F1063-0967-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEF1063-0967Cat.No.:HY-101510CASNo.:613225-56-2分⼦式:C₂₄H₂₄N₂O₅S₂分⼦量:484.59作⽤靶点:Phosphatase作⽤通路:MetabolicEnzyme/Protease储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:100mg/mL(206.36mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.0636mL10.3180mL20.6360mL5mM0.4127mL2.0636mL4.1272mL10mM0.2064mL1.0318mL2.0636mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(5.16mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性F1063-0967⼀种双特异性磷酸酶26(DUSP26)抑制剂，其IC50值为11.62μM。IC50&TargetIC50:11.62μM(DUSP26)[1]体外研究F1063-0967isaDual-specificityphosphatase26(DUSP26)inhibitorwithanIC50of11.62μM.Atconcentrationabove0.1μM,F1063-0967remarkablyinducesIMR32cellapoptosis.F1063-0967inducesapoptosisinIMR-32celllinewithanIC50valueof4.13μM.F1063-0967hasnoeffectonHL7702cellline,andlittleinhibitioneffectonSH-SY5Ycellline[1].PROTOCOLCellAssay[1]TheIMR-32cellsareplatedin96-wellplateswith1×103cellsperwell.Afterthecellsareallowedtosettlefor24h,F1063-0967(0,10,1,and0.1μM)isadded.AnMTTassayisperformed.ForcelllinesHL7702andSH-SY5Y,thecellsareallowedtosettlefor24h,F1063-0967(10μM)isadded,thenMTTassaysarecarriedoutafter72h[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.REFERENCES[1].RenJX,etal.Identificationofnoveldual-specificityphosphatase26inhibitorsbyahybridvirtualscreeningapproachbasedonpharmacophoreandmoleculardocking.BiomedPharmacother.2017May;89:376-385.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseon