LncRNA全国讲座-课件

LncRNA全国讲座“Learner&Nontrivial&Confidence”-RNA全国技术中心技术LncRNA全国讲座“Youstartprojectsandyouhavenoideawhattoexpect.It’sthatkindofchallengethatIenjoyverymuchasascientist.”LncRNA全国讲座CircRes2012LncRNA全国讲座TherapeuticapproachInclinicaltrialsApprovedtherapy

EnzymereplacementtherapyYESYESGenetherapyYESYESNuclearreceptorligandsYESYESChromatinorDNAmodulatorsYESYESArtificialtranscriptionfactors YESNOMicroRNAmodulators YESNOCis-actinglncRNAmodulators NONOTrans-actinglncRNAmodulators NONO非タンパクコードRNAを利用した医薬開発GenomeNetworkProtein2Protein1Protein3Protein4Protein5Protein5PGene2ProteinXGene10ncRNA3ncRNA4Gene5Gene6DrugGene7Protein10Protein12Gene3Protein7Protein7Protein8Protein9Gene1Protein11Protein13ncRNA1ncRNA6Gene4Gene11Gene9ncRNA2Gene8DiseaseNewTargetofMedicineMutsumiKanamori-KatayamaPh.D.YoshihideHayashizakiM.D.,Ph.D.RIKENGenomeSciencesCenterGenomeExplorationResearchGroupLncRNA全国讲座LncRNA全国讲座LncRNA全国讲座Ryanet.al,BioEssays29:288–299(2007)LncRNA>200nt结构类似mRNA具有外显子/内含子低蛋白编码潜力LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座CabiliMN.IntegrativeannotationofhumanlargeintergenicnoncodingRNAsrevealsglobalpropertiesandspecificsubclasses.GenesDev.2011Sep15;25(18):1915-27LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座ncRNAPANDA募集转录因子NF-YA，启动细胞凋亡通路LncRNA全国讲座Cell2011ncRNA-andPc2Methylation-DependentGeneRelocationbetweenNuclearStructuresMediatesGeneActivationProgramsLncRNA全国讲座NatureMedicine2010ExpressionofanoncodingRNAiselevatedinAlzheimer’sdiseaseanddrivesrapidfeed-forwardregulationofβ-secretaseexpression早发老年性痴呆病中BACE1-AS高表达导致BACE1mRNA稳定性增高，蛋白积聚，体现了ncRNA的毒性效应BACE1BACE1-ASLncRNA全国讲座拷贝数染色体定位表达LncRNA全国讲座LncRNA全国讲座IF:31.027LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座芯片结果聚类图测序结果散点图backNorthernblotlncRNA-DC在免疫细胞中的表达情况lncRNA-DC在DC/mono细胞群中的表达情况backChIP-Seq与ChIP-PCR结果back荧光素酶报告分析，验证转录因子结合区域LncRNA全国讲座backLncRNA全国讲座干扰结果过表达结果backLncRNA全国讲座抑制DC细胞分化back通过STAT3的RIP实验验证LncRNA全国讲座Lnc-DC与STAT3相互结合back进一步验证Pulldown和RIP的结果LncRNA全国讲座back结果表明，lnc-DC的3′末端序列（265-417）与STAT3的C端结合（583-770）。STAT3的活化依赖C端Tyr705的磷酸化。LncRNA全国讲座back研究结果表明，S3I-201处理的细胞系与RNAi干扰后的细胞系在基因表达差异方面非常相似。LncRNA全国讲座LncRNA全国讲座高通量检测生物信息分析表达、定位验证上游验证功能验证下游验证LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座Affymetrix®TranscriptomeAnalysisConsole2.0(TAC2.0)Software

LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座IF:10.143LncRNA全国讲座IF:4IF:11.139LncRNA全国讲座LncRNA全国讲座上游下游反义链正义链LncRNA全国讲座LncRNA全国讲座LncRNA全国讲座

Hepatology2011LncRNA全国讲座通过网络找到调控lncRNA最多的转录因子同时找到能被不同转录因子调控最多的lncRNA使用德国Biobase数据库商业版数据库LncRNA全国讲座LncRNA全国讲座AffymetrixPromoter1.0RArrayAffymetrixHTA2.0LncRNA全国讲座蓝色线表示两点之间的关系需要计算绿色线表示两点之间的关系通过转录因子的预测得到LncRNA全国讲座LncRNA全国讲座IF:38.597LncRNA全国讲座深灰色：激活浅灰色：抑制LncRNA全国讲座I