牛结核病防治技术规范样本

六、牛结核病防治技术规范牛结核病（BovineTuberculosis）是由牛型结核分枝杆菌（Mycobacteriumbovis）引起一种人兽共患慢性传染病，国内将其列为二类动物疫病。为了防止、控制和净化牛结核病，依照《中华人民共和国动物防疫法》及关于法律法规，特制定本规范。1合用范畴本规范规定了牛结核病诊断、疫情报告、疫情解决、防治办法、控制和净化原则。本规范合用于中华人民共和国境内从事饲养、生产、经营牛及其产品，以及从事有关动物防疫活动单位和个人。2诊断2.1流行特点本病奶牛最易感，另一方面为水牛、黄牛、牦牛。人也可被感染。结核病病牛是本病重要传染源。牛型结核分枝杆菌随鼻汁、痰液、粪便和乳汁等排出体外，健康牛可通过被污染空气、饲料、饮水等经呼吸道、消化道等途径感染。2.2临床特性：潜伏期普通为3～6周，有可长达数月或数年。临床普通呈慢性通过，以肺结核、乳房结核和肠结核最为常用。肺结核：以长期顽固性干咳为特性，且以清晨最为明显。患畜容易疲劳，逐渐消瘦，病情严重者可见呼吸困难。乳房结核：普通先是乳房淋巴结肿大，继而后方乳腺区发生局限性或弥漫性硬结，硬结无热无痛，表面凹凸不平。泌乳量下降，乳汁变稀，严重时乳腺萎缩，泌乳停止。肠结核：消瘦,持续下痢与便秘交替浮现，粪便常带血或脓汁。2.3病理变化：在肺脏、乳房和胃肠粘膜等处形成特异性白色或黄白色结节。结节大小不一，切面干酪样坏死或钙化，有时坏死组织溶解和软化，排出后形成空洞。胸膜和肺膜可发生密集结核结节，形如珍珠状。2.4实验室诊断2.4.1病原学诊断采集病牛病灶、痰、尿、粪便、乳及其他分泌物样品，作抹片或集菌解决（见附件）后抹片，用抗酸染色法染色镜检，并进行病原分离培养和动物接种等实验。2.4.2免疫学实验牛型结核分枝杆菌PPD（提纯蛋白衍生物）皮内变态反映实验（即牛提纯结核菌素皮内变态反映实验）（见GB/T18646）。2.5成果鉴定本病根据流行病学特点、临床特性、病理变化可做出初步诊断。确诊需进一步做病原学诊断或免疫学诊断。2.5.1分离出结核分枝杆菌（涉及牛结核分枝杆菌、结核分枝杆菌）判为结核病牛。2.5.2牛型结核分枝杆菌PPD皮内变态反映实验阳性牛，判为结核病牛。3疫情报告3.1任何单位和个人发现疑似病牛，应当及时向本地动物防疫监督机构报告。3.2动物防疫监督机构接到疫情报告并确认后，按《动物疫情报告管理办法》及关于规定及时上报。4疫情解决4.1发现疑似疫情，畜主应限制动物移动；对疑似患病动物应及时隔离。4.2动物防疫监督机构要及时派员到现场进行调查核算，开展实验室诊断。确诊后，本地人民政府组织关于部门按下列规定解决：4.2.1扑杀对患病动物所有扑杀。4.2.2隔离对受威胁畜群（病畜同群畜）实行隔离，可采用圈养和固定草场放牧两种方式隔离。隔离饲养用草场，不要接近交通要道，居民点或人畜密集地区。场地周边最佳有自然屏障或人工栅栏。对隔离畜群结核病净化，按本规范5.5规定进行。4.2.3无害化解决病死和扑杀病畜，要按照GB16548-1996《畜禽病害肉尸及其产品无害化解决规程》进行无害化解决。4.2.4流行病学调查及检测开展流行病学调查和疫源追踪；对同群动物进行检测。4.2.5消毒对病畜和阳性畜污染场合、用品、物品进行严格消毒。饲养场金属设施、设备可采用火焰、熏蒸等方式消毒；养畜场圈舍、场地、车辆等，可选用2%烧碱等有效消毒药消毒；饲养场饲料、垫料可采用深埋发酵解决或焚烧解决；粪便采用堆积密封发酵方式，以及其他相应有效消毒方式。4.2.6发生重大牛结核病疫情时，本地县级以上人民政府应按照《重大动物疫情应急条例》关于规定，采用相应疫情扑灭办法。5防止与控制采用以“监测、检疫、扑杀和消毒”相结合综合性防治办法。5.1监测监测对象：牛监测比例为：种牛、奶牛100%，规模场肉牛10%，其他牛5%，疑似病牛100%。如在牛结核病净化群中（涉及犊牛群）检出阳性牛时，应及时扑杀阳性牛，其他牛按假定健康群解决。成年牛净化群每年春秋两季用牛型结核分枝杆菌PPD皮内变态反映实验各进行一次监测。初生犊牛，应于20日龄时进行第一次监测。并按规定使用和填写监测成果报告，及时上报。5.2检疫异地调运动物，必要来自于非疫区，凭本地动物防疫监督机构出具检疫合格证明调运。动物防疫监督机构应对调运种用、乳用、役用动物进行实验室检测。检测合格后，方可出具检疫合格证明。调入后应隔离饲养30天，经本地动物防疫监督机构检疫合格后，方可解除隔离。5.3人员防护饲养人员每年要定期进行健康检查。发现患有结核病应调离岗位，及时治疗。5.4防疫监督结核病监测合格应为奶牛场、种畜场《动物防疫合格证》发放或审验必备条件。动物防疫监督机构要对辖区内奶牛场、种畜场检疫净化状况监督检查。鲜奶收购点（站）必要凭奶牛健康证明收购鲜奶。5.5净化办法被确诊为结核病牛牛群（场）为牛结核病污染群（场），应所有实行牛结核病净化。5.5.1牛结核病净化群（场）建立污染牛群解决：应用牛型结核分枝杆菌PPD皮内变态反映实验对该牛群进行重复监测，每次间隔3个月，发现阳性牛及时扑杀，并按照本规范4规定解决。犊牛应于20日龄时进行第一次监测，100～120日龄时，进行第二次监测。凡持续两次以上监测成果均为阴性者，可以为是牛结核病净化群。凡牛型结核分枝杆菌PPD皮内变态反映实验疑似反映者，于42天后进行复检，复检成果为阳性，则按阳性牛解决；若仍呈疑似反映则间隔42天再复检一次，成果仍为可疑反映者，视同阳性牛解决。5.5.2隔离疑似结核病牛或牛型结核分枝杆菌PPD皮内变态反映实验可疑畜须隔离复检。5.5.3消毒暂时消毒：奶牛群中检出并剔出结核病牛后，牛舍、用品及运动场合等按照4.2.5规定进行紧急解决。经常性消毒：饲养场及牛舍出入口处，应设立消毒池，内置有效消毒剂，如3～5%来苏尔溶液或20％石灰乳等。消毒药要定期更换，以保证一定药效。牛舍内一切用品应定期消毒;产房每周进行一次大消毒，分娩室在临产牛生产前及分娩后各进行一次消毒。

附件样品集菌方法痰液或乳汁等样品，由于含菌量较少，如直接涂片镜检往往是阴性成果。此外，在培养或作动物实验时，常因污染杂菌生长较快，使病原结核分枝杆菌被抑制。下列几种消化浓缩办法可使检查标本中蛋白质溶解、杀灭污染杂菌，而结核分枝杆菌因有蜡质外膜而不死亡，并得到浓缩。1硫酸消化法用4－6％硫酸溶液将痰、尿、粪或病灶组织等按1:5之比例加入混合，然后置37℃作用1－2小时，经3000－4000rpm离心30分钟，弃上清，取沉淀物涂片镜检、培养和接种动物。也可用硫酸消化浓缩后，在沉淀物中加入3％氢氧化钠中和，然后抹片镜检、培养和接种动物。2氢氧化钠消化法取氢氧化钠35－40g，钾明矾2g，溴麝香草酚兰20mg（预先用60％酒精配制成0.4％浓度，应用时按比例加入），蒸馏水1000mL混合，即为氢氧化钠消化液。将被检痰、尿、粪便或病灶组织按1:5比例加入氢氧化钠消化液中，混匀后，37℃作用2－3小时，然后无菌滴加5－10％盐酸溶液进行中和，使标本pH调到6.8左右（此时显淡黄绿色），以3000－4000rpm离心15－20分钟，弃上清，取沉淀物涂片镜检、培养和接种动物。在病料中加入等量4％氢氧化钠溶液，充分振摇5－10分钟，然后用3000rpm离心15－20分钟，弃上清，加1滴酚红批示剂于沉淀物中，用2N盐酸中和至淡红色，然后取沉淀物涂片镜检、培养和接种动物。在痰液或小脓块中加入等量1％氢氧化钠溶液，充分振摇15分钟，然后用3000rpm离心30分钟，取沉淀物涂片镜检、培养和接种动物。对痰液消化浓缩也可采用如下较温和解决办法：取1N（或4％）氢氧化钠水溶液50mL，0.1mol/L柠檬酸钠50mL，N－乙酰－L－半胱氨酸0.5g，混合。取痰一份，加上述溶液2份，作用24－48小时，以3000rpm离心15分钟，取沉淀物涂片镜检、培养和接种动物。3安替福民（Antiformin）沉淀浓缩法溶液A：碳酸钠12g、漂白粉8g、蒸馏水80mL。溶液B：氢氧化钠15g、蒸馏水85mL。应用时A、B两液等量混合，再用蒸馏水稀释成15－20％后使用，该溶液须存储于棕色瓶内。将被检样品置于试管中，加入3－4倍量15－20％安替福民溶液，充分摇匀后37℃作用1小时，加1－2倍量灭菌蒸馏水，摇匀，3000－4000rpm离心20－30分钟，弃上清沉淀物加蒸馏水恢复原量后再离心一次，取沉淀物涂片镜检、培养和接种动物。PrescribedandalternativediagnostictestsforOIElisteddiseasesTerrestrialCode

ChapterNo.TerrestrialManual

ChapterNo.Disease

namePrescribed

testsAlternative

tests.7.BovinetuberculosisTuberculintestGammainterferontestCountryorzonefreefrombovinetuberculosisToqualifyasfreefrombovinetuberculosis，acountryorzoneshouldsatisfythefollowingrequirements:M.bovisinfectionindomestic(permanentlycaptiveandownedfree-range)bovinesincludingcattle，waterbuffaloandwoodbisonisanotifiablediseaseinthecountry;anon-goingawarenessprogrammeshouldbeinplacetoencouragereportingofallcasessuggestiveofbovinetuberculosis;regularandperiodictestingofallcattle，waterbuffalo，andwoodbisonherdsdemonstratedthatM.

bovisinfectionwasnotpresentinatleast99.8%oftheherdsand99.9%ofthecattle，waterbuffaloandwoodbisoninthecountryorzonefor3

consecutiveyears;asurveillanceprogrammeshouldbeinplacetodetectbovinetuberculosisinthecountryorzonethroughante-mortemandpost-morteminspectionasdescribedinChapter

6.2.;ifthesurveillanceprogrammedescribedinpoints

3and4abovehasnotdetectedinfectionwithM.

bovisfor5

consecutiveyears，surveillancemaybemaintainedthroughante-mortemandpost-morteminspectionasdescribedinChapter6.2.;cattle，waterbuffaloandwoodbisonintroducedintoacountryorzonefreefrombovinetuberculosisshouldbeaccompaniedbyacertificatefromanOfficialVeterinarianattestingthattheycomefromacountry，zone，compartmentorherdfreefrombovinetuberculosisorcomplywiththerelevantprovisionsinArticle

11.7.5.orinArticle

11.7.6.Chapter11.7.bovinetuberculosisArticle11.7.1.GeneralprovisionsTherecommendationsinthischapterareintendedtomanagethehumanandanimalhealthrisksassociatedwithMycobacteriumbovis(M.

bovis)infectionindomestic(permanentlycaptiveandownedfree-range)bovinesincludingcattle(Bostaurus，B.

indicusandB.

grunniens)，waterbuffaloes(Bubalusbubalis)andwoodbisons(BisonbisonandB.bonasus).StandardsfordiagnostictestsaredescribedintheTerrestrialManual.Article11.7.2.CountryorzonefreefrombovinetuberculosisToqualifyasfreefrombovinetuberculosis，acountryorzoneshouldsatisfythefollowingrequirements:M.bovisinfectionindomestic(permanentlycaptiveandownedfree-range)bovinesincludingcattle，waterbuffaloandwoodbisonisanotifiablediseaseinthecountry;anon-goingawarenessprogrammeshouldbeinplacetoencouragereportingofallcasessuggestiveofbovinetuberculosis;regularandperiodictestingofallcattle，waterbuffalo，andwoodbisonherdsdemonstratedthatM.

bovisinfectionwasnotpresentinatleast99.8%oftheherdsand99.9%ofthecattle，waterbuffaloandwoodbisoninthecountryorzonefor3

consecutiveyears;asurveillanceprogrammeshouldbeinplacetodetectbovinetuberculosisinthecountryorzonethroughante-mortemandpost-morteminspectionasdescribedinChapter

6.2.;ifthesurveillanceprogrammedescribedinpoints

3and4abovehasnotdetectedinfectionwithM.

bovisfor5

consecutiveyears，surveillancemaybemaintainedthroughante-mortemandpost-morteminspectionasdescribedinChapter6.2.;cattle，waterbuffaloandwoodbisonintroducedintoacountryorzonefreefrombovinetuberculosisshouldbeaccompaniedbyacertificatefromanOfficialVeterinarianattestingthattheycomefromacountry，zone，compartmentorherdfreefrombovinetuberculosisorcomplywiththerelevantprovisionsinArticle

11.7.5.orinArticle

11.7.6.Article11.7.3.CompartmentfreefrombovinetuberculosisToqualifyasacompartmentfreefrombovinetuberculosis，cattle，waterbuffaloorwoodbisoninacompartmentshouldbecertifiedbytheVeterinaryAuthorityassatisfyingthefollowingrequirements:cattle，waterbuffaloandwoodbison:showednosignofbovinetuberculosisorlesionsatante-mortemorpost-morteminspectionforatleast3

consecutiveyears;wereover6

weeksofageatthetimeofthefirsttestandhaveshownanegativeresulttoatleasttwotuberculintestscarriedoutatanintervalofaminimumof6

months，thefirsttestbeingperformedatleast6

monthsfollowingtheslaughterofthelastaffectedanimal;metoneofthefollowingconditions:showedanegativeresulttoabiannualtuberculintesttoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisismorethan1%ofallherdsinthecountryorzoneduringthelast2

years；orshowedanegativeresulttoanannualtuberculintesttoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisismorethan0.2%butnotmorethan1%ofallherdsinthecountryorzoneduringthelast2

years；orshowedanegativeresulttoatuberculintestevery3

yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan0.2%ofallherdsinthecountryorzoneduringthelast4

years；orshowedanegativeresulttoatuberculintestevery4

yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan0.1%ofallherdsinthecountryorzoneduringthelast6years;cattle，waterbuffaloandwoodbisonintroducedintothecompartmentcomefromaherdfreefrombovinetuberculosis.Thisconditionmaybewaivedforanimalswhichhavebeenisolatedforatleast90

daysandwhich，priortoentryintothecompartment，weresubjectedtoatleasttwotuberculintestscarriedoutata6-monthintervalwithnegativeresultswiththesecondtuberculintestperformedduringthe30dayspriortoentryintothecompartment;cattle，waterbuffaloandwoodbisoninacompartmentfreefrombovinetuberculosisareprotectedfromcontactwithwildlifereservoirsofbovinetuberculosisandaremanagedunderacommonbiosecurityplanprotectingthemfromcontaminationwithM.

bovis，andthecompartmenthasbeenapprovedbytheVeterinaryAuthorityinaccordancewithChapters

4.3.and4.4.Article11.7.4.HerdfreefrombovinetuberculosisToqualifyasfreefrombovinetuberculosis，aherdofcattle，waterbuffalo，orwoodbisonsshouldsatisfythefollowingrequirements:theherdisinacountry，zoneorcompartmentfreefrombovinetuberculosisandiscertifiedfreebytheVeterinaryAuthority；orcattle，waterbuffaloandwoodbisonintheherd:showednosignsofbovinetuberculosisorlesionsatante-mortemorpost-morteminspectionforatleast3

consecutiveyears;wereover6

weeksofageatthetimeofthefirsttestandhaveshownanegativeresulttoatleasttwotuberculintestscarriedoutatanintervalofaminimumof6months，thefirsttestbeingperformedatleast6monthsfollowingtheslaughterofthelastaffectedanimal;metoneofthefollowingconditions:showedanegativeresulttoanannualtuberculintesttoensurethecontinuingabsenceofbovinetuberculosis；orshowedanegativeresulttoatuberculintestevery2yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan1%ofallherdsinthecountryorzoneduringthelast2years；orshowedanegativeresulttoatuberculintestevery3yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan0.2%ofallherdsinthecountryorzoneduringthelast4years；orshowedanegativeresulttoatuberculintestevery4yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan0.1%ofallherdsinthecountryorzoneduringthelast6years;cattle，waterbuffaloandwoodbisonintroducedintotheherdcomefromaherdfreefrombovinetuberculosis.Thisconditionmaybewaivedforanimalswhichhavebeenisolatedforatleast90daysandwhich，priortoentryintotheherd，weresubjectedtoatleasttwotuberculintestscarriedoutata6-monthintervalwithnegativeresults.Article11.7.5.Recommendationsfortheimportationofcattle，waterbuffaloandwoodbisonforbreedingorrearingVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthattheanimals:showednosignsofbovinetuberculosisonthedayofshipment;originatefromaherdfreefrombovinetuberculosisthatisinacountry，zoneorcompartmentfreefrombovinetuberculosis；orweresubjectedtothetuberculintestforbovinetuberculosiswithnegativeresultsduringthe30dayspriortoshipmentandcomefromaherdfreefrombovinetuberculosis；orhavebeenisolatedforatleast90dayspriortoentryintotheherdincludingprotectionfromcontactwithwildlifereservoirsofbovinetuberculosisandweresubjectedtoatleasttwotuberculintestscarriedoutatasix-monthintervalwithnegativeresultswiththesecondtuberculintestperformedduringthe30dayspriortoentryintotheherd.Article11.7.6.Recommendationsfortheimportationofcattle，waterbuffaloandwoodbisonforslaughterVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthattheanimals:showednosignsofbovinetuberculosisonthedayofshipment;originatedfromaherdfreefrombovinetuberculosisorweresubjectedtoatuberculintestforbovinetuberculosiswithnegativeresultsduringthe30dayspriortoshipment;werenotbeingeliminatedaspartofaneradicationprogrammeagainstbovinetuberculosis.Article11.7.7.Recommendationsfortheimportationofsemenofcattle，waterbuffaloandwoodbisonVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthat:thedonoranimalsshowednosignsofbovinetuberculosisonthedayofcollectionofthesemenandeither:werekeptinanartificialinseminationcentrefreefrombovinetuberculosisinacountry，zoneorcompartmentfreefrombovinetuberculosisandwhichonlyacceptsanimalsfromfreeherdsinafreecountry，zoneorcompartment；orshowednegativeresultstotuberculintestscarriedoutannuallyandwerekeptinaherdfreefrombovinetuberculosis;thesemenwascollected，processedandstoredinconformitywiththeprovisionsofChapter

4.5.andChapter

4.6.Article11.7.8.Recommendationsfortheimportationofembryos/ovaofcattle，waterbuffaloandwoodbisonVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthat:thedonorfemalesandallothersusceptibleanimalsintheherdoforiginshowednosignsofbovinetuberculosisduringthe24

hourspriortoembryocollection；andeitheroriginatedfromaherdfreefrombovinetuberculosisinacountry，zoneorcompartmentfreefrombovinetuberculosis；orwerekeptinaherdfreefrombovinetuberculosis，andweresubjectedtoatuberculintestforbovinetuberculosiswithnegativeresultsduringanisolationperiodof30daysintheestablishmentoforiginpriortocollection;theembryos/ovawerecollected，processedandstoredinconformitywiththeprovisionsofChapters

4.7.，4.8.and4.9.，asrelevant.Article11.7.9.Recommendationsfortheimportationoffreshmeatandmeatproductsofcattle，waterbuffalo，andwoodbisonVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthattheentireconsignmentofmeatcomesfromanimalswhichhavebeensubjectedtoante-mortemandpost-morteminspectionsasdescribedinChapter

6.2.Article11.7.10.Recommendationsfortheimportationofmilkandmilkproductsofcattle，waterbuffaloandwoodbisonVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthattheconsignment:hasbeenderivedfromanimalsinaherdfreefrombovinetuberculosis；orwassubjectedtopasteurization；orwassubjectedtoacombinationofcontrolmeasureswithequivalentperformanceasdescribedintheCodexAlimentariusCodeofHygienicPracticeforMilkandMilkProducts.第11.6。

牛结核病

第11.6.1。

普通规定在本章建议是为了管理人类和动物健康有关风险，牛分枝杆菌分枝杆菌感染（永久圈养和国有散养）在国内牛，涉及牛（牛，B.蜓和B.牦牛），水牛（水牛）和木野牛（野牛野牛B.bonasus）。诊断测试原则描述在陆地手册。

第11.6.2。

牛结核病国家或区域牛结核病，一种国家或区域有资格作为应符合下列规定：牛分枝杆菌

感染，在国内（永久圈养和散养国有）牛涉及黄牛，水牛和木野牛是在该国须呈报疾病

;持续宣传方案要到位，以勉励所有报告暗示牛结核病案件

;所有牛​​，水牛和木野牛经常和定期检测牛群表白研究

杆菌

感染是当前至少有99.8％牛群和99.9％牛，水牛和木野牛国家或区域持续三年;一种监视程序要到位。检测牛结核病，在通过宰前和宰后检查国家或区域在第一章描述

6.2

;如果监察筹划分3和第4段中所述证明，M。杆菌

感染是当前至少有99.8％牛群和99.9％牛，水牛和木野牛国家或区域持续五年，监视也许会维持通过宰前和宰后检查中所述第

6.2章

;牛，水水牛和木野牛引入到一种国家或区牛结核病，应随着着从一种证书证明，她们从一种国家官方兽医，区，车厢或无牛结核病牛群，或遵守关于规定在第

11.6.5

或11.6.6条

。

第11.6.3。

无隔间牛结核病有资格作为一种车厢，牛结核病，牛，水牛或在木野牛舱应当由兽医管理局认证，符合下列规定：黄牛，水牛和木野牛：至少持续三年没有在事前或宰后检查表白牛结核病或病变迹象;在第一次测试时间超过6周龄，并已显示出负面成果至少有两个结核菌素至少半年时间间隔进行测试，第一次测试正在执行至少6个月如下虐杀最后受影响

动物

;符合下列条件之一：每年两次结核菌素实验呈阴性成果，以保证牛结核病持续状况下，如果牛群证明感染肺结核年度比例超过1％牛群，在过去两年，在国家或区域

;呈阴性成果以每年结核菌素实验，以保证持续状况下，如果牛结核病年度比例牛群证明感染肺结核是0.2％以上，但不超过1％牛群在国家或区域在过去两年;显示了一种结核菌素实验阴性成果每三年，以保证持续状况下，牛结核病，如果牛群证明感染肺结核年度比例不超过0.2％牛群在过去四年在全国或区域

;或显示了一种结核菌素实验阴性成果每四年，以保证持续状况下，牛结核病，如果不超过0.1％牛群在国家或区