细菌抗菌肽的分类、作用机理及基因工程研究进展

细菌抗菌肽的分类、作用机理及基因工程研究进展刘小雨2023年10月目录1抗菌肽的分类21.1来源于细菌的抗菌肽2来源于芽孢杆菌只抗革兰氏阴性菌（G-）的抗菌肽7来源于芽孢杆菌抗真菌的的抗菌肽7来源于其它物种抗真菌同时抗的革兰氏阴性菌（G-）的抗菌肽102抗菌肽的作用机理112.1细胞膜电势依赖通道的形成112.2抑制细胞呼吸作用112.3抑制细胞外膜蛋白的合成112.4抑制细胞壁的形成112.5进入细胞核结合到DNA或RNA上影响细胞113抗菌肽在各个表达系统中的表达特点及研究进展123.1抗菌肽在大肠杆菌表达系统中的研究进展12密码子偏爱性改造123.1.2串联表达123.1.3融合表达133.1.4杂合表达133.2抗菌肽在酵母表达系统中的研究进展134国内基因工程改造的相关专利技术144.1国内专利技术144.2外源蛋白在枯草芽孢杆菌中表达可供选择的技术路线154.2.1复制质粒融合表达路线164.2.2代谢途径调控路线164.2.3基因定点突变路线165抗菌肽及其工业应用前景16目前，由于抗生素在临床治疗中的不规范使用，不仅造成经济上的巨大损失和药物不良反应；而且导致细菌和真菌等病原微生物正在以惊人的速度对抗生素产生强耐药性。特别是多重耐药菌和超级耐药菌的出现，经常在很短时间内出现全世界范围内的流行，给人们的生命安全和经济带来巨大损失。因此对具有高抗菌活性和安全性抗菌肽的研发日益受到重视，人们期望抗菌肽成为抗生素的理想替代品之一。抗菌肽（AMPS）一般由10~60个氨基酸残基组成，分子量约为2000~7000Da，并且热稳定性较好。抗菌肽的抗菌机制不同于传统抗生素，其作用靶点大致分为细胞膜作用靶点和胞内作用靶点(DNA，RNA，酶等)两大类，抗菌肽的抗菌活性和毒性与抗菌肽的二级结构、净电荷数量和疏水性等理化参数密切相关。迄今为止，大约有2700多种抗菌肽已经被分离出来，部分已经通过化学合成和基因工程表达大规模制备。在实际应用方面，部分抗菌肽已成功应用于医疗卫生和食品加工等行业，其中尤其引人注意的是多种抗菌肽已经进入二、三期临床试验阶段【【】Neetoo,H.,M.Ye,andH.Q.Chen,【】Neetoo,H.,M.Ye,andH.Q.Chen,PotentialantimicrobialstocontrolListeriamonocytogenesinvacuum-packagedcold-smokedsalmonpateandfillets.InternationalJournalofFoodMicrobiology,2023.123(3):p.220-227.【】Lazarev,V.N.andV.M.Govorun,Antimicrobialpeptidesandtheiruseinmedicine.AppliedBiochemistryandMicrobiology,2023.46(9):p.803-814抗菌肽的来源与制备大致分为三条途径：天然产物(微生物，植物，动物，海洋生物来源)提取，化学合成（液相合成法和固相合成法）和基因工程（大肠杆菌，枯草芽孢杆菌和毕赤酵母表达载体等）制备。天然产物提取易受来源限制，特别是来自珍稀动植物资源的抗菌肽，提取更易受到原材料来源限制。化学合成的生产成本较高，且有多种剧毒性试剂（氢氟酸、氰化钾等）需要大量应用，产生的“三废”对环境造成的污染问题也难以解决。通过基因工程和发酵工程的方法制备抗菌肽可以有效降低生产成本，生产工艺容易放大；产生的废液处理工艺较为成熟。目前使用原核表达系统制备抗菌肽占所有抗菌肽基因表达系统的80%左右。人们使用原核表达系统表达抗菌肽已经积累了许多经验，对抗菌肽表达质粒构建，生产工艺的中试和放大也有所进展。但是抗菌肽的原核表达依然具有很大技术挑战性，其不仅对宿主菌有毒性，而且由于抗菌肽分子量较少，易被宿主菌各种内源性蛋白酶降解。以往研究表明抗菌肽采用与融合标签表达是实现抗菌肽高效表达的有效策略之一。目前常用的融合标签有硫氧还蛋白（Trx）、类固醇异构酶（KSI）及谷胱甘肽-S-转移酶（GST）等，但在实际应用中，发现上述标签存在标签蛋白过大、促可溶性效果差、表达产物剪切效率低等问题，因此寻找提高抗菌肽的可溶性表达和产率方法依然是目前研究的重要方向。1抗菌肽的分类按照抗菌肽的来源分类，截止到2023年7月，共有2717种抗菌肽被公布，其中来源于细菌的有269种，来源于古生菌的有4种，来源于原生生物的有8种，来源于真菌的有13种，来源于植物的有333种，来源于动物的有2042种。由于在芽孢杆菌表达系统中，缺少真核以及动物、植物等相应的修饰系统，来源于动物、植物以及真菌的基因在原核表达系统中很难得到高效表达，所以本综述中重点关注来自于细菌的抗菌肽及其抗菌活性；其具体信息如下：1.1来源于细菌的抗菌肽来源于细菌的抗菌肽一共有269种，其部分信息如下：NameSourceActivityNameSourceActivityHominicinStaphylococcushominis

MBBL2-9anti-Gram+anti-Gram-TricyclicpeptideRP71955Streptomycesstrains

AA3891Antiviralanti-HIVPlantaricinALactobacillusplantarum

C11anti-Gram+anti-Gram-CancercellsCarnobacteriocinB2Carnobacteriumpiscicolaanti-Gram+anti-Gram-LeucocinALeuconostocgelidum

UAL-187anti-Gram+anti-Gram-NisinZLactococcuslactisSIK-83anti-Gram+CancercellsNisinAStreptococcuslactisanti-Gram+SpermicidalAntibiofilmWoundhealing,CancercellsSubtilinBacillussubtilisanti-Gram+Pep5Staphylococcusepidermidis

strain5anti-Gram+MicrocinJ25Escherichiacoli

AY25anti-Gram-GramicidinAsoilbacterium,

Bacillusbrevis（短杆菌）anti-Gram+anti-Gram-antiviralSpermicidalanti-HIVEricinSBacillussubtilis

A1/3anti-Gram+SiamycinII

Streptomycesstrains

AA3891（链霉菌）Antiviralanti-HIVLactococcin972Lactococcuslactis

IPLA972（乳酸乳球菌）anti-Gram+MundticinATO6Enterococcusmundtii

ATO6（蒙氏肠球菌）anti-Gram+Piscicolin126/PiscicocinVia

Carnobacteriumpiscicola

JG126（栖鱼肉杆菌）anti-Gram+SakacinPLactobacillussake

Lb674anti-Gram+PediocinPA-1Pediococcusacidilactici

PAC-1.0anti-Gram+SpermicidalMesentericinY105Leuconostocmesenteroidesanti-Gram+CarnobacteriocinBM1Carnobacteriumpiscicola

LV17Banti-Gram+anti-Gram-CurvacinA/sakacinALactobacillussake

Lb706anti-Gram+PlanosporicinPlanomonosporaalbaanti-Gram+anti-Gram-GassericinALactobacillusgasseri

Lactobacillusreuteri

LA6anti-Gram+anti-Gram-CircularinAClostridiumbeijerinckii

ATCC25752anti-Gram+anti-Gram-Closticin574Clostridiumtyrobutyricum

ADRIAT932anti-Gram+anti-Gram-EnterocinE-760Enterococcusdurans/faecium/hiraeanti-Gram+anti-Gram-EnterocinAEnterococcusfaeciumanti-Gram+DivercinV41Carnobacteriumdivergens

V41anti-Gram+DivergicinM35Carnobacteriumdivergens

M35anti-Gram+Coagulin

Bacilluscoagulans

I-4anti-Gram+Listeriocin743AListeriainnocua

743anti-Gram+MundticinKSEnterococcusmundtii

NFRI7397anti-Gram+antiviralLeucocinCLactococcuslactis

MMFIanti-Gram+LactococcinMMFIILactococcuslactis

MMFIanti-Gram+SakacinG--anti-Gram+Plantaricin423Lactobacillusplantarum

423anti-Gram+PlantaricinC19

Lactobacillusplantarum

C19anti-Gram+EnterocinPEnterococcusfaecium

P13anti-Gram+Bacteriocin31Enterococcusfaecalisanti-Gram+CarnobacteriocinB1CarnobacteriumpiscicolaLV17Banti-Gram+anti-Gram-ButyrivibriocinAR10ButyrivibriumfibrisolvensAR10anti-Gram+anti-Gram-SubtilosinA

