如何回复审稿人意见

DearEditor,

Wehavestudiedthevaluablecommentsfromyou,theassistanteditorandreviewerscarefully,andtriedourbesttorevisethemanuscript.Thepointtopointrespondstothereviewer’scommentsarelistedasfollowing:

Respondstothereviewer’scomments:

Reviewer1

Comment1:inpage3,line40,wefedrats..."changedtoratswerefedwith...

Response:Accordingtothereviewer’scomment,wehavecorrectedthesentence.Furthermore,wehavehadthemanuscriptpolishedwithaprofessionalassistanceinwriting.

Comment2:page25.Thestyleofreference40isnotright(usinginitialsforthefirstnames).Sincethispaperhasbeenpublished,thevolumeandpageNosshouldbeprovided.

Response:Thankyouforyourcarefulwork.Wehaveaddedthevolumeandpagenumbersforreference40.

Reviewer2

Comment:Iwouldliketothanktheauthorsfortheireffortsinaddressingthecriticismswithadditionalexperiments.Theonecriticismthattheydidnotaddresswasrelatingtoenergyexpenditureasthereasonthattheanimalsonthelowcalciumdietgainedmoreweight.WhileIunderstandthatperformingthisexperimentwillnotaffecttheconclusionofthismanuscript,IdobelievethatthispointcouldbediscussedintheDiscussionsection.

Response:Thankyouforyourvaluableadvice.Basedonthepreviousrevision,wefurtheraddresstherelationshipbetweenlowcalciumdietandenergyexpenditureinthesectionofdiscussionaccordingtoyourthoughtfulcomments.

Reviewer3

Comment1:Inthetextyouoftenwrite:“Aspreviouslydescribed”.Unlessthatpaperisfromyourlaboroneofthemethodpaperco-authorsisonthepresentMSthisisnotquitepropersincethestatementinfersmethoddevelopmentfromyourlab.Therearenumerousinstanceslikethatinthemethodssection;theseshouldallbechanged“accordingtothosedescribedby…..”

Response:Wearesorryforthislanguagemistake.Wehavecarefullycorrectedthisphrasethroughoutthemanuscriptaccordingtoyourcomment.

Comment2:Therearestillsomewording,sentencestructureandgrammaticalissueseveninthisbasicallywellputtogetherMS.Forexample,whileauthorsmayhavebeenexcitedaboutthedatayoucannotstartasentencewith“Excitedly”inline418or“Whatever”inline395.

Response:Thankyouverymuchtopointoutthesentencestructureandgrammaticalissuesinourmanuscript.Accordingtothecommentsfromyouandtheeditors,wepolishedthemanuscriptwithaprofessionalassistanceinwriting,conscientiously.

Comment3:Inmyviewabigomissioninthisworkisignoringtheanabolicsideoflipidmetabolismaswellasthermogenesisissues.ForexampleallanimalsconsumedthesameamountoffeedbutwehadextrafatstorageinthelowCadietgroups.Sowheredidtheextraenergygo?Zemeletal(citation34)insimilarworkindicatethatincreasedthermogenesisonthehighCadietexplainsthedissipationofdietaryenergy.FurthereventhoughZemeletal(#34)indicatedlipogenesiswasenhancedinthelowCadietsthatwasin2000andyoushouldhavemonitoredexpressionofFASandUCPeitherasmRNAabundanceoractualFAS/UCPchangesviaproteomicsorblottingtechniques.InanycasethesecontrolsaremissinghereandnotemphasizedintheMS.CasualreadingofthispaperwouldleadtotheconclusionthatthedietaryCaeffectonfatdepositionisstrictlyafunctionofincreasedordecreasedlipolysis.Whilelipolysisappearstobeamajorplayer,lipogenesisandthermogenesiscannotbeignoredforcompleteness.InFig8youalsoshowadeclineincAMPforthelowCadiet.WellbetaagonistsorcAMPenhancersregulatetranscriptionofadiposeandliverFAS(inrats(JBiolChem271:2307,1996)andrecentlywithlargeanimalmodels(HausmanetalJAnimalScience87:1218,2009andHalseyetalJAnimalScience89:1011,2011).Inaddition

Comment7:Line150-153.TheqRT-PCRmethodologyisnotatallunderstandableasyouciteaTexasA&Mpublishedpaper.ThisiscompletelyinsufficientwiththenewlyestablishedstandardsongeneexpressionviaqRT-PCR.Thereisnomentionofefficienciesofamplificationsinthesedatanorhowtheuseofthereferencegenewasestablishedetc.IthinkPfafflandBustinhaverecentlywrittenanarticleonthis;pleasetotallyrevise150-153inlinewithwhatyoudidandapplyingthenewstandards.

Response:Thankyouverymuch.BecausetheJNrestrictsthenumberoftotalwordsofmanuscript,wecitedtheTexasA&Mpublishedpaper.Inthenewlysubmittedmanuscript,wedescribethedetailedprotocolsinourlab.

Comment8:Line179onNotclearasinsentencestalkaboutdifferentATcellsourcesetc..revise.

Response:Wearesorryfornotaddressingtheadiposetissuecellsourcesclearly.Wehaverewrittenthemethods.

Comment9:AnypreviousdocumentableworkwithsiRNA?

Response:Yes,wehavedocumentableworkwithsiRNAinourresearchteam.TheresultswerepublishedinthejournalofBiochemBiop