合作课题与NIH申请书的书写

合作课题与NIH申请书的书写合作课题申请：课题分担：课题承担量大，需多实验室合作；研究资源互补：人力资源、现场资源或动物资源优势与技术资源互补；研究能力互补：细胞学、分子生物学、新型技术与实验方法Significance:TheCIPRAcohortoffersauniquepopulationinfectedataboutthesametimewithauniformHIVstrain.Determiningimmunologicparametersthatdifferentiateprogressionofdiseasefromnon-progressioncanbequitesignificant.Approach:ThisapplicationfromagroupofinvestigatorsinChinaaimstoevaluatetwocohortsofHIVinfectedpatientsfromtheCIPRAprojecttodeterminefunctionalityofCD8epitope-specificimmuneresponsesduringHIV-1infectionandcorrelatethesewithprogressionorcontainmentofHIVreplication.ThiscohortcontainspatientswhowereinfectedwithHIVabout10yearsagothroughcontaminatedbloodproducts.AllwereinfectedbytheThailandBvirus.Innovation:ThestudyofCD8responsestospecificviralepitopesandcorrelationwithTregfunctioninprogressorvs.non-progressorpopulationsisinnovative.GrantApplication:FivecomponentsfromeachreviewerInvestigators:ThePI,Dr.Xu,isexperiencedinHIVimmunologyresearch.Hisresearchteamhasrelevantexperienceinbiostatisticsandmolecularvirology;however,theresponsibilitiesofeachteammemberarenotdescribed.Environment:ThePI’slaboratoryiswellequippedtocarryouttheproposedwork.ClinicalresourcescriticaltotheprojectaredemonstratedinthepreliminaryData,butapparentlyarequitedistantfromthelaboratory,requiringdeliveryofspecimensbyaircraft,whichmaybeproblematic.FivecomponentsfromeachreviewerInnovation:ThestudyofCD8responsestospecificviralepitopesandcorrelationwithTregfunctioninprogressorvs.non-progressorpopulationsisinnovative.Innovation:Theproposedprojectwilladdressacriticalbarriertoprogressinthefield.Aftermorethan20yearswestilldonotcompletelyunderstandtheimmunopathogenesisofHIVinfection.Itisthoughtthatinformationgainedbycomparingimmune,viral,andgeneticdifferencesbetweenprogressorsandlong-termnon-progressorswillbeusefulindefiningthehost’sresponsetoHIVinfectionwhichiscrucialforthedevelopmentofanHIVvaccine.TheproposedstudieswillexpandthebreadthofresearchsupportandadvancethedevelopmentoflocalscientificexpertiseinBeijingaswellasprovidesupportfornewChineseinvestigators.Innovation:Thelevelofinnovationislow;theprojectproposestouseestablishedtechniques,althoughthesearestate-of-the-artinHIVimmunologyandappropriatefortheresearchobjectives.DifferentcommentsfromdifferentreviewersfortheidenticalapplicationInvestigators:ThePI,Dr.Xu,isexperiencedinHIVimmunologyresearch.Hisresearchteamhasrelevantexperienceinbiostatisticsandmolecularvirology;however,theresponsibilitiesofeachteammemberarenotdescribed.Investigators:Theinvestigatorsareappropriatelytrainedandwellsuitedtoconductthisresearch.Dr.JianqingXuwastrainedattheResearchInstituteforGeneticandHumanTherapyinWashington,D.C.,from1997-2004andhasanumberofpublicationsfromthistraining.ItappearsthatDr.XuhasbeenabletotransfermuchofthistechnologytotheChineseCDCinBeijing.Environment:ThePI’slaboratoryiswellequippedtocarryouttheproposedwork.ClinicalresourcescriticaltotheprojectaredemonstratedinthepreliminaryData,butapparentlyarequitedistantfromthelaboratory,requiringdeliveryofspecimensbyaircraft,whichmaybeproblematic.Environment:ThePIandcollaboratingteamhasastrongtrackrecordandtheuseoftheCIPRAcohortforseveralyearsmakesforanexcellentenvironmentforthesestudies.Facilitiesareadequate.DifferentcommentsfromdifferentreviewersfortheidenticalapplicationTHEFOLLOWINGRESUMESECTIONSWEREPREPAREDBYTHESCIENTIFICREVIEWADMINISTRATORTOSUMMARIZETHEOUTCOMEOFDISCUSSIONSOFTHEREVIEWCOMMITTEEONTHEFOLLOWINGISSUES:PROTECTIONOFHUMANSUBJECTS(Resume):ACCEPTABLE(30).Informedconsentseemsadequate;privacyprotectionisinplace;optionsfortreatmentarewelldefined.However,thestudyproceduresandpotentialrisksarenotdescribed.Thetextshouldalsoaddressplansto“collectadditionalbloodifnecessary”.INCLUSIONOFWOMENPLAN(Resume):ACCEPTABLE(G1A).However,thespecificsrelatedtorecruitmentofwomenarelacking.INCLUSIONOFMINORITIESPLAN(Resume):ACCEPTABLE(M5A).However,thespecificsrelatedtorecruitmentofminoritiesarelacking.INCLUSIONOFCHILDRENPLAN(Resume):ACCEPTABLE(C1A).BIOHAZARDCOMMENT:Protectionagainstblood-bornepathogensshouldbeaddressed.FOREIGNINSTITUTION:Theproposedprojectutilizesauniquesubjectcohortattheforeignlocation,andsupportstheactivitiesoflocalresearchersCOMMITTEEBUDGETRECOMMENDATIONS:Thebudgetwasrecommendedasrequested.基本原则：核心思维：目的明确，假说有力面面俱到：含所有的必需元件路标意识：研究思路清晰具体，具有可操作性初次相见：简略介绍相关背景Significance:Thesignificanceofthisresearchmustbegiventhehighestpossiblerating.

Approach:Takentogether,theseproposedstudiesare–toputitmildly–ratherambitious.Theproposallackscriticaldetail,focusandinterpretiveprecisionneededtopreciselydefinewhatcomparisonsaretobemade.

Innovation:Mostofthedataaredescriptive,however,theyhavethepotentialtoleadtohighlyrelevantandimportantissuesinHIVimmunopathogenesis.Inves