微珠图案上有序排列的孔的多孔膜

MicrobeadPatterningonPorousFilmwithOrderedArraysofPores

Reporter:LeiZhangLeiLiuJianhengLiOutlineSomeconventionalresearchesAnovelmethodbasedonevaporativecoolingandsubsequentwater-droplettemplatingApplicationTemplatingmethodsbasedonself-assemblytechniquesSelf-assemblyofcolloidalmicrospheresonasolidsubstratetoforma2DcrystallinestructureasatemplateInfusionofothermaterialsintothevoidsoftheself-assembledmicrospheressolidificationofthematerialRemovalofthetemplatemicrospheresProblemsDifficulttoself-assembletemplatemicrosphereswithlong-rangeorderUnstableEasilydamagedduringtheremovalthetemplateTheFlowChartoftheNewMethodOpticalimagesofpolystyrene/silicafilmswithorderedarraysofpores:a)lowresolutionb)highresolution

SEMimages

withdifferentporesizes

HowtocontrolporesizesTheamountofTEOSPolystyrenebeadsizesTheamountofthesolutionaddedontothesurfaceofthesubstrateHowtocontrolporesizesTherelationshipsbetweentheporesize,polystyrenebeadsizeandamountofTEOSadded.

PatterningofPSMicrobeadsonthePorousFilmsAsuspensionofpolystyrenemicrobeadswasdroppedontotheporousfilm,fullycoveringthetopsurfaceofthefilm.Thefilmwasthenshakenonashakertoallowasmanybeadstogointotheporesaspossible.Thesurfaceofthefilmwasthenwashedwithwatertoremovetheexcessbeads.Theexcesssolutionwasthenallowedtoevaporateatroomtemperature.ThesizeinfluenceinpatterningthemicrobeadsWhenthesizeofthemicrobeadsisbiggerthanthatofthepores,themicrobeadssitontheporesratherthanenteringthemWhenthesizeofthemicrobeadsissimilarto,orabitsmallerthan,thatoftheporesthemicrobeadscanentertheporesa,b)Opticalandfluorescenceimagesshowingthepatterningoffluorescentpolystyrenemicrobeadsontheporousfilm.ConclusionItisanonlithographicbreath-figuremethodItcanbeachievedonthepolymerfilmsThesizeoftheporescanbereadilycontrolledbycontrollingthesyntheticconditionsApplicationThismethodhasthepotentialtobeusedinbead-basedmultiplexeddetectionandsensingapplications.Patterningofprotein-immobilizedpolystyrenemicrobeadsonthefilmwasconductedusingfluoresceinisothiocyanate-conjugatedbovineserumalbumin(FITC-BSA)ReferenceB.François,O.Pitois,J.François,Adv.Mater.1995,7,1041.J.Peng,Y.C.Han,Y.M.Yang,B.Y.Li,Polymer2004,45,447.G.Widawski,M.Rawiso,B.François,Nature1994,369,387.O.Karthaus,N.Maruyama,X.Cieren,M.Shimomura,H.Hasegawa,T.Hashimoto,Langmuir2000,16,6071.Thankyou!人有了知识，就会具备各种