LRE1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELRE1Cat.No.:HY-100524CASNo.:1252362-53-0分⼦式:C₁₂H₁₃ClN₄S分⼦量:280.78作⽤靶点:AdenylateCyclase作⽤通路:GPCR/GProtein储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:125mg/mL(445.19mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.5615mL17.8075mL35.6151mL5mM0.7123mL3.5615mL7.1230mL10mM0.3562mL1.7808mL3.5615mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(7.41mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(7.41mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性LRE1可溶性腺苷酸环化酶的特异性和变构抑制剂。IC50&TargetAdenylylcyclase[1]体外研究LRE1bindstothebicarbonateactivatorbindingsiteandinhibitssolubleadenylylcyclase(sAC)viaauniqueallostericmechanism[1].LRE1preventssAC-dependentprocessesincellularandphysiologicalsystemsandfacilitatesexplorationofthetherapeuticpotentialofsACinhibition[1].LRE1(0.5-100μM,60minutes)inhibitsspermandmitochondrialfunctionsofsAC[1].WesternBlotAnalysis[1]CellLine:MousecaudaspermConcentration:0.5-100µMIncubationTime:60minutesResult:LRE1inhibitssACdependentspermcapacitation[1].户使⽤本产品发表的科研⽂献•FrontCellDevBiol.2021Nov17;9:772254.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].Ramos-EspirituL,etal.DiscoveryofLRE1asaspecificandallostericinhibitorofsolubleadenylylcyclase.NatChemBiol.2016Oct;12(10):838-44.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400