Ionomycin-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrarieswww.MedChemEIonomycinCat.No.:HY-13434CASNo.:56092-81-0分⼦式:C₄₁H₇₂O₉分⼦量:709.01作⽤靶点:CalciumChannel;PKC;Bacterial;Apoptosis;Antibiotic作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling;Epigenetics;TGF-beta/Smad;Anti-infection;ApoptosisSolution,-20°C,2years储存⽅式:溶解性数据体外实验Ethanol:≥10mg/mL(14.10mM)扫描⼆维码，*"≥"meanssoluble,butsaturationunknown.运⽤溶解⽅案计算器获得适合您实验体系的溶解⽅案MassSolvent1mg5mg10mgConcentration制备储备液1mM1.4104mL7.0521mL14.1042mL5mM0.2821mL1.4104mL2.8208mL10mM0.1410mL0.7052mL1.4104mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。BIOLOGICALACTIVITY⽣物活性Ionomycin(SQ23377)⼀种有效的，选择性的钙离⼦载体(calciumionophore)，也⼀种由Streptomycesconglobatus产⽣的抗⽣素。Ionomycin(SQ23377)对⼆价阳离⼦具有⾼度特异性(Ca>Mg>Sr=Ba)。Ionomycin(SQ23377)促进细胞凋亡。Ionomycin(SQ23377)还诱导蛋⽩激酶C(PKC)激活。IC50&TargetCalciumionophore[1]体外研究IonomycinisaCalciumionophoreandanantibioticproducedbyStreptomycesconglobatus[1].1/3www.MedChemEwww.MedChemEAdditionof2μMIonomycintoLCLC103HcellscausesaninstantaneousincreaseinintracellularCa2+concentrationfrom50to180nM.DNAandproteinanalysisinIonomycin-treatedculturesrevealedDNAfragmentationandPARPcleavagetoan85-kDafragmenttypicalofcaspase-mediatedapoptosis.Necrosiscouldbedetectedin~1-5%oftheIonomycintreatedcells.CaspaseactivationinwholecellswasfollowedbymonitoringtheincreaseinactivityagainstAc-DEVD-amcfollowingIonomycintreatment[2].Ionomycin-mediatedcleavageandexosomerelease.FollowingIonomycinexposure,mediumconditionedbySKOV3ipcellshadincreasedamountsofexosomescontainingtheL1-32cleavagefragment[4].户使⽤本产品发表的科研⽂献•SciTranslMed.2020Nov25;12(571):eaaz6667.•SciTotalEnviron.2021,146523.•CellDeathDis.2021Apr1;12(4):336.•JImmunol.2021Mar1;206(5):963-977.•Aging.2019Aug19;11(16):6490-6502.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LiuC,etal.Characterizationofionomycinasacalciumionophore.JBiolChem.1978Sep10;253(17):5892-4.[2].ChatilaT,etal.MechanismsofTcellactivationbythecalciumionophoreionomycin.JImmunol.1989Aug15;143(4):1283-9.[3].Gil-ParradoS,etal.Ionomycin-activatedcalpaintriggersapoptosis.AprobableroleforBcl-2familymembers.JBiolChem.2002Jul26;277(30):27217-26.[4].StoeckA,etalAroleforexosomesintheconstitutiveandstimulus-inducedectodomaincleavageofL1andCD44.BiochemJ.2006Feb1;393(Pt3):609-18.McePdfHeight2/3www.MedChemEwww.MedChemE关注MCE中国公众号，

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