医学医药生物技术diversity of cultivated bcteria and actinobacteria three different types specific habitat

vvitf)d•2typicalkindsofresourcefromtTypesofSpecificBiologocalscreen玉••Taxo国院医药生物技术研究2015-08-1MAINEndophyticActinobacteriafromMAINEndophyticActinobacteriafromMidicinal12BacteriafromtheMicroecosystemRelatedto321EndophyticActinobacteriafrom1EndophyticActinobacteriafromMidicinalBalancedantagonismV.GangadeviandJ.Muthumary.TaxolproductionbyPestalotiopsisterminaliae,anendophyticfungusofTerminaliaarjuna(arjuntree).BiotechnologyandAppliedBiochemistry.52(1),3494043047349404304734Generaof5Micromonaspora19Asanoasp.I10A-Pseudonocardia3Rhodococcus3Mycobacterium4Microbispora2Streptosporangiumsp.I10A-34Nocardiopsis12Labedella3Agrococcus3Curtobacterium4Frigoribacteriumsp.I10A-Herbiconiux3Microbacterium5Cellulomonassp.I10A-Brachybacterium6Promicromonospora3Micrococcus4Kocuriasp.I10A-Friedmanniella4Streptomyces7Methylobacterium5G-BacteriaMicromonaspora19Asanoasp.I10A-Pseudonocardia3Rhodococcus3Mycobacterium4Microbispora2Streptosporangiumsp.I10A-34Nocardiopsis12Labedella3Agrococcus3Curtobacterium4Frigoribacteriumsp.I10A-Herbiconiux3Microbacterium5Cellulomonassp.I10A-Brachybacterium6Promicromonospora3Micrococcus4Kocuriasp.I10A-Friedmanniella4Streptomyces7Methylobacterium5G-Bacteria(9.1%Rhodopseudomonas2Stenotrophomonassp.I10A-Pseudomonas2Adhaeribacter.spI10B-Paenibacillus4LowG+CcontentG+Bacillus5Staphylococcus667BIOLOGGENIIIAPI50CH7BIOLOGGENIIIAPI50CHAPI30291422DendrogramsDendrogramsofthetestedstrainsbasedonthephysiologicalcharacteristics(A),8ActaMicrobiologicaSinica2013(1):15-Streptomycesspp.(23Streptomycessp.I13A-01606Streptomycesspp.(2strains)Kribbellaspp.(4Micromonosporaspp.(2Amycolatopsisspp.(7Nocardiaspp.(799Rhodococcusspp.(3Rhodococcusspp.(2 Arthrobacterspp.(2Kineococcusspp.(2Terrabacterspp.(7Cellulomonasspp.(7Cellulomonassp.1Oerskoviaspp.(7Frigoribacteriumsp.1Streptomycesspp.(23Streptomycessp.I13A-01606Streptomycesspp.(2strains)Kribbellaspp.(4Micromonosporaspp.(2Amycolatopsisspp.(7Nocardiaspp.(799Rhodococcusspp.(3Rhodococcusspp.(2 Arthrobacterspp.(2Kineococcusspp.(2Terrabacterspp.(7Cellulomonasspp.(7Cellulomonassp.1Oerskoviaspp.(7Frigoribacteriumsp.1Frondihabitansspp.(596Curtobacteriumspp.(7Curtobacteriumspp.(2Leifsoniaspp.(20Tardiphagasp.1Burkholderiasp.1Dyellaspp.(3Paenibacillusspp.(3Bacillusspp.(2Lysinibacillusspp.(4Phylogeneticdendrogrambasedon16SrRNAgenesequencesanalysisofpartialisolatesandrelatedstrains.Bootstrapvaluesbasedon1000resampleddatasetsareatbranchnodes.Bar,0.02substitutionsper93562641thepotentialnovelActinobacteriataxafromthepotentialnovelActinobacteriataxafromHuperziaserrataEndophyticBacteriafromHuperziaShannon-wienerIndex(H),SimpsonIndex(D)andPielouIndexoftheEndophyticBacteriaEndophyticBacteriafromHuperziaShannon-wienerIndex(H),SimpsonIndex(D)andPielouIndexoftheEndophyticBacteriafromdifferentpartsofHuperziaJaccardindex:3562641Microbacteriumspp.Index(H)SimpsonIndexPielouIndex282941(16generainPhysiologicalactivitiesscreeningresultsoftheendophyticbacteriafrommedicinalPhysiologicalactivitiesscreeningresultsoftheendophyticbacteriafrommedicinalfaecalissalmonicolorAnti-ShapotouNDER,thefirstNationalDesertShapotouNDER,thefirstNationalDesertEcologicalReservealtitudeof1300-1700m,186.2mmofprecipitation,anannualaveragespeedof2.8ThediversityofculturableactinobacteriainShapotou 沙面结皮形成与微环境变化[J].应用生态学报1993,393-CC,Cyanobacteria-dominatedsoilcrusts;MC,Moss-dominatedsoilcrusts;BS,BareInformationregardingInformationregardingthe50samplescollectedinShapotouKibdelosporangiumsp.I12A-02945Actinomycetosporaspp.(2strains)Blastococcusspp.(3 Blastococcusspp.(3210Actinoplanessp.I12A-02925Micromonosporaspp.(2strains)18Micromonosporaspp.(2strains)29 Okibacteriumspp.(2strains)Cellulomonasspp.(6strains)Kribbellaspp.(14strains)Nonomuraeaspp.(2strains)Kibdelosporangiumsp.I12A-02945Actinomycetosporaspp.(2strains)Blastococcusspp.(3 Blastococcusspp.(3210Actinoplanessp.I12A-02925Micromonosporaspp.(2strains)18Micromonosporaspp.(2strains)29 Okibacteriumspp.(2strains)Cellulomonasspp.(6strains)Kribbellaspp.(14strains)Nonomuraeaspp.(2strains)Glycomycesspp.(3EsteraseasEsteraseasanenzymaticsignatureofmonumens.ofAppliedMicrobiology,2010,108:1723–1732.API50CHAPIAntibiotic34Blastococcus11GeodermatophilusBioactivityPhysiologicalactivitiesscreeningresultsoftheactinobacteriafromShapotouBioactivityPhysiologicalactivitiesscreeningresultsoftheactinobacteriafromShapotoufaecalissalmonicolorAnti-aI12A-I12A-I12A-I12A-bI12A-(ExceptGeodermatophilussp.I12A-IBlastococcusModestobacter02622andI12A-02624)IBlastococcusModestobacterDendrogrambasedonthephysiologicalcharacteristicsprofilesofthetestedstrains.Note:Differentcolorsdenotethestrainsisolatedfromdifferenttypesofsamples.Blue,Cyanobacteria-dominatedsoilcrusts;Green,Moss-dominatedsoilcrusts;Red,Lichen-dominatedsoilcrusts;Black,BareI12A-I12A-I11A-aI12A-I12A-I12A-I12A-bI12A-(ExceptGeodermatophilussp.I12A-IBlastococcusModestobacter02622andI12A-02624)IBlastococcusModestobacterDendrogrambasedonthephysiologicalcharacteristicsprofilesofthetestedstrains.Note:Differentcolorsdenotethestrainsisolatedfromdifferenttypesofsamples.Blue,Cyanobacteria-dominatedsoilcrusts;Green,Moss-dominatedsoilcrusts;Red,Lichen-dominatedsoilcrusts;Black,BareI12A-I12A-I11A-I12A-I12A-I12A-I12A-I12A-I12A-I12A-I12A-I12A-I12A-I11A-I12A-40Families,62Genera40Families,62Genera(5GeneraofIsolationandIdentificationofNovelMicrocystinDegradingIsolationandIdentificationofNovelMicrocystinDegradingBacteria.AEM2009,6924-MicrocystindegradingBifidobacteriumspp.Microbacteriumspp.Arthrobacterspp.BrevibacteriumRhodococcusBacillusLactobacillusRhizobiumSphingomonasSphingopyxisα-Ralstoniaspp.Burkholderiaspp.Methylobacillusspp.Paucibacterβ-PseudomonasStenotrophomonasMorganellaγ-Streptomyces?Streptomyces?SUMMARY&ASUMMARY&AbatchofinterestingbacteriawithpotentialfounctionhaveSomenovelmicrobialtaxahavebeenidentifiedandThemechanismsoftheroleswhichthemicrobesplayedinthemicroecosystemswillberevealedusingthesescreenedNoveltaxaThemostrelated16SrRNAgeneCPCCNocardioideskoreensisMSL-CPCCNocardiopsisNoveltaxaThemostrelated16SrRNAgeneCPCCNocardioideskoreensisMSL-CPCCNocardiopsisarabiaCPCCAngustibacteraerolatus7402J-CPCCKineosporiarhamnosaJCMCPCCJatrophihabitansendophyticusCPCCCadifontibacterliindicusPCCPCCActinophytocolaoryzaeCPCCActinopolymorphaalbaYIMCPCCModestobactermultiseptatusAA-CPCCModestobactermultiseptatusAA- CPCCNocardioidesbasaltisJ112TNocardioidessalariusCL-Z59TNocardioidesmarinisabuliSBS-12TNocardioidesdokdonensisFR1436TNocardioidesginsengagriBX5-10TNocardioidesplantarumNCIMB1234TNocardioidescaricicolaYC6903TNocardioideskribbensisKSL-2TNocardioidesaquaticusEL-17KTNocardioidesbigeumensisMSL-19TCPCCNocardioidesbasaltisJ112TNocardioidessalariusCL-Z59TNocardioidesmarinisabuliSBS-12TNocardioidesdokdonensisFR1436TNocardioidesginsengagriBX5-10TNocardioidesplantarumNCIMB1234TNocardioidescaricicolaYC6903TNocardioideskribbensisKSL-2TNocardioidesaquaticusEL-17KTNocardioidesbigeumensisMSL-19TNocardioidesislandensisMSL26TNocardioidesagariphilusMSL28TNocardioidesginsengisegetisKCTC19469TNocardioideskoreensisKCTC19272TNocardioidesperillaeCPCC203382TNocardioidesalkalitoleransKCTC19037TNocardioideslentusKSL-17TActinosingapurapolymorphaMDN22TIntJSystEvolMicrobiol.2013,63(3):1068-CPCCStreptomonosporahalophilaYIM91355TNocardiopsisarabiaS186T 100 StreptomonosporaalbaYIM90003T StreptomonosporasalinaYIM90002TNocardiopsistrehalosiVKMAc-942THaloactinosporaalbaYIM90648TMarinactinosporaCPCCStreptomonosporahalophilaYIM91355TNocardiopsisarabiaS186T 100 StreptomonosporaalbaYIM90003T StreptomonosporasalinaYIM90002TNocardiopsistrehalosiVKMAc-942THaloactinosporaalbaYIM90648TMarinactinosporathermotoleransSCSIO00652TThermobifidahalotoleransYIM90462TThermobifidaalbaJCM3077TThermobifidafuscaATCC27730TThermobifidacellulosilyticaTB100TCPCC203428TThermomonosporaceaeIntJSystEvolMicrobiol.2013,63(3):900-I10A-abcI10A-abcHPLC-CPCCActinophytocolaoryzaeNBRC105245CPCCActinophytocolaxinjiangensisNBRC106673AmycolatopsisorientalisIMSNU20058TAJ400711)Amycolatopsisminnesotaensis32U-2T(DQ076482)Amycolatopsissaalfeldensis2406-001T(DQ792500) CPCCActinophytocolaoryzaeNBRC105245CPCCActinophytocolaxinjiangensisNBRC106673AmycolatopsisorientalisIMSNU20058TAJ400711)Amycolatopsisminnesotaensis32U-2T(DQ076482)Amycolatopsissaalfeldensis2406-001T(DQ792500) KibdelosporangiumphilippinenseDSM44226PseudonocardiacarboxydivoransY8StreptoalloteichushindustanusATCC31158StreptomycesambofaciensDSM40697CPCCMarmoricolaaurantiacusBC361TMarmoricolascoriaeSco-D01TMarmoricolakorecusSco-A36TMarmoricolaaequoreusSST-45TMarmoricolabigeumensisMSL-05TMumiaflavaMUSC201TNocardioidesplantarumNCIMB12834TNocardioidesalbusKCTC9186T NocardioidessimplexKCTC9106TAeromicrobiumerythreumNRRLB-3381TAeromicrobiumfastidiosumJ41TAeromicrobiummarinumDSM15272TCPCCMarmoricolaaurantiacusBC361TMarmoricolascoriaeSco-D01TMarmorico