AgBr-NP@CTMAB抗白色念珠菌活性及在PMMA相关应用研究

AgBr-NP@CTMAB抗白色念珠菌活性及在PMMA相关应用研究AgBr-NP@CTMAB抗白色念珠菌活性及在PMMA相关应用研究

摘要：

随着医疗科技的进步和人们生活水平的提高，人类对高品质牙齿修复材料的需求也越来越大。然而，缺陷材料可能会导致口腔疾病的发生和发展。白色念珠菌是一种常见的口腔真菌病原，对PMMA修复材料具有可怕的生物附着性和异味生成能力。因此，开发一种安全高效的AgBr纳米颗粒@CTMAB修复材料已成为目前研究的热点。本文利用溶液法制备了不同质量比AgBr-NP@CTMAB纳米颗粒，并研究了它们对不同白色念珠菌菌株生长的抑制作用。结果表明，AgBr-NP@CTMAB抗白色念珠菌活性与CTMAB浓度密切相关，并与AgBr纳米颗粒的质量比有关。此外，在搭配使用AgBr-NP@CTMAB与PMMA修复材料后，材料的生物附着性和异味生成能力均不断降低，其力学性能略有下降，但仍能够满足需要。因此，AgBr-NP@CTMAB修复材料具有很好的应用前景。

关键词：白色念珠菌；AgBr-NP@CTMAB；PMMA修复材料；生物附着性；异味生成；力学性能

AgBr-NP@CTMABAntifungalActivityagainstCandidaalbicansandItsApplicationinPMMAMaterials

Abstract:

Withtheprogressofmedicaltechnologyandtheimprovementofpeople'slivingstandards,thedemandforhigh-qualitydentalrestorativematerialsisincreasing.However,defectivematerialsmayleadtotheoccurrenceanddevelopmentoforaldiseases.Candidaalbicansisacommonoralfungalpathogenthathasterriblebio-adhesiveandodor-generatingcapabilitiesonPMMArestorativematerials.Therefore,developingasafeandefficientAgBrnanocrystals@CTMABrestorativematerialhasbecomethecurrentresearchhotspot.Inthispaper,AgBr-NP@CTMABnanocrystalswithdifferentmassratioswerepreparedbysolutionmethod,andtheirinhibitoryeffectsonthegrowthofdifferentCandidaalbicansstrainswerestudied.TheresultsshowedthattheantifungalactivityofAgBr-NP@CTMABwascloselyrelatedtotheconcentrationofCTMABandthemassratioofAgBrnanocrystals.Inaddition,afterusingAgBr-NP@CTMABincombinationwithPMMArestorativematerials,thebio-adhesiveandodor-generatingcapabilitiesofthematerialscontinuedtodecrease,andtheirmechanicalpropertiesslightlydecreasedbutstillmettherequirements.Therefore,AgBr-NP@CTMABrestorativematerialhasagoodapplicationprospect.

Keywords:Candidaalbicans;AgBr-NP@CTMAB;PMMArestorativematerial;bio-adhesive;odorgeneration;mechanicalpropertiesThebio-adhesivepropertiesofdentalrestorativematerialscanleadtotheformationofbiofilmsonthesurface,whichcancauseadverseeffectsonoralhealth.Candidaalbicansisoneofthemostcommonmicroorganismsfoundindentalbiofilmsandisassociatedwithoraldiseasessuchascandidiasis.Toaddressthisissue,AgBr-NP@CTMABhasbeenproposedasapotentialsolutiontoreducebio-adhesivepropertiesandodorgenerationofPMMArestorativematerials.

TheadditionofAgBr-NP@CTMABtoPMMArestorativematerialssignificantlyreducedthebio-adhesivepropertiesofthematerials.ThemechanismbehindthisphenomenonisthatthepositivelychargedCTMABmoleculesonthesurfaceofAgBr-NP@CTMABcaninteractwiththenegativelychargedcellwallsofmicroorganisms,includingCandidaalbicans,topreventtheiradhesiontothematerialsurface.TheAgBrnanocrystalsinAgBr-NP@CTMABalsohaveantimicrobialpropertiesthatcaninhibitthegrowthofmicroorganisms.

Inadditiontoreducingbio-adhesiveproperties,theuseofAgBr-NP@CTMABalsoreducedtheodorgenerationofPMMArestorativematerials.ThisisbecausetheantimicrobialpropertiesofAgBr-NP@CTMABcaninhibitthegrowthofodor-causingmicroorganisms.Thecombinationofthetwomechanismsleadstoareductionintheformationandaccumulationofbiofilmsonthesurfaceofthematerial,whichcanimproveoralhealth.

AlthoughtheadditionofAgBr-NP@CTMABslightlydecreasedthemechanicalpropertiesofPMMArestorativematerials,thematerialsstillmettherequirementsfordentalapplications.Therefore,AgBr-NP@CTMABrestorativematerialhasagoodapplicationprospectindentalpractice.Futureresearchcanfurtherexplorethelong-termantimicrobialefficacyandbiocompatibilityofAgBr-NP@CTMABrestorativematerialsInadditiontothepotentialbenefitsoutlinedabove,therearesomepotentialdrawbacksandlimitationsassociatedwiththeuseofAgBr-NP@CTMABrestorativematerialsindentalpractice.Oneimportantconsiderationisthepossibilityoftoxicityorotheradverseeffectsassociatedwiththeuseofsilvernanoparticles.Whilesilverhaswell-knownantimicrobialproperties,thereareconcernsaboutthepotentialforsilvertoaccumulateinthebodyovertimeandcauseharm.Somestudieshavesuggestedthatnanoparticlesmaybemoretoxicthanlargerparticlesofthesamematerial,duetotheirsmallsizeandincreasedsurfacearea.Therefore,itwillbeimportanttocarefullyevaluatethesafetyandtoxicityofAgBr-NP@CTMABrestorativematerialsbeforewidespreaduseindentalpractice.

AnotherpotentiallimitationofAgBr-NP@CTMABrestorativematerialsisthattheirantimicrobialpropertiesmaynotbeeffectiveagainstalltypesofbacteria.Whilesilverhasbeenshowntobeeffectiveagainstabroadrangeofmicroorganisms,somespeciesmaybemoreresistanttoitseffects.Inaddition,thereisthepossibilityofdevelopingbacterialresistancetosilverovertime,whichwouldlimititslong-termefficacy.

Finally,itisworthnotingthatAgBr-NP@CTMABrestorativematerialsarenotastandalonesolutionforimprovingoralhealth.Whiletheymayhelptopreventtheformationofbiofilmsondentalrestorations,theydonotaddressotherimportantfactorsthatcontributetooraldisease,suchaspoororalhygieneoradiethighinsugarandothercarbohydrates.Therefore,itwillbeimportanttocontinuetopromotegoodoralhealthpracticesalongsidetheuseofthesematerials.

Overall,thedevelopmentofAgBr-NP@CTMABrestorativematerialsrepresentsapromisingadvanceinthefieldofdentalmaterialsscience.ByincorporatingsilvernanoparticlesintoPMMArestorativematerials,itmaybepossibletoimprovetheirantimicrobialpropertiesandreducetheriskofinfectionandothercomplications.However,furtherresearchisneededtofullyevaluatethesafetyandefficacyofthesematerials,andtorefinetheirformulationandapplicationforoptimalclinicaluseInadditiontothepotentialbenefitsofAgBr-NP@CTMABrestorativematerials,therearealsosomechallengesandlimitationsthatneedtobeaddressed.Oneissueisthepossiblecytotoxicityandgenotoxicityofsilvernanoparticles,whichcanhavenegativeeffectsonhumancellsandtissues.AlthoughthereisevidencethattheAgBr-NP@CTMABmaterialshavelowtoxicityandbiocompatibility,morestudiesareneededtoconfirmtheirsafetyandlong-termeffectsonoralhealthandoverallhealth.

Anotherchallengeisthecomplexityandvariabilityofdentalrestorativeprocedures,whichcanaffectthepropertiesandperformanceofthematerialsused.Factorssuchasmoisture,temperature,pH,andmechanicalstresscaninfluencethebonding,durability,andaestheticsofdentalrestorations,andmayalsointeractwiththesilvernanoparticlesintheAgBr-NP@CTMABmaterials.Therefore,itisimportanttooptimizetheprocessingandhandlingofthesematerialstoensureconsistentandreliableoutcomes.

Furthermore,theclinicalapplicationsandindicationsofAgBr-NP@CTMABmaterialsmaydependonthespecificneedsandconditionsofdifferentpatientsanddentalcases.Whiletheantimicrobialpropertiesofsilvernanoparticlesaregenerallybeneficialforpreventingandtreatingdentalcariesandotherinfections,theymaynotbenecessaryorappropriateforalltypesofrestorationsorpatients.Insomecases,alternativematerialssuchascompositeresinsorceramicrestorationsmaybemoresuitableoraesthetic.

Overall,thedevelopmentandevaluationofAgBr-NP@CTMABrestorativematerialsisapromisingareaofresearchforimprovingtheperformanceandsafetyofdentalrestorations.Withfu