BacillussubtilisBacillusamyloliquefaciens,or

Bacillusatrophaeusanti-Gram+anti-GramantiviralSpermicidalEnterocinAS-48EnterococcusfaecalisS-48anti-Gram+anti-Gram-PlantaricinASM1Lactobacillusplantarum

A-1anti-Gram+Uberolysin

Streptococcusuberis42anti-Gram+anti-Gram-AcidocinBLactobacillusacidophilusM46anti-Gram+anti-Gram-CarnocinUI49Carnobacteriumpiscicola

UI49anti-Gram+anti-Gram-LicheninBacilluslicheniformisanti-Gram+anti-Gram-NisinQHalobacteriumstrainAS7092anti-Gram+Serracin-P23kDasubunitSerratiaplymuthicaanti-Gram+anti-Gram-Serracin-P43kDasubunitSerratiaplymuthicaanti-Gram+anti-Gram-VariacinKocuriavariansanti-Gram+anti-Gram-MutacinII

Streptococcusmutansanti-Gram+anti-Gram-MutacinIIIStreptococcusmutansanti-Gram+Mutacin,orMutacinB-Ny266Streptococcusmutansanti-Gram+anti-Gram-ThuricinS

Bacillusthuringiensisserovarentomocidusanti-Gram+AureocinA53Staphylococcusaureus

A53anti-Gram+SiamycinI

StreptomycesstrainAA6532Antiviralanti-HIVNP-06StreptomycesstrainAA6532Antiviralanti-HIVLactococcinG-a

LactococcuslactisLMGT2081anti-Gram+anti-Gram-LactococcinQLactococcuslactisQU4anti-Gram+LCI

Bacillussubtilisanti-Gram+anti-Gram-Enterocin1071EnterococcusfaecalisBFE1071anti-Gram+anti-Gram-PlantaricinSLactobacillusplantarumLPCO10anti-Gram+anti-Gram-AFP1StreptomycestendaeTu901（唐德链霉菌）antifungalPlantaricinNC8Lactobacillusplantarum

NC8anti-Gram+CarnocyclinACarnobacteriummaltaromaticumUAL307anti-Gram+LactacinFLactobacillusjohnsoniiVPI11088(Laf+)anti-Gram+BrochocinC

BrochothrixcampestrisATCC43754anti-Gram+Thermophilin13

Streptococcusthermophilusanti-Gram+ABP-118Lactobacillussalivariussubsp.salivariusUCC118anti-Gram+anti-Gram-SalivaricinPLactobacillussalivariusDPC6005anti-Gram+anti-Gram-MutacinIVStreptococcusmutansUA140anti-Gram+anti-Gram-Lactocin705LactobacilluscaseiCRL705anti-Gram+anti-Gram-CytolysinEnterococcusfaecali（粪肠球菌）anti-Gram+anti-Gram-MammaliancellsPlantaricinEFLactobacillusplantarum

C1anti-Gram+anti-Gram-PlantaricinJKLactobacillusplantarumC1anti-Gram+anti-Gram-EnterocinSE-K4Enterococcusfaeciumanti-Gram+AcidocinJ1132LactobacillusacidophilusJCM1132anti-Gram+anti-Gram-CurvaticinL442LactobacilluscurvatusL442anti-Gram+Bacteriocin32Enterococcusfaeciumanti-Gram+Bacteriocin43Enterococcusfaeciumanti-Gram+BacteriocinT8EnterococcusfaeciumT8anti-Gram+EnterocinBEnterococcusfaecium

T136;

Enterococcusfaecium

W3anti-Gram+AcidocinALactobacillusacidophilusTK9201anti-Gram+EnterocinQEnterococcusfaeciumL50anti-Gram+EnterocinRJ-11EnterococcusfaecalisRJ-11anti-Gram+EnterocinL50EnterococcusfaeciumL50anti-Gram+MR10AEnterococcusfaecalis

MRR10-3;

Enterococcusfaecalis

710Canti-Gram+anti-Gram-Lacticin3147LactococcuslactisDPC3147anti-Gram+SpermicidalSalivaricinAStreptococcussalivarius20P3anti-Gram+MicrocinE492

Klebsiellapneumoniae

RYC492anti-Gram-CancercellsHiracinJM79

EnterococcushiraeDCH5anti-Gram+Thermophilin9StreptococcusthermophilusLMD-9anti-Gram+PenocinA

PediococcuspentosaceusATCC25745anti-Gram+SalivaricinBStreptococcussalivariusK12anti-Gram+Lacticin481LactococcuslactisCNRZ481anti-Gram+BacteriocinJ46LactococcuslactisJ46anti-Gram+NukacinISK-1StaphylococcuswarneriISK-1,orStaphylococcussimulans3299anti-Gram+StreptococcinA-FF22streptococcusstrainFF22anti-Gram+LactocinSLactobacillussakeL45anti-Gram+MersacidinBacillussp.strainHILY-8554728anti-Gram+GramicidinB

Bacillusbrevisanti-Gram+anti-Gram-MicrocinC7Escherichiacolianti-Gram-MicrocinB17Escherichiacolianti-Gram-MicrocinLEscherichiacoliLR05anti-Gram-MicrocinMEscherichiacoliMC4100anti-Gram-MicrocinI47EscherichiacoliH47anti-Gram-Pep27Streptococcuspneumoniae（肺炎双球菌）anti-Gram+anti-Gram-

CancercellsMicrocin24E.coli2424anti-Gram-BTLB.subtilisB-TL2antifungalAdDLPAnaeromyxobacterdehalogenansantiparasiticAntimalarialPaenibacillinPaenibacilluspolymyxaanti-Gram+EpiderminStaphylococcusepidermidis

Tü3298anti-Gram+LichenicidinBacilluslicheniformis

ATCC14580orVK21anti-Gram+EnterocinXEnterococcusfaecium

KU-B5anti-Gram+ThuricinCD

BacillusthuringiensisDPC6431anti-Gram+DuramycinStreptoverticilliumgriseoverticillatumPA-48009anti-Gram+DuramycinBStreptoverticilliumstrainR2075anti-Gram+DuramycinCStreptomycesgriseoluteusR2107anti-Gram+Cinnamycin

StreptomycescinnamoneuscinnamoneusDSM40005anti-Gram+antifungalMammaliancellsPAMPPropionibacteriumjenseniiLMG3032anti-Gram+MichiganinAOmatopathogen,Clavibactermichiganensissubsp.michiganensisanti-Gram+Ipomicinsweetpotatopathogen,

Streptomycesipomoeaeanti-Gram+ActagardineActinoplanesgarbadinensisATCC31048and31049anti-Gram+Sublancin168Bacillussubtilis168anti-Gram+BovicinHJ50StreptococcusbovisHJ50anti-Gram+Thermophilin1277StreptococcusthermophilusSBT1277anti-Gram+PlantaricinWLactobacillusplantarumanti-Gram+StaphylococcinC55

StaphylococcusaureusC55anti-Gram+GalliderminStaphylococcusgallinarum(F16/P57)Tü3928anti-Gram+anti-Gram-Epilancin15X

Staphylococcusepidermidis

15X154anti-Gram+EpilancinK7

StaphylococcusepidermidisK7anti-Gram+Epicidin280StaphylococcusepidermidisBN280anti-Gram+MicrobisporicinA1Microbisporasp.,strain107891anti-Gram+SmbBStreptococcusmutansGS5anti-Gram+HaloduracinBacillushalodurans

C-125anti-Gram+CypemycinStreptomycessp.OH-4156anti-Gram+Plantazolicin

BacillusamyloliquefaciensFZB42anti-Gram+GarvicinML

Lactococcusgarvieae

DCC43anti-Gram+LactocyclicinQLactococcussp.strainQU12anti-Gram+anti-Gram-EntianinBacillussubtilisDSM15029Tanti-Gram+CapistruinBurkholderiathailandensis

E264anti-Gram-LariatinA

RhodococcusjostiiK01-B0171anti-Gram+LariatinBRhodococcusjostiiK01-B0171anti-Gram+GlycocinF

LactobacillusplantarumKW30anti-Gram+LeucocyclicinQLeuconostocmesenteroidesTK41401anti-Gram+anti-Gram-LaterosporulinBrevibacilluslaterosporusStrainGI-9anti-Gram+anti-Gram-antifungalGP-19Xenorhabdusbudapestensis

NMC-10anti-Gram+anti-Gram-antifungalEP-20Xenorhabdusbudapestensis

NMC-10antifungalGeobacillinIGeobacillusthermodenitrificans

NG80-2anti-Gram+GeobacillinIIGeobacillusthermodenitrificans

NG80-2anti-Gram+BacteriocinLS2

Lactobacillussalivarius

BGHO1anti-Gram+PatellamideLissoclinumpatella--Blp1Lactobacillussalivarius

BGHO1anti-Gram+Trichamide

Trichodesmiumerythraeum--EