146种培养基及其配方1

)100g10gaCO320g(琼脂)15g1000mlAdjusttoNutrientAgar((蛋5gextract(牛肉膏)NaCl15gAdjust(pHto：Bacillus，5mgofMnSO4.H2Omaybeadded.istoformation、苏云金芽孢杆菌戈尔形)KH2PO40.2gK2HPO4MgSO4.7H2OCaSO4.2H2O0.1a2MoO4.2H2Oaxtract()Mannitol(甘)20gTract(微量)1000ml15gAdjust(调)CornMeal(玉玉5g(1gTap)1000mltheinat

1hr.the18

18,eachcontainsof,autoclave121hr.(15小18ⅹ18毫,每管61515MediumI10gKH2PO4Tomato)100mlTween(吐温)900mlMidiumLacto-casein10gextract(10g5g5gTween801gK2HPO4(醋5gcitrate2gMgSO4.7H2OMnSO4.H2O1000mextractPGY（（蛋白胨）10g（（1g1LGlycerol5gBeef3gGlycerol（20gTop（（15gpHeztract200mlMannitol（15g800mlpH

extract

Suspendfinelyandsoil200mlwater.121hr.Decantpaper,makeupvolume21,with(土5020015磅小200毫豇豆慢大豆根瘤菌、10.MannitolAgar()5g(3gMannitol)25g15g1000ml11.（10g0.5gK2HPO40.5gWater1000mlpH7.2-7.412.′sKNO31gstarch（20gK2HPO40.5gMgSO4.7H2ONaCl（20gpH7.2-7.4烬13.Wort（麦芽worldAdd15gintoword..Meltagtheinto110forminutes.稀释至)14.Potato)(1000ml葡)20g(20gSlicepotatoesAdd1000mlwater,topreventboilsoft.30min.).115℃for0minutes.（注：薯2001000毫301000毫升，克1515磅灭菌30分银白杨盘15.Czapek（察氏（30gNaNO33gMgSO4.7H2OKCl0.5gFeSO4.7H2OK2HPO4(13g蒸馏)1000mlpH16.(浓糖察200gNaNO3MgSO4.7H2OKCl0.5gFeSO4.4H2OK2HPO4(13g蒸馏)1000ml17.SyntheticPotato()20%Potato马)1L20gKH2PO4(硫胺素(微量)15gpH球毛壳葫串盘长孢（鲁银白杨盘长孢、瓶毛壳、金裙竹荪、灰树花、猴头菌、香菇、粗毛斗金（18.Meal(玉米粉meal[20:100(W/V)]well.mixture100ml,eachcontains30mlthemixture.at121(20%玉磅灭19.NH42SO4KH2PO4MgSO4.7H2ONaCl0.5g1000mlstrip620.Meal)10%infusionsoybean(10%)1000ml10gNaCl21.Wheat-branSawdustleaf75gbran25gPreparation

well.Addappropriateandamountmaybewithmixturemouth.Stickaholetheputcottonmouth克

25克20磅小22.Sowdust1021cmbiocksandimmersein.AlowtheorbingtheAutoclavefor1hr.(21磅1SawdustSawdust(松75%23-24%20for1hr.(20123.RiceMedium()dungcowUrea0.5%0.7%Gypsumpowder（Ammoniumsulphateappropriatewater,themupandthemfermentation.24.Medium（Rice

Selectmouldyandcut3inImmerseinhr.strawfullyamountaddedbywithiswhenofwaterbetween注]：3)25.Rice()10g50ml1212grice15

150mmtube).Addofat12130minutes.10克入50毫1515150630分)26.)KH2PO41gMgSO4.7H2OExtract((1000ml7cm(17

thetheliqidintotubecontainsoftheatminutes.Thenputortwoofoa(将上述溶液分配成溶液后分装入115分钟。1条227.ExtractAgarI酵母膏10gMall麦10g4g)1000ml(20gpH28.（Multi-peptone（2g1gGlucose（葡萄10g（20gDistilledwaterpH29.ExtractStarchAgar（10gextract10g（淀粉）NaCl5gaCO3（1L30.（Wheat36gNH42HPO4K2HPO40.2gMgSO4。0.1g（20gDistilledwaterpH31.Sporulation酵)KH2PO4K2HPO40.02%Sodiumacetate(0.5%0.062%NaCl(蛋白)0.25%2Agar()1ml/100ml

NaClCuSO4.5H2OFeSO4.4H2O0.2%0.1%(蛋NaCl0.1%KCl(0.25%Sodiumactate(0.82%(1.5%32.(BPY培(5g蛋白胨)NaClextract)5g(蒸1000ml33.)5g(蛋10gNaCl(1000ml34.(extract((胰蛋)5gNaCl1gK2HPO4.3H2O1000ml35.(蛋10g2gNaClNaH2PO4Sodium(5gBrain800mlinfusion(猪心浸200ml羊10%pHPreparationpigheartinfusionSmashup100gpig（withoutfatandvessel）.Add10200gpigAdddistilledto50℃1hr,boil15minutes;andthrough[注100克1000克1000350℃小154）冷却后用纱布36.Cellulose﹣decomposingSynthetic()NaCl0.1gKH2PO40.5gK2HPO42.0g(Distilledwater)1000mlFilterpaperstrip:strip(pH7.0-7.4So;idforCellulose2%2%2%羧甲）37.ClostridiumSynthetic（（MnSO4KH2PO4FeSO40.001%K2HPO4extract0.1%gSO4.7H2O0.02%0.01%0.001%CaCO30.5%Distille(蒸100%pH38.ExtractAgar（extract（4gAgar（17g1000mlpH39.OatemealOatemeal（]20g18%up（1000pH)：FeSO4.7H2OMnCl2.4H2OZnSO4.7H2ODistilledwater蒸)100ml40.(苹果)(苹)K2HPO40.5gGlycerol(甘)10g(15g蒸馏)1000ml41.()KH2PO4KClMgSO4.7H2O0.5ga(NO3)2.4H2OFeSO4.7H2O50g1000ml42.Medium(氧)KH2PO4CaCl2[]0.5gFeSO4.7H2O)1000mlpH43.(0.5%KH2PO4Na2HPO4.12H2OpH7.0-7.244.Milk(ATCC183100g45.(豌豆琼脂)蛋白0.5%NaCl2.0%infusion(counted[豌(以NH2mg∕100pH(before[7.2-7.5Preparetionpea豌Preparetion900gpea.thema5Lbottle,ofwater.pHtoAdd30mlchloroformforbindpthemouthgaugetoavoidtheoffgaspressure.the37Filtertheboilescapechloroform.Filautoclave120℃minutes..称豌豆900克5加自来3用至4.3-4.53020分℃30分Theofinfusionas()（:phosphoruse（auratus,transparent（46.)Wheat3.6%K2HPO4MgSO4.7H2O(pH47.ExtractAgar10g10g(水)1000ml(琼脂)pH48.MMNMedium(MMN)CaCl2MgSO4NaClKH2PO40.5g(100Be100mlCitric柠檬酸)900ml)49.StarchAgarstarch()NaNO31ggCO3K2HPO4(20g1000ml50.BeBe′51.Be′52.YEB(extract(蛋(MgSO4.7H2O(琼脂)pH53.(蛋10g()1g5gDdhro(1000mlAgar(15gpH54.YMMaltAgar)3.0g3.0g5.0g55.K2HPO4MgSO4.7H2ONaCl1.0gCaCO3FeSO4.7H2O蒸馏水1000ml56.Soy大豆胨2.5gNaClK2HPO457.3.0g5.0g蒸馏水1000mlpH6.6-7.058.(糖)1)5.0g3.0g(糖)琼59.22165.0g1.0g0.1gNaCl8.8gNa2SO4KClNaHCO3NH4NO30.0016gNa2HPO40.034g0.004g0.0024g蒸馏水1000mlpH7.4-7.860.ATCC(取)(Tryptose)NaCl6.6-7.061.5)1.0g1.0g2.0gFeSO4琼15.0g62.Trypticase(ATCCTrypticase63.(ATCC109)蛋白胨琼脂20.0g64.(ATCC1.0g1.0g1g5g2.5gMgSO4.7H2OK2HPO4自65.大豆蛋NaCl6.5g蒸馏水1000mlpH66.205.0gK2HPO43.0g葡2.0g蒸馏水1000mlpH7.3-7.567.酵母膏1.0gNaCl5.0g蒸馏水1000mlpH68.No.3,Difco)10.0g1.0gNaCl5.0g8.0g1.0gpH6.9-7.369.＇134)Czapek15.0g70.5.0g5.0g生物素K2HPO4NaCl0.1gMgSO4.7H2O酵2.5g蒸馏水1000ml(终)pH71.EclbBroth酵母膏NaCl氨(300pH72.1.0g1.0gNZ蒸馏水1000mlpH73.HarroldM40Y(ATCC酵母膏400.0g74.(Potato200g20g琼20gpH6.0-6.575.300g1.5g磺))pH76.牛肉膏3.0gpH7.0-7.277.4.0g4.0g78.(BrainHeart200.0g葡萄糖NaCl6.8-7.279.YeastPeptoneGlucose）蛋白胨琼脂20.0g80.ATCC340）20.0g煮1/281.ATCC423)200克去皮1000ml自来水1小时,加5.0mgMnSO4,pH用自来。加进15.0g琼82.ATCCMgSO4.7H2OZnSO4.7H2O5H2OFeSO4.7H2OCaCl2.2H2O0.1g称取,加入1000ml沸,多,83.ATCC527）K2HPO4MgSO4NaClNH42SO42.0gCaCO3FeSO4.7H2OZnSO4.7H2O20.0gpH7.0-7.484.2%孔(石)K4HPO41.5gMgSO4.7H2O85.琼脂12.5g将燕麦5-50oC，400ml，86.ATCC573）NH42SO41.3gK2HPO4MgSO4.7H2O1.0g1.0gH2SO4，琼脂50oC，87.ATCC593）500.0gNaOH25.0ml所用瘦NaOH1000ml。滤酵母膏K2HPO45.0gResazurin0.5gL-H2O。O2-freenitrogen）3%氢份88.YMPG3.0g3.0g5.0gFeSO4.7H2O20.0g110oC，35分89.TGYM培(5.0g1.0g3.0gDL-DL-Meth0.5g90.(3.0g3.0g3.0gK2HPO41.0g蒸馏水1000mlpH91.Na2HPO4KH2PO43.0gNaClNH4Cl(单)维生素1pH自92.微量元1mlpHFeSO4.7H2OMnCl2.4H2OZnSO4.7H2O93.OatmealAgar5.0g500ml40.0g250ml1000mlpH5.894.(AYCC200g蒸馏水1000mlpH95.Czapek-Dox(ATCC312)NaNO33.0gK2HPO4MgSO4.7H2OKCl0.5gFeSO4.7H2O单)蒸馏水1000mlpH96.GC培养基(ATCCA5.0g琼(BBL500.0mlB液(500.0ml(BBL97.K2HPO40.1gKH2PO4MgSO4.7H2ONaCl0.1gCaCl2NaMoO4.2H2O(Na蒸馏水1000mlpH7.2-7.498.NaNO31.5gK2HPO4MgSO4.7H2OCaCl2.7H2ONa2CO30.006g0.006g*微1ml)50微量元A5H3BO42.86gZnSO4Na2MoO4CuSO4.5H2OCo(NO3)2.6H2O99.5.0g1.0g0.1gNaClgSO4.7H2Oa2SO4CaCl2NaHCO310mlpH7.0-7.6微量元0.034gH3BO4NaClNaNO30.0016gNaHPO40.008g1000ml2.0g10.0g蒸馏水1000mlpH7.2-7.4101.GYMC4.0g2.0g琼脂20.0gCaCO31.5g蒸pH6.8-7.0LCSB玉米浆5.0gNaCl4.0gMgSO4.7H2OKH2PO40.6gFeCl3.6H2OCuSO4.5H2O蒸馏水1000ml4.81KNO3K2HPO40.5gMgSO4.7H2ONaClFeSO4.7H2O蒸馏水1000mlpH7.0-7.2-酵(5.0g1.0gKH2SO41.0g1.0g蒸馏水pH(Acid)ATCCNa2HPO4.12H2OKH2PO4MgSO4.7H2O柠CaCl220.0mg1.0mgTrypticcase(BBL4.0g20.0pH牛肉膏3.0g5.0gNaCl1.0g0.5g3.0g蒸馏水1000mlpH6.6-7.0-甘()醇K2HPO40.5gMgSO4.7H2ONaCl(糖)0.4g1000mlDextrinAgar(ATCC糊精20.0gCoCl2.6H2O蒸馏水1000mlpH7.2-7.4FeSO41.0mgMgSO4.7H2O200.0mgNa2HPO4NaH2PO4KCl40.0mg15.0mgNaNO3见0.1%(v/v)CuSO4.5H2OH3BO4MnSO4.5H2O7.0mcgZnSO4.7H2O7.0mgMoO3CoSO4.7H2O18.0mcg110.(AS1)100.0gCaCO3(AS天冬酰(Asparagine)0.5gK2HPO40.5g蒸pH7.2-7.4112.(AS13)12Brix(-)1.5%110℃30分113.ATCC805）1.0gK2HPO40.7gMgSO4.7H2O甘5.0gKH2PO40.1g蒸7.4500.0g2.0gNaCl2.0gNa2HPO40.4gNa2CO3蒸馏水1000mlpH1.0g蒸馏水1000mlpH116.（AS14500g称取500即加1000ml棉布液用水1000ml20分117.PCA（343）胡萝卜蒸馏水1000mlpH118.Mueller-Hinton300.0g1.5g50蒸馏水1000mlpH119.AS20葡萄糖NaCl（AS4813.0gNaCl28.0gK3PO4.3H2O0.4g）Fe+,Mn+2营(AS100)蛋白胨NaCl15.0-20.0gpH122.(Trypticase大豆胨2.5gNaCl5.0gK2HPO42.5g甘蒸馏水1000mlpH(DSMK2HPO40.8gKH2PO4MgSO4.7H2OCaCl2.2H2OFeSO4.7H2OMnSO4.H2O（315.0g3.0g蒸馏水1000ml1MNa-溶液：。NaNaHCO3Na2CO35.3g100.0ml125.TrypticaseSoy220）fromcasein）豆粉蛋soymeal）5.0gNaCl5.0IFO203酵母膏葡萄糖GlycerolNaCl蒸馏水1000mlpH，IFO2311.0g1.0gNZ胺AminetypeA麦Maltose）蒸馏水1000mlpHSheffieldRamonCA94583USAor，（YE大豆蛋NaCl