测IFN-γ诱导16小时后IFI16的细胞定位[43]。HumanIFN-γ(ng/mL)0505001000IFI16—100(kDa)—70β-actin—40

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