MPM-1-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEMPM-1Cat.No.:HY-151503分⼦式:C₃₄H₄₄F₆N₄O₇分⼦量:734.73作⽤靶点:Autophagy作⽤通路:Autophagy储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性MPM-1海洋⽣物Eusynstyelamides模拟物，⼀种有效的抗癌剂。MPM-1可通过诱导坏死样死亡，在体外快速杀死癌细胞。MPM-1具有诱导免疫原性细胞死亡的能⼒。MPM-1在癌细胞中⼲扰细胞⾃噬(autophagy)和溶酶体膨胀。IC50&TargetAutophagy[1]体外研究MPM-1(0-50μM;4h)hascytotoxicityagainstvarioushumancancercelllines[1].MPM-1(8.5and17.0μM;1-6h)causesperturbationofautophagyandlysosomalswelling[1].MPM-1inducesthereleaseandexposureofdamage-associatedmolecularpatterns(DAMPs)relatedtoimmunogeniccelldeath[1].CellCytotoxicityAssay[1]CellLine:Jurkat,Ramos,HSC-3,MCF-7,A375,etal.Concentration:0-50μMIncubationTime:4hResult:ExhibitedpotentcytotoxicityagainstJurkat,Ramos,HSC-3,MCF-7,A375withIC50sof6.62ꢀ±ꢀ1.60μM,7.53ꢀ±ꢀ2.01μM,8.53ꢀ±ꢀ0.57μM,14.06ꢀ±ꢀ2.71μM,14.52ꢀ±ꢀ0.22μM,respectively.Immunofluorescence[1]1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECellLine:HSC-3Concentration:8.5and17.0μMIncubationTime:1,2,4and6hResult:Significantlyincreasedthetotalnumberofautophagymarkersp62(substrateofautophagy)andLC3B.Causedthedistributionofthelysotrackerdyemorediffuseandlessintense,indicatingthatlysosomalmorphologywasinfluenced.WesternBlotAnalysis[1]CellLine:RamosandHSC-3Concentration:8.5μMforRomasand17.0μMforHSC-3IncubationTime:0.5,1,2,3and4hResult:Increasedthereleaseofhighmobilitygroupbox1(HMGB1)fromcells.REFERENCES[1].SusannahvonHofsten,etal.ThemarinenaturalproductmimicMPM-1iscytolyticandinducesDAMPreleasefromhumancancercelllines.SciRep.2022Sep16;12(1):15586.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3