编制说明-腹泻粪便标本中溶藻弧菌检验

《腹泻粪便标本中溶藻弧菌检验》编制说明1依据《北京预防医学会团体标准管理办法(2023年版)》，按照《关于开展疾病预防控制中心向北京预防医学会标准化管理办公室提出申请，2023年3月2版本、征求意见稿、送审稿、报批稿的核定，本、征求意见稿、送审稿、报批稿的核定，王建国：方法技术指标的确定及送审稿、报3败血症、中耳炎等疾病。超过20种弧菌可引法国比斯开湾一份关于2001-2019年弧菌感染的临床流行病学调查报告显示溶种病理有关，病死率为7.14%。我国海近些年在腹泻疫情的处置中会发现一些临床表现与副溶血性弧菌引起的疾（1）国外的标准都是针对弧菌属，尤其对副溶血性弧菌、霍乱弧菌、创伤②弧菌检验的国家标准有副溶血性弧菌和创伤弧菌③弧菌属的检验方法多是十几年前发布的，没有引入分子生物学、④仅有的溶藻弧菌检验标准是出入境机构关于出口食品中溶藻弧菌的检验4[1]王海波,张京云,王多春等.溶藻弧菌TaqMan实时PCR快速检测体系的建立[J].中国卫生检验杂志,2011,21(05)[2]GB4789.7-2013食品安全国家标准食品微生物学检验副溶血性弧菌主要技术内容在增菌培养基、选择性分离培养基、鉴定方筛的条件摸索方面，综合考虑溶藻弧菌及各种肠道致病菌在培养基中的生长表现，最后选定3%氯化钠碱性蛋白胨水为溶藻弧菌5中培养过夜，转种于3%氯化钠胰蛋白胨大豆琼脂，过夜培养。分别用VITEK系生化结果：30个菌株VITEK细菌生化鉴定系统给出的拉丁文鉴定结果均为萄球菌CICC23656、金葡201716MALDI-TOFMS验证结果：志贺CFU)。用溶藻弧菌质控球调制高浓度菌液1CFU/mL～10CFU/mL,低浓度菌液5份；将低浓度菌液1mL加入一份加了干扰菌的粪便标本，制作40份；其他5别标本号增菌液荧光PCR初筛结果鉴定结果Ct值生化结果（VITEK报告拉丁文名称）生化结果可信度（%）质谱结果(拉丁文名称)质谱评分荧光PCR结果性/阳性)荧光PCR（Ct值）高浓度1237453低浓度67N/A//////89N/A//////N/A//////N/A//////N/A////////////8N/A//////N/A//////N/A//////对照组N/A//////N/A//////N/A//////N/A//////N/A//////9algalgiolytalgiolytic浓度类别标本号增菌液荧光PCR初筛结果鉴定结果阴性/Ct值生化结果（VITEK报告拉丁文名称）生化结果可信质谱结果(拉丁文名称)质谱评分（布鲁荧光PCR结/阳性)荧光值）高浓度114.43Vibrioalginolyt99%Vibrioalginolytic811.21214.14Vibrioalginolyt99%Vibrioalginolytic211.35314.08Vibrioalginolyt98%Vibrioalginolytic311.20414.31Vibrioalginolyt99%Vibrioalginolytic311.47514.24Vibrioalginolyt99%Vibrioalginolytic2.07012.41低浓度614.13Vibrioalginolyt99%Vibrioalginolytic412.41714.16Vibrioalginolyt97%Vibrioalginolytic411.10814.60Vibrioalginolyt98%Vibrioalginolytic013.10914.68Vibrioalginolyt96%Vibrioalginolytic511.7614.08Vibrioalginolyt99%Vibrioalginolytic312.2914.38Vibrioalginolyt99%Vibrioalginolytic911.8814.51Vibrioalginolyt90%Vibrioalginolytic511.0215.05Vibrioalginolyt99%Vibrioalginolytic811.8114.17Vibrioalginolyt99%Vibrioalginolytic511.3314.78Vibrioalginolyt98%Vibrioalginolytic812.2315.13Vibrioalginolyt99%Vibrioalginolytic011.6415.27Vibrioalginolyt99%Vibrioalginolytic411.4514.79Vibrion97%Vibrion812.2014.99Vibrioalginolyt93%Vibrioalginolytic112.3914.97Vibrioalginolyt98%Vibrioalginolytic013.4415.13Vibrioalginolyt99%Vibrioalginolytic812.71N/A//////15.20Vibrioalginolyt99%Vibrioalginolytic411.9915.02Vibrioalginolyt99%Vibrioalginolytic610.7015.22Vibrioalginolyt99%Vibrioalginolytic2.00813.3415.26Vibrioalginolyt99%Vibrioalginolytic411.2514.33Vibrioalginolyt99%Vibrioalginolytic311.3214.59Vibrioalginolyt99%Vibrioalginolytic012.3114.79Vibrioalginolyt98%Vibrioalginolytic2.17611.8213.44Vibrioalginolyt98%Vibrioalginolytic210.6214.21Vibrioalginolyt98%Vibrioalginolytic312.0714.48Vibrioalginolyt99%Vibrioalginolytic711.9714.65Vibrioalginolyt99%Vibrioalginolytic811.3014.06Vibrioalginolyt96%Vibrioalginolytic611.8514.36Vibrioalginolyt99%Vibrioalginolytic511.7814.23Vibrioalginolyt99%Vibrioalginolytic813.0714.48Vibrioalginolyt99%Vibrioalginolytic512.2513.58Vibrioalginolyt99%Vibrioalginolytic2.03412.0814.43Vibrioalginolyt99%Vibrioalginolytic011.684014.73Vibrioalginolyt99%Vibrioalginolytic2.01511.5641N/AVibrioalginolyt99%Vibrioalginolytic112.124214.79Vibrioalginolyt98%Vibrioalginolytic712.534314.43Vibrioalginolyt99%Vibrioalginolytic611.934414.31Vibrioalginolyt99%Vibrioalginolytic411.534514.74Vibrioalginolyt99%Vibrioalginolytic812.96对照组46N/A//////47N/A//////48N/A//////49N/A//////N/A//////浓度类别标本号增菌液荧光PCR初筛结果鉴定结果/阳性Ct值生化结果（VITEK报告拉丁文名称）生化结果可信度质谱结果称)质谱评分鲁荧光PCR结果（阴性/阳性)荧光值）高浓度114.33Vibrioalginolyti99%Vibrioalginolyt2.29912.51214.12Vibrioalginolyti99%Vibrioalginolyt2.24611.65314.07Vibrioalginolyti96%Vibrioalginolyt2.30811.45414.56Vibrioalginolyti95%Vibrioalginolyt2.26712.41514.14Vibrioalginolyti90%Vibrioalginolyt2.46611.23614.65Vibrioalginolyti96%Vibrioalginolyt2.21511.46714.84Vibrioalginolyti90%Vibrioalginolyt2.21112.40814.68Vibrioalginolyti99%Vibrioalginolyt2.37011.209N/A//////14.65Vibrioalginolyti99%Vibrioalginolyt2.44511.39N/A//////15.53Vibrioalginolyti99%Vibrioalginolyt2.37312.1215.99Vibrioalginolyti99%Vibrioalginolyt2.36012.11N/A//////15.18Vibrioalginolyti99%Vibrioalginolyt2.32911.6515.45Vibrioalginolyti98%Vibrioalginolyt2.30711.4415.17Vibrioalginolyti96%Vibrioalginolyt2.28611.21N/A//////15.33Vibrioalginolyti99%Vibrioalginolyt2.31211.9315.26Vibrioalginolyti99%Vibrioalginolyt2.40712.1515.43Vibrioalginolyti96%Vibrioalginolyt2.19711.73N/A//////15.23Vibrioalginolyti96%Vibrioalginolyt2.37212.14N/A//////15.29Vibrioalginolyti96%Vibrioalginolyt2.37711.7515.66Vibrioalginolyti96%Vibrioalginolyt2.32511.47N/A//////N/A//////14.77Vibrioalginolyti96%Vibrioalginolyt2.33311.3315.42Vibrioalginolyti99%Vibrioalginolyt2.31111.2615.61Vibrioalginolyti95%Vibrioalginolyt2.25510.9915.12Vibrioalginolyti99%Vibrioalginolyt2.41510.9815.98Vibrioalginolyti96%Vibrioalginolyt2.36712.0015.14Vibrioalginolyti96%Vibrioalginolyt2.23011.7414.99Vibrioalginolyti97%Vibrioalginolyt2.35512.01N/A//////15.18Vibrioalginolyti96%Vibrioalginolyt2.28011.8515.28Vibrioalginolyti99%Vibrioalginolyt2.26711.9715.23Vibrioalginolyti99%Vibrioalginolyt2.27012.184015.13Vibrioalginolyti97%Vibrioalginolyt2.21210.9641N/A//////4215.19Vibrioalginolyti99%Vibrioalginolyt2.29911.7743N/A//////4414.31Vibrioalginolyti97%Vibrioalginolyt2.25612.034515.24Vibrioalginolyti97%Vibrioalginolyt2.35511.47对照组46N/A//////47N/A//////48N/A//////49N/A//////N/A//////浓度类别样本号增菌液荧光PCR初筛结果鉴定结果阴性性值）生化结果（VITEK报告拉丁文名称）生化结果可信度)质谱结果(拉丁文名称)质谱评分 安荧光PCR结果（阴性/阳性)荧光PCR（Ct值）高浓度1Vibrio93Vibrio9.02Vibrioalginolyticus94Vibrioalginolyticus9.2793Vibrioalginolyticus92Vibrioalginolyticus9.3034Vibrioalginolyticus93Vibrioalginolyticus9.3425Vibrioalginolyticus93Vibrioalginolyticus9.476低浓度626.3Vibrioalginolyticus93Vibrioalginolyticus9.3357///////8///////9Vibrio93Vibrio9.225.4Vibrioalginolyticus95Vibrioalginolyticus9.00829.1Vibrioalginolyticus93Vibrioalginolyticus9.268Vibrioalginolyticus93Vibrioalginolyticus9.426Vibrioalginolyticus93Vibrioalginolyticus9.34928.1Vibrioalginolyticus94Vibrioalginolyticus6228.9Vibrioalginolyticus93Vibrioalginolyticus78///////25.4Vibrioalginolyticus95VibrioalginolyticusVibrio93Vibrio9.226.4Vibrioalginolyticus93Vibrioalginolyticus9.0532028.3Vibrioalginolyticus93Vibrioalginolyticus752129.7Vibrioalginolyticus93Vibrioalginolyticus3022Vibrioalginolyticus93Vibrioalginolyticus9923Vibrioalginolyticus93Vibrioalginolyticus9.00224///////2526.4Vibrioalginolyticus93Vibrioalginolyticus2226///////2723.8Vibrioalginolyticus94Vibrioalginolyticus9.4762824.8Vibrioalginolyticus93Vibrioalginolyticus9.42529Vibrio93Vibrio9.53026.6Vibrioalginolyticus95Vibrioalginolyticus9.5313125.5Vibrioalginolyticus93Vibrioalginolyticus9.4423230.3Vibrioalginolyticus93Vibrioalginolyticus9.0023325.3Vibrioalginolyticus93Vibrioalginolyticus9.0343428.4Vibrioalginolyticus93Vibrioalginolyticus9