TBHQ-tert-Butylhydroquinone-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETBHQCat.No.:HY-100489CASNo.:1948-33-0Synonyms:tert-Butylhydroquinone分⼦式:C₁₀H₁₄O₂分⼦量:166.22作⽤靶点:Keap1-Nrf2;ERK;Autophagy;Apoptosis;Ferroptosis作⽤通路:NF-κB;MAPK/ERKPathway;StemCell/Wnt;Autophagy;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥56.66mg/mL(340.87mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM6.0161mL30.0806mL60.1612mL5mM1.2032mL6.0161mL12.0322mL10mM0.6016mL3.0081mL6.0161mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀1/4MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(15.04mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(15.04mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(15.04mM);Clearsolution4.请依序添加每种溶剂：50%PEG300>>50%salineSolubility:20mg/mL(120.32mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性TBHQ(tert-Butylhydroquinone)⼀种⼴泛使⽤的Nrf2激活剂，通过激活Nrf2来免受Doxorubicin(DOX)-诱导的⼼脏毒性[1]。TBHQ(tert-Butylhydroquinone)也⼀种ERK激活剂，逆转Dehydrocorydaline(DHC)对⿊素瘤细胞增殖的抑制作⽤[2]。IC50&TargetERKNrf2Autophagy体外研究TBHQ(t-butylhydroquinone;tBHQ;0-100μM;48hours;H9c2cells)alonedoesnotaffectH9c2cellsviability.Pre-incubationoftheH9c2cellswithvariousconcentrationsoftBHQfor24hoursenhancescellviabilitywhichisdecreasedduetoexposuretoethanolinadose-dependentmanner.TreatmentwithtBHQmarkedlyenhancestheviabilityofH9c2cardiomyocytesexposedtoethanol[3].TBHQ(5μM;15min;H9c2cells)treatmentsignificantlyreducestheamountofapoptoticcellsexposedtoethanol[3].TBHQ(5μM;H9c2cells)pre-treatmentmarkedlyinhibitestheethanol-inducedincreaseincaspase-3andBaxexpression,andenhancesBcl-2expression[3].CellViabilityAssay[3]CellLine:H9c2cellsConcentration:0µM,0.625µM,1.25µM,2.5µM,5µM,10µM,20µM,50µMand100µMIncubationTime:48hoursResult:EnhancedtheviabilityofH9c2cardiomyocytesexposedtoethanol.ApoptosisAnalysis[3]CellLine:H9c2cellsConcentration:5μMIncubationTime:Result:Loweredtheamountofapoptoticcellsexposedtoethanol.2/4MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEWesternBlotAnalysis[3]CellLine:H9c2cellsConcentration:5μMIncubationTime:Result:Inhibitedtheethanol-inducedincreaseincaspase-3andBaxexpression,andenhancedBcl-2expression.体内研究TBHQtreatment(50mg/kg;Intraperitonealinjection;threeinjectionsatintervalsof8hthatbegan1-hpostICH;CD-1mice)augmentstheDNA-BindingactivityofNrf2,attenuatesoxidativebraindamageandacuteneurologicaldeficitsafterintracerebralhemorrhage(ICH),attenuatesmicroglialactivationwithconcomitantreductioninthereleaseofproinflammatorycytokineinterleukin-1β(IL-1β).TBHQhastheefficacyofpost-injuryadministrationinattenuatingacuteneurologicalinjuryafterICH[4].AnimalModel:MaleCD-1mice(8-10weeksold)[4]Dosage:50mg/kgAdministration:Intraperitonealinjection;threeinjectionsatintervalsof8hoursthatbegan1hpostICH.Result:ThetreatmentaugmentedtheDNA-bindingactivityofNrf2,attenuatedbrainoxidativedamage,attenuatedthemicroglialactivationandtheexpressionofIL-1β.户使⽤本产品发表的科研⽂献•ProcNatlAcadSciUSA.2019Feb19;116(8):2996-3005.•Theranostics.2021Jan1;11(2):861-877.•RedoxBiol.2022Jul31;55:102408.•IntJBiolSci.2022;18(16):6189-6209.•CellDeathDis.2022Aug25;13(8):733.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].Lin-FengWang,etal.Tert-butylhydroquinoneamelioratesdoxorubicin-inducedcardiotoxicitybyactivatingNrf2andinducingtheexpressionofitstargetgenes.AmJTranslRes.2015;7(10):1724–1735.[2].HuH,etal.Dehydrocorydalineinhibitscellproliferation,migrationandinvasionviasuppressingMEK1/2-ERK1/2cascadeinmelanoma.OncoTargetsTher.2019Jul2;12:5163-5175.[3].XIAOJINGSHI,etal.Tert-butylhydroquinoneattenuatestheethanol-inducedapoptosisofandactivatestheNrf2antioxidantdefensepathwayinH9c2cardiomyocytes.IntJMolMed.2016Jul;38(1):123–130.3/4MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[4].Sukumari-RameshS,etal.Post-InjuryAdministrationofTert-butylhydroquinoneAttenuatesAcuteNeurologicalInjuryAfterIntracerebralHemorrhageinMice.JMo