Z-YVAD-FMK-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEZ-YVAD-FMKCat.No.:HY-P1009CASNo.:210344-97-1分⼦式:C₃₁H₃₉FN₄O₉分⼦量:630.66Sequence:Z-Tyr-Val-Ala-Asp-FMKSequenceShortening:Z-YVAD-FMK作⽤靶点:Caspase作⽤通路:Apoptosis储存⽅式:Powder-80°C2years-20°C1yearInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:125mg/mL(198.21mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.5856mL7.9282mL15.8564mL5mM0.3171mL1.5856mL3.1713mL10mM0.1586mL0.7928mL1.5856mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(3.30mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(3.30mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(3.30mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Z-YVAD-FMK⼀种具有细胞通透性caspase-1和caspase-4抑制剂，具有抗炎和抗肿瘤活性。IC50&TargetCaspase体外研究Z-YVAD-FMK(100μM;24hours)significantlydownregulatedthegrowthinhibitioninducedbybutyrateinCaco-2cells[1].Z-YVAD-FMK(20μM;pre1hour;24hours)attenuatestheapoptoticinductionofIII-10onbothHepG2andBEL-7402cells,theapoptoticrateof-10onHepG2cellsisreducedbyZ-VAD-FMKfrom19.88%to8.34%,whilethatonBEL-7402cellsisreducedfrom17.56%to11.98%[4].CellViabilityAssay[1]CellLine:Caco-2cellsConcentration:0-100μMIncubationTime:24hoursResult:InhibitedCaco-2cellsgrowth.ApoptosisAnalysis[1]CellLine:BEL-7402andHepG2cellsConcentration:20μMIncubationTime:Pre1hour;24hoursResult:Inducedacaspase-dependentapoptosisincells.户使⽤本产品发表的科研⽂献•JHazardMater.2021Jan13;411:125134.•JAllergyClinImmunol.2022Mar26;S0091-6749(22)00382-7.•BiomedPharmacother.2022Jul;151:113098.•JVirol.2022May25;96(10):e0030922.•HumCell.2022Feb25.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].LiH1,etal.Aluminumhydroxideadjuvantsactivatecaspase-1andinduceIL-1betaandIL-18release.JImmunol.2007Apr15;178(8):5271-6.[2].Avivi-GreenC,etal.Differentmoleculareventsaccountforbutyrate-inducedapoptosisintwohumancoloncancercelllines.JNutr.2002Jul;132(7):1812-8.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplica