常用限制性内切酶酶切位点汇总

AhdI识别位点AhdI识别位点BaeI识别位点作编号作编号：GB8878185555334563BT9125XW/作者：凤呜大王*AatII识别位点5…GWGffc…h3J.CJGCAG..5JAcc65I识别位点5：..GTtMKAC...3J3J.CAKMJG.5-AccI识别位点5：..GTtMKAC...3^3J.CAKMJG.5-AciI识别位点5"…(fCGC...3r3…GGQp…5’AclI识别位点5",A^CGTI・・・3‘3J.TTGC/A...5^AcuI识别位点5：..CTGA..3"3；.,GACTTG{N}^...hAfeI识别位点..AGC^CT...3r3...TCGmCGA...5-AflII识别位点『…Ettaag…s3-...■$AA.rTjC...5^AflIII识别位点5：..X'CRYGI..3*3；TGYRC^A.5'AgeI识别位点对…TGGCG^A…5’AleI识别位点5-...CACMf/HNeTG..,.3r3：..GTGMI^NNCAC...5^AluI识别位点创3'...TGpA...5'AUlwI识别位点5；p.GGATC<N).t..T3：..CCTA.G^N)r'..一亍AlwNI识别位点5；,.C^GNNNtCTG,,.3^3J.,.GJG4NMNGAC,,J5JApaI识别位点5：,GGGC<?C3：..qpCGGG...5JApaLI识别位点护…厅TGCAC3J..CACG1；G…5’ApeKI识别位点5：,.GTCWCC,,.3^u.CGwqp…hApoI识别位点$JfTaattJ一击3J..YTTAAXR..5^AscI识别位点5...GG'tGCGCC,.,3^3J..CCGGGC^G.,5JAseI识别位点,ATFTAAT^.3*3.TAATJA..5JAsiSI识别位点Z.-GCGAT^GC..3r3\.CGCJaSCG..5^AvaI识别位点5,..CVCGRG,.33,.3GC車…hAvaII识别位点5'.,・(StWCC,,・M3J..CCWGi^..5JAvrII识别位点5：,T耳GG对…GGATqp…h5；,,GACNNhfrlNGTC.,.3^3：..CTGNI^NhNCAG...5^Bgii识别位点Bgii识别位点BsaWI识别位点BglIIBglII识别位点頁…代AT"33：..TCTA(^A..，5F『爲㈣A6N"丁人诂.33：kl£(N)-rG^LCATRG(N)?k.5rBamHI识别位点5,,.G^^TCC…h3.CGTAG^...5^BanI识别位点

BlpI识别位点5：..G(7tNAGC..3^3：.CGAN1；CG.5^5：.,<?GYRCC..・iT扩…■CCFlHQP…hBanII识别位点

Bme1580I识别位点5：.GKGCM'C..3*3J...-^MGGKG.5^•.石Ft石GfC-占扩…q/CGRG…hBbsI识别位点

BmgBI识别位点5\,.CA^rC...3r3...GTGJ3AG...5J5：..GAAG^CtN)fT...T3"..CTTCl5(M}；…亍BbvCI识别位点5：..CC^TCAGC...3*3；GGAG.CG…hBbvI识别位点5^..GCAGC^N^t..T3.CGTCG(M^BccI识别位点5：..CCATC(N)73-子,，.GQTAG叫..獻BceAI识别位点

BmrI识别位点5^rr,ACTGGG(N^,t..3*3\.TGACCC(N}4i.BmtI识别位点5；..GCTAG'C,-.3^3：.CJjATCG-.5"BpmI识别位点y...ctggag^m^J...y3：..GACCT匚厲比…亍Bpu10I识别位点5\..CCVnAGC."丁3J.GGAh7；CG...5^BpuEIBpuEI识别位点5；..CTTGAG(N)，7,..33：.AA<：TC(N)h^..BsaAI识别位点3J.,,RTGiCAY...5^BsaBI识别位点5；..GATNN^NATC-,3a^.^CTANhl^NlAO.5^BsaHI识别位点5；,.GFftGYC,,-3r31...0YGO^RG..5^BsaI识别位点5r...GGTCTCCN)/...T3..CCAGAG(M)^...5^BsaJI识别位点5\..CTCNMG<3..S'3J...GGNMC^..5^ACGGCtN^/.-3^3…TGCUG㈣％EBcgI识别位点5...^(NiCGAtN^TGC^N)/3BciVI识别位点忙一石TATCC[M)「…丁3.CATAGG.5^BclI识别位点S\..'rtsATCA..3-扩…ACTAGJ.,.5JBfaI识别位点5"..(?TAG...3r扩…GA"…XBfuAI识别位点5^...ACCTGC(N)7...3^Tr..TGGACG(N\...^5r..,GCCNNNN¥NGGC...3r3：..CGG^NNMNGCO..5JDrdI识别位点DrdI识别位点BtsCI识别位点BsmFIBsmFI识别位点5：..v/ccggw,.,y3J..WGGCC^W..BsaXI识别位点5：5：..v/ccggw,.,y3J..WGGCC^W..BsaXI识别位点5：.Te(M；AC(N),CTCC.3J3；..|?(N}T电(M}'PGAGG(N)T...5^BseRI识别位点5：.GAGGAG(N)，7.T3\..CTCCTC(N)..5^3\..CCCTG^N)tJi，,.5rBsmI识别位点5^,,,GAATGCMTr.3^3OTTAC^Il..5RBsoBI识别位点护，小GGRG「3…GRGfG…hBsp1286I识别位点5：..GCGCH'C,,.3^3r...qHCGDG..5rBspCNI识别位点创二.：二丁m、厂'■作编号：GB8878185555334563BT9125XW作者：凤呜大王*3'...GAGTC.5^作编号：GB8878185555334563BT9125XW作者：凤呜大王*BspDI识别位点h…ATtCGAT,n3^扩…TAGCJA...5"BspEI识别位点,TtCCGGA.-.3*BseYI识别位点3JBseYI识别位点3J.GGG7Cfi...5JBsgI识别位点5：..GTGCAG(N)1T3..CACG7C(M}UiSrBsiEI识别位点5P.-CGHY'fcG..y3；..GC^YRGC…5’BsiHKAI识别位点5；..GWGCWt…tr3J...(^WCGWG.5JBsiWI识别位点BspHI识别位点5r.,,rtaTGA,,.3^3：..AGTACJ…hBspMI识别位点5：..ACCrGC(N)J.r.T3"...TGGACG和忑.一SBspQI识别位点..GCTCTTC(N}1T113-3..CGAGAAGH(i.-5^BsrBI识别位点样…CGbtTG…歹扩…GGqGAG…引5；..C^TACG,,5；..C^TACG,,3^3」…右CATGaC..5^BslI识别位点5：..CCNNNMMVJNGG...3^3H■GGNN^NNNNNCC..5*BsmAI识别位点5：..GTCTC^hl)/.3CAGAG(M)>..BsmBI识别位点5\r.CSTCTC(N)/..3*3\.GCAGAG(N}5^..5^5；,,GCAATCNNT,n3^3J...GGTTAC^NN.5^BsrFI识别位点5；r,pwtCGGV,.3^3..YGGC<^R5JBsrGI识别位点5..ftTACA-3^3：,.ACATGJ…hBsrI识别位点5：ACTGGhT…3’3’…TGAC^CN..r5*

BssHII识别位点.BssHII识别位点.^CCCGC-..3^对…■CGCG^G.…弓BssKI识别位点5；.TCCNGG,.-3^3：..GGNCC^-.S1BssSI识别位点5：.-CTaCGAG…3一3’…GTOC^p…即BstAPI识别位点5；,.GCANNNhfNTGC3；.CGTN^NN忡N占GG…hBstBI识别位点5：.,TT'tGAA.-.3*3J.AAGCJT..5JBstEII识别位点5；,.^CTNACCr,・3‘厅…GCAhlTGfl...5,3：,.CGTCACJN.BtsI识别位点5；,nGCAGTQMNT,,3r3：,.CGTCAC^NN..5^Cac8I识别位点5：,.GCffNGC,,-3*3J..-CGi^NCG.5JClaI识别位点兰…aFcgat…丁3：..TAGCJA.S'CspCI识别位点5\.\11(N)CAA(N)jGTGG(N)i£.1J...3*3\.jS,13(N)G1T(N)1CACC(N^1V，.5*CviAII识别位点5；r.cTatq…丁3-.GTAJ：一hCviKI-1识别位点BstNI识别位点5\..C(?WGG...3r3..GG\A^CC-hBstUI识别位点5-.,GGtG,・33；.OC^SC...£BstXI识别位点5；..CCANNNNhTNTGG,,.,3*3：.GGTNaNhlhlNNACC.5’BstYI识别位点,RTGATCYr,.3^.YCTAt^R..5JBstZ17I识别位点頁.^T^TAQ,r.3^3J...CAl^ATG.,5^Bsu36I识别位点5..CtfTNAGG...3r3J...GGAN74CC.5^BtgI识别位点5：..(7CRYGG...3*3J.GGYRCjC.5JBtgZI识别位点5：..GCGATG^N^/,.3^&J.,.RGtC^...3j3&J.,.RGtC^...3j3J...Y€JlGFl..-5JCviQI识别位点3”…-5DdeI识别位点5\.<7TNAG.,3r3.GAhlTj^.5-DpnI识别位点5：GATC3^3：CTAG5^DpnII识别位点$J.^ATC.3\.GTAGa...5rDraI识别位点5；,.TTAA.3r.AAAJTT…5’DraIII识别位点.CAChNN'bTGk-,3*扩…GT琴忖NCAC.5；,,GACNiqItfjMNGTC.,,3^3...CTGNN^hlNNGAG...5^FseIFseI识别位点EaeI识别位点5：.・Y'GGGCR,..3*3J.RCCGG^..5JEaeI识别位点5...Y'GGCCR...3*3RCCG右yEagI识别位点5；.<TggCCG,3出…住CGGGp…£Earl识别位点忙一CTCTTC(M)「…丁3.GAGAAG(N)4^.5^EciI识别位点5；.GGCGGA^N)^..3^3：..CCGCCT(Nk4.,,5-EcoNI识别位点5；・，CCTMNVlNNAGG...3^3；..GGANNf^lNTCC...5^EcoO109I识别位点5,r■RG'tNCCY,,.3^3：..¥CCNG1GR...5JEcoP15I识别位点5：-CAGCAG(N)27.T3\..GTCGTcjN)a^...SREcoRI识别位点5.ATTC,-3^3J...GTTAAp...5^EcoRV识别位点扪…GATtTG■卫3：..CTAJAG...5^FatI识别位点h…tATG…孑3」…GTAQ…5’FauI识别位点5：..CCCGC(N)JL..3*3r,.,GGGCG..5rFnu4HI识别位点5；・，GC^NGC...3^3\CGNj^G.5*FokI识别位点阪一一GGATG(hl),.T.一3’3"..CCTAC㈣％_¥5\..GGCCG<3CC..,3r3...CCpGCCGG..,5JFspI识别位点扪…TG<rCGA扩…AC^CGT...5^作编号:GB8878185555334563BT9125XW/作者：凤呜大王*HaeII识别位点5：..RGCG<fy...y.^CGCGA..yHaeIIIHgaI识别位点..GACGC-(N^t3r.CTGCG^M},^HhaI识别位点5"……3r3...O^CG..5JHincII识别位点5,,.GTAC,,.3^3.GAFl^YTG.l.5jHindIII识别位点5,../CAGCTT,..3^3J.TTCGA^A.5^HinfI识别位点引…G訓NTC…3’3J,.CT忖AjG…HinPII识别位点VCGC...3*3J..■GGCJS...5JHpaI识别位点5：MSTfXAC…密対…CAAJTG…hHpaII识别位点5；,,(7cGG,..3*3J.-GGC^C..5JPmeIPmeI识别位点MmeIMmeI识别位点HphIHphI识别位点6...GGTGA(hl*…3一3.,GCACT(N)7i.5*Hpy188I识别位点..TcntcA…yTr.ACT…hHpy188III识别位点5：.TC^MNGA...3-3J.AGMN^T.5JHpy99I识别位点5；.rgginggT冷3：..^GCWGC..5^HpyAV识别位点h…CCTTC卅L・Sa.-.GGAAGtM^..5rHpyCH4III识别位点5"...ACN¥GT...3r3：一T^/tlCA…5’HpyCH4IVHpyCH4V识别位点亍…Tbs…;r3J.A<^GT...5JKasI识别位点5；,^GCGGC「33：..GCGCG^…hKpnI识别位点5,.,GGTACTC,..3r3J...qpATGG..5JMboI识别位点5；.,^ATC,..3r3」…CTAQ…hMboII识别位点5J,.GAAGA3\.CTTCTW),斗-hMfeI识别位点5：r,申ATTG…「3「3J...GTTAAaC...5JMluI识别位点5P-..^CGCGT,r.3^3：..TGCGC^A…hMlyi识别位点5.GAGTC{N]^.33：CTCAG(Mk..5^5...TCCRAC(N)2.t...3’3：.,AGGYTG(N)lt^.J5^MnlI识别位点5r.CCTC(W.38；irGGAQ<N^MscI识别位点5；,・TGGitCA…IT3J..ACCOST.5JMseI识别位点5r...TtTAA...3r3.AAV.5^MslI识别位点5：..€A¥NNtNNRTG...3*3r...CTRNNJ1NYAC.一.5「MspAII识别位点5；,CM<StKG,33…GKqpLflC…hMspI识别位点5；,rCTCGGr..^3：,.EGQp…宁MwoI识别位点5,..GCMNNNN^NMGC・歆….CGNN^NNNMNCG…一$NaeI识别位点5；,.GCtftGC,3J..-00^00…5’NarI识别位点y一GGtGGG33J.CCGqpG..5JNciI识别位点5rQCTSGG--3^3J...GG引CC…hNcoI识别位点扪,，氏ATGG,,3扩…GGTAQf…hNdeI识别位点5.CA^TATQ.3r3J..,GTA1；AC…5'NgoMIV识别位点5,■盈CGG©…孑歆.GGGCQP…hNheI识别位点5；,.dtTAGC,,-3^3J.CGATC^G…宁

Nlalll识别位点.CANlalll识别位点.CATTGt..3r3...^GTAC..5J3J..GAAAJTTG..5JPmlI识别位点NlaIV识别位点5；-G$曲CG…古3J...CCN^NGG.-.S^NmeAIII识别位点5：..GCCGAGfN^...^3：..CGGCTC(N)1fri^...5^NotI识别位点5：..GC^GCCGC...3*3J..CGCCGG^G..5JNruI识别位点5；,.TCfStGA,,,3^3…AGqpCT…5"NsiI识别位点5；..ATGC^T...3^3'...JAGG1■凸…hNspI识别位点5^..RCATtfY...3*a；\；GTACA..5^PacI识别位点5；..TTAAfVAA...3r时一AATJAATT...5-PaeR7I识别位点5：.,^TCGAG,.,3^3：.GAGCT^C…5’PciI识别位点5,..C^C^TG・・・3"3J.GTOj；AG...S*PpuMI识别位点5：,.RGTGWCCY,..3*3J.YCCWG^GR.5JPshAI识别位点5；,.GACNN^NNGTC,,卫'3：..CTGNN^NNCAG...5JPsiI识别位点5,TT^TAA...ff3.AAT^ATT...5FPspGI识别位点5.±G训GG…占3J,..GGWCC^.,5RPspOMI识别位点5,..^Tgggcc,,.T3’…CCCGQp…£PspXI识别位点5…VC^TCGAGB,,.T3：..BGAGCT^GV..,5^PstI识别位点5；,.CTGC^-G,..3■3J.C^ACGTC..5^PvuI识别位点S^-.CGAT^G...3'3r..GCJASC..5*5',AtCATGT…33.TGTAC^A...5PPflFI识别位点5；..GACN^N^TC「rS「3J.CTGlNM^NCAG…hPflMI识别位点5：.,CCANNHNtNTGG・・卫’3：..GGTN^N忡14忖舟GC..PhoI识别位点5…GG*CC…占3：CCJiG..5JPleI识别位点5J...GAGTC...3*PvuII识别位点5；,,dGTG…/扩…GTQpAC…5’PvuII识别位点5；,,dGTG…/扩…GTQpAC…5’RsaI识别位点,GT^C...3*-GAJC.-.S1RsrII识别位点亍…GCtWCGG…3「3JGCCWGfiC..5JSacI识别位点.GAGCT^..S'3：..CJCGAG..5^SacII识别位点5；,.CCGC'iSG,..y3：…GG^CGGC...5JSall识别位点护…&TCGAC…丁CAGCTtG.,.5JSapI识别位点『…OCTCTTC（呼.33,..CGr.5^Sau3AI识别位点.t&atq...3r3'..CTAG^.5*Sau96I识别位点..G'GNCC...3r3..CCNGp..,5JSbfI识别位点5：.CCTQCxTgG,,.3^3：..GG^CGTCD5^Seal识别位点5,,,AGT>QT,.3^对…TCAJGA…5’SfoI识别位点丁…gqpGG…5’SgrAI识别位点5；--CAtCCGGVG..3^扩…G¥G0CqpC…hSmaI识别位点5\..CCC^GC3S^.GGG^CCC5FSmlI识别位点VTYRAG...3-3；..GARYl^C...5rSnaBI识别位点5',,.TAC1&TA...3-3.ATGJ：AT...5^SpeI识别位点・/TCTAGT,,.3^3J...IGATC/.5JSerFI识别位点SerFI识别位点5\.C{7NGG..3r3..,GGNMCC..,5J作编号：GB8878185555334563BT9125XW作者：凤呜大王*SphI识别位点5\..GCATG't..S'r.C^TACG.SspI识别位点吕「・AAT^「T「・歹

3J...ITAJAA.5JStuI识别位点创JU--3J.TCC^GA.-.S*S