ML348-GNF-Pf-1127-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEML348Cat.No.:HY-100736CASNo.:899713-86-1Synonyms:GNF-Pf-1127分⼦式:C₁₈H₁₇ClF₃N₃O₃分⼦量:415.79作⽤靶点:Phospholipase作⽤通路:MetabolicEnzyme/Protease储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:25mg/mL(60.13mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.4051mL12.0253mL24.0506mL5mM0.4810mL2.4051mL4.8101mL10mM0.2405mL1.2025mL2.4051mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>90%cornoil1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(6.01mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性ML348(GNF-Pf-1127)⼀个选择性的和可逆的酰蛋⽩硫酯酶1(APT1)/溶⾎磷脂酶1(LYPLA1)抑制剂,IC50值为210nM[1]。IC50&TargetIC50:210nM(LYPLA1)体外研究ML348(5µM;3hours)selectivelyinhibitsLYPLA1inculturedcells[1].CellViabilityAssay[1]CellLine:HEK293Tcells,murineT-cellsConcentration:5µMIncubationTime:3hoursResult:Showpotent,selectiveinhibitionofLYPLA1.体内研究ML348(50mg/kg,i.p.,3hours)showspotentandselectiveactivityinvivo,inhibitsLYPLA1enzymesinheart,kidneyandlunginmice[1].AnimalModel:C57BL6malemice(3-4months)[1]Dosage:50mg/kgAdministration:Intraperitonealinjection;3hoursResult:PotentandselectiveinhibitedLYPLA1enzymesinheart,kidneyandlunginvivo.户使⽤本产品发表的科研⽂献•AdvMater.2021Dec;33(49):e2103471.•NatBiomedEng.2019Apr;3(4):306-317.•NatCommun.2020Sep21;11(1):4765.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].AdibekianAetal.CharacterizationofaSelective,ReversibleInhibitorofLysophospholipase1(LYPLA1).NationalCenterforBiotechnologyInformation(US);2010-2013Apr08.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly