AMPC-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEAMPCCat.No.:HY-139065CASNo.:2254434-33-6分⼦式:C₂₅H₁₆FN₃O₃分⼦量:425.41作⽤靶点:Others作⽤通路:Others储存⽅式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验DMSO:115mg/mL(270.33mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.3507mL11.7534mL23.5067mL5mM0.4701mL2.3507mL4.7013mL10mM0.2351mL1.1753mL2.3507mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥6mg/mL(14.10mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(5.88mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性AMPC⼀种有效的TFF3抑制剂。在TFF3阳性的CMS4结直肠癌细胞中，AMPC可抑制细胞增殖、存活、致瘤性和CSC样⾏为。AMPC作为⼀种潜在的抗癌试剂，可以单独或和5-FU联合使⽤，⽤于癌症研究。体外研究AMPCdecreasescellproliferationinCMS4CRCcells,theIC50valuesare2.63μM,4.65μM,and69.69μM,respectivelyinSW620(highTFF3expression),Caco-2,SW480cells(lowTFF3expression),respectively[1].AMPC(1-10μM)significantlyreducesthetotalcellularlevelsofTFF3inSW620andCaco2cellsinadosedependentmanner[1].AMPCinducesapoptosisinadosedependentmannerbytheinhibitionofTFF3,itinducesadecreaseintheS-phasepopulationandincreasescaspase3/7activitycomparedwithcontrolcells[1].体内研究AMPC(intraperitonealinjection;40mg/kg;oncedaily)leadstoasignificantreductionoftumourvolumeinAMPC-treatedmiceascomparedtovehicle-treatedmicefromday11onward.AMPCresultsinlargerareasoftumournecrosisandincreasedareaofcellswithapoptoticfeaturesinSW620tumours.AMPCalsosignificantlyreducestumourandserumTFF3levelsinvivo.AnimalModel:SW620cellsaresubcutaneouslyinjectedintonudemice[1]Dosage:40mg/kgAdministration:Intraperitonealinjection;40mg/kg;oncedailyResult:Leadtotumordecreaseinvivo.ResultsinamarkedreductionofKi67expressionintumor.REFERENCES[1].Ru-MeiChen,etal.PharmacologicalInhibitionofTFF3EnhancesSensitivityofCMS4ColorectalCarcinomato5-FluorouracilthroughInhibitionofp44/42MAPK.IntJMolSci.2019Dec9;20(24):6215McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplicati