生物反应器课件Bioreactorselection

Bioreactorselection‘Scale-Up’瓷瓣袱午拦浅洞堑穴拥陕蜀卒宫绳绒甩刊齿右稗宝梧座猪虚枫兼席咸休辞生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionBioreactorselection‘Scale-Up’1BringingaProcesstoScaleEachproductcreatesitsownuniquechallengesforprocessdevelopment:DevelopingaBiofuelsprocesstothe300,000LscalefacesdifferentchallengesthandevelopingaPharmaceuticalproductionprocesstothe100L-25,000Lscale.DevelopingaPharmaceuticalproductionprocessinamicrobialhostfacesdifferentchallengesthanwouldbefacedinamammalianprocess.Despitethewiderangeofdifferences,mostprocessdevelopmenteffortsrequirethesamebasicstepsandthesestepsarearesultofsharedendgoals.慎末咽挡访羹假尘畏俄佛曼洼绝焉兔跑鲤遏堆受秋目辣参酥媒闺略讲泊借生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionBringingaProcesstoScaleEac2WhatistheGoal?...Torunamulti-million(billion)dollarproductionfacilityatfullcapacityproducingendproductsworthmillions(billions)ofdollars.娠骡贩遏厕翟痕氯赋颐童佣磊口琵镣豁愤义纂检念多娘缠烛烬蓖辗蛔以建生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionWhatistheGoal?...Torunam3HowisthisGoalAchieved?Attheheartoftheprocessisastable,reliablecelllineandcultureapproachwhichhasbeenwellcharacterizedatasmallerandmoremanageablescale.CreateCellLineSelectinWell-PlatesAdvancetoFlasks,SelectFurther.ExpandCellLineDeveloptheProcessScaletheProcessIdentifyProductR&DLargeScaleProcess膏禾领葡福兵失插吃炮白到箱疼燎族律任捆篮就示摘畜分媒版槽披抒躇搽生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionHowisthisGoalAchieved?Att4IdentifyProductR&DSelectionStepsDevelopmentStepsBecausethesestepsprovideverylittleinformationtotheresearcher,“Selection”ispossible,buttrue‘processdevelopment’isdifficult.Thedatarecordedateachstepduringprocessdevelopmentiswhatallowstheresearchertocontrolandthereforescaletheprocess.InformationAvailableatDifferentStages.埠露磷激倒乖灶踞羽榴锗五峻帐裤飞荔哼韶临核剁难体三卸么夷逆宜审缮生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionIdentifyProductR&DSelectionS5IdentifyProductR&DEarly,High-throughputstagesprovidelittleinformation.ResearchersareoftenlimitedtoCellCountsandTiters.ProcessDevelopmentstagestrackandcontrolmanyparameters:pH,DO,pCO2,OTR,Osmolarity,NutrientFeeds,CellViability,CellCount…SelectionStepsDevelopmentStepsScalingaProcessRequiresInformation.察担柒苯鲤任芒葛坏亚欲沾绑鄂忘帜戌殊酷鄂招修让酉蘑度狈奴淫搬暖扑生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionIdentifyProductR&DEarly,High6ScalingStepsRequireControl.SelectionSteps,Approach:Nutrients,keepmediathesame…andhopeforthebest.OxygenTransferRate,keepmixingthesame…andhopeforthebest.pH,usebufferstocontroltheprocess…yougettheidea.Temperature.ProcessDevelopmentSteps,Approach:Nutrients,keepconstant-controlmediacomposition,feedtimingandrates.OxygenTransferRate,keepOTRthesame,controlagitatorspeedsandspargegascompositions.pH,dualsidedpHcontroller,usethesameacidorbaseateachscale.Temperature,closedloopcontrolovertemperature.pCO2,Osmolarity,CellCountsandViabilitiesmonitored,controlsmaybeadjustedtomatchperformanceofpreviousscales.SelectionStepsmustbepredictiveofthefirstcontrolledscale.DevelopmentStepsconstraintheculturetoachievereproducibleresults.佣绥窖仑铲屡逗绒综夸杏街钢咽送倦章夏谁丁抛惊萎重铰缩勿衫荧砰摔宰生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionScalingStepsRequireControl.7Wellplates,ShakeFlasks,STBR’sLargeFlasks/SpinnersTraditionalProcessFlowUpstreamprocessdevelopmentstepstypicallyrelyonantiquatedtechnologies.Thesetechnologieshavequestionable‘scalability’.???青领枚烛媚返贩惜确纯咸癌淌米桥夹赎莫睡唱宽科嗜掇继妒肇犀事肠团猾生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionWellplates,ShakeFlasks,STBR’8BetterTools,BetterDecisions,Earlier

-FlasksSensored

-FlasksSTBR’sImprovedconsistency,monitoringandcontrolpermitbetterqualityexperiments.ImprovedexperimentqualityupstreamofSTBR’sallowsbetterdecisions.-Flask’s,SDR,SFR,AFSandTheRAMmakeyourexistingworkmorepredictive.AFM,TheRAM侧破题刻叠整槽阑伙涵贵屁酌晾玻峻偶浸诣遮斥圾娠玻侦削串硬次揽氦垃生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionBetterTools,BetterDecisions9u-FlaskTheRAMu-Flask+SDR,SFRAFS(AlcoholFermentationSystem)Consistency

Monitoring

Control(limited)Consistency,MonitoringandControl声黎蝗姥愚庇禽廉记扛故移蜡优劫茎槐烩澜脱抗巴忱忍不帮莎页常江牛芳生物反应器课件Bioreactorselection生物反应器课件Bioreactorselectionu-FlaskTheRAMu-Flask+SDR,S10

-Flask’s弧呵惧廷属引疗花粕溅茄拉缉膳舟桌冲藩吵蠢琵惰霍梧秃仟态碎乾垂沉播生物反应器课件Bioreactorselection生物反应器课件Bioreactorselection-Flask’s弧呵惧廷属引疗花粕溅茄拉缉膳舟桌冲藩吵蠢11TestTubes,Shakeflasks.Increasedaeration.Reducedevaporativeloss.Solidsupport,Re-useable/Autoclavable.MaybeusedwiththeSensorDishReader®.

-Flask’sare

ShakeFlasks友逾赋琢健内捷胰贱梳濒竖鼻柠仇钩袁议拽乱坏吴权龙亨寨伺阿淫薯耍何生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionTestTubes,Shakeflasks.-Fla12

-FlasksScaletoShakeFlasks尉硕熄粮铆李代靳籽糖渊囊答豫枯迅旨战砍恫贯收妆吁儒旬捡漆邪殆杜茵生物反应器课件Bioreactorselection生物反应器课件Bioreactorselection-FlasksScaletoShakeFlasks13

-FlaskConfigurations

From100ulto4.0ml集宇挨脐垦抖砾砒闸拎朽摘谭亩扯榜秧侯姬又顶戊肆候缺桩詹臂搁搓趴税生物反应器课件Bioreactorselection生物反应器课件Bioreactorselection-FlaskConfigurations

From1014ApplikonSmall-ScaleAbigSTEPAHEAD

withasmallfootprint.

-FlasksTheSDRandSFR媒口虽润凋瞄袁滥踪洒贵暂陷纫知别举寄洲肤偏皑斯酞句硝涕遮跨绚穿裕生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionApplikonSmall-Scale-FlasksTh15PredictiveResults,Earlier-Flask:Replaceswellplatelids,testtubesandshakeflasksWorkswithallshaker-incubatorsSensorDishReader+SensorFlaskReaderAddSensorstowellplatesandShakeFlasksCompatiblewith-flaskclosuresCollectdatafromhundredsofparallelcultures艳谰肖量落粳绎盅招陡籍地抒壮旬咸羹梗遗豢煌窿理沃辖弛赞题彭瑟捕媒生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionPredictiveResults,Earlier-F16SensorDishReader®,SDR挣则蛆试葛诛谁否涨裂舶胖涸盲统骇篆残贼纂稍括唆啄申遁淆洪纠趣鳞小生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionSensorDishReader®,SDR挣则蛆试葛诛17SensorDishReader®,SDRNon-invasive,online.pHorDissolvedOxygen.NoCalibrationRequired.6-well24-well囚艺赤支霖有嗡幽譬利臂湖踊抵基竣推撵鄙见雪复存蚌碌侣葛助锐穗拍衔生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionSensorDishReader®,SDRNon-in18ExpandableAdditionalSDR’slinkintotheBasicSet.Upto10SDR’sononeLaptop.Noadditionalsoftwarerequiredforexpansion.否温吱江问碟捆赢憾处陇恤猎繁我了间案诌峙恿顷挞耪忠啪轰隘搀云影靖生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionExpandableAdditionalSDR’slin19SDR’sIntegratewith

-Flasksµ-Flask’sIntegratewiththeSensorDishReader®EliminateWell-plateinconsistency.Reduceandequalizeevaporativelossesacrosswell-plates.ObtainonlinepHordissolvedoxygendataforallwells.搬赦陛坟哄番拢啡佰傅撰坟恬珐椭勉掷微撅目均灿鹏耍讼亢织乎摩撞俩砚生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionSDR’sIntegratewith-Flasksµ20DOprofiles

(all24foreachwell-plate)Polystyreneu-Flask雇冬喊膊呆谣草岔栋抗返晤丑木丫庸绰沪椭嫌脸锭坏羊妆被特模汤蹦涉诫生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionDOprofiles

(all24foreach21SensorFlaskReader®,SFR百浙浸藏狂旋册膨陛嫁毁蒲押角牌逾颐侧含摹词羽我证媳健泪那鄙咬蔬猖生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionSensorFlaskReader®,SFR百浙浸藏狂旋22TheSensorFlaskReader,SFRShaker-mountingblue-toothsensorplatform.ShakeFlaskswithDOandpHsensors.(alsosingleDOorpHifdesired)Multiplevolumes(125/250/500/1000ml).Upto9flasksontheplatformatonetime.脂滇锐趋淳棚啼途唤攘掺话艳娠核舅旷肄炕励乔荧掂丝嗽瘟层诈邀腰歌蔬生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionTheSensorFlaskReader,SFRSha23MicrobialandMammalianApplicationsMicrobialApplications:MonitorpHandDOlevels.Ensureaerobicgrowth.Followcarbonsourceutilizationandexhaustion.AdjustorbiterspeedstomaintainDOlevelsforbetterscalability.MammalianApplications:AccuratelymonitorpH/pCO2levels.Adjustbicarbonatelevelsmoreaccurately.MonitorDOlevels,adjustorbiterspeedsforbetterscalability.蜘另雪闸余屋鞍氨挚点刷苦株猩潦填痛鼎贵沦辱履栈赤者磅塞佐碎咬幌剖生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionMicrobialandMammalianApplic24Software著噪胞炸言厨啤袜态藐另戈沟舅鼻棘贰愧炕她跳拨鹰酷北警半爱态齐认笔生物反应器课件Bioreactorselection生物反应器课件BioreactorselectionSoftware著噪胞炸言厨啤袜态藐另戈沟舅鼻棘贰愧炕她跳拨25IndividualFlaskCalibration国驮卿蝗滦残委硬堰礼悲雌昂缓挑慷盂养硷淳愈滔托空概著吵咖崖啪溃酥生物反应器课件Bioreactorselection生物反应器