PSB-12379-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPSB-12379Cat.No.:HY-100747CASNo.:1802226-78-3分⼦式:C₁₉H₂₅N₅O₉P₂分⼦量:529.38作⽤靶点:CD73作⽤通路:Immunology/Inflammation储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验H2O:125mg/mL(236.13mM;Needultrasonic)DMSO:≥5mg/mL(9.45mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM1.8890mL9.4450mL18.8900mL5mM0.3778mL1.8890mL3.7780mL10mM0.1889mL0.9445mL1.8890mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：PBSSolubility:100mg/mL(188.90mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性PSB-12379⼀种核苷酸类似物，⼀种有效的Ecto-5'-Nucleotidase(CD73)抑制剂，Kis值为9.03nM(⿏)和2.21nM(⼈)。IC50&TargetKi:9.03nM(rat),2.21nM(human)(Ecto-5’-Nucleotidase)[1]体外研究Metabolism:Onlyasmallpercentage(77%ofthecompoundsarerecoveredunchangedafteralongincubationfor8h.PSB-12379(10g)issomewhatlessstable,and56%aremetabolizedwithin8h.Hydrolyticcleavageoftheglycosidicbond,whichisatypicalphaseIreactionofnucleosidesandnucleotides,wasobservedasthemainreaction[1].户使⽤本产品发表的科研⽂献•FrontBioengBiotechnol.2022Apr27;10:895998.•Cancers(Basel).2022,14(23),5750Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].BhattaraiS,etal.α,β-Methylene-ADP(AOPCP)DerivativesandAnalogues:DevelopmentofPotentandSelectiveecto-5'-Nucleotidase(CD73)Inhibitors.JMedChem.2015Aug13;58(15):6248-63.[2].X-RayCo-CrystalStructureGuidestheWaytoSubnanomolarCompetitiveEcto-5-Nucleotidase(CD73)InhibitorsforCancerImmunotherapySanjayBhattarai.Adv.Therap.2019,2,1900075.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.T