Cjoc42-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECjoc42Cat.No.:HY-138054CASNo.:2171519-89-2分⼦式:C₂₀H₂₁N₃O₅S分⼦量:415.46作⽤靶点:MDM-2/p53作⽤通路:Apoptosis储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:100mg/mL(240.70mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.4070mL12.0349mL24.0697mL5mM0.4814mL2.4070mL4.8139mL10mM0.2407mL1.2035mL2.4070mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(6.02mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性Cjoc42⼀种能够与gankyrin结合的化合物。Cjoc42以剂量依赖性⽅式抑制gankyrin活性。Cjoc42可防⽌通常与⼤量gankyrin相关的p53蛋⽩⽔平降低。Cjoc42恢复p53依赖性转录和对DNA损伤的敏感性[1]。体外研究cjoc42(0.5,1,and5μM;48hours)dose-dependentlyrestoresp53levels(comparabletomocktransfectedcells)andinhibitsgankyrin-inducedloweringofp53levelsincells[1].cjoc42(0.1,1,and10μM;24hours)aloneshowsnodose-dependentchangeincellviability[1].WesternBlotAnalysis[1]CellLine:U2OSConcentration:0.5,1,and5μMIncubationTime:48hoursResult:Dose-dependentrestorationofp53levels(comparabletomocktransfectedcells)andinhibitedgankyrin-inducedloweringofp53levelsincells.REFERENCES[1].ChattopadhyayA,etal.Discoveryofasmall-moleculebinderoftheoncoproteingankyrinthatmodulatesgankyrinactivityinthecell.SciRep.2016;6:23732.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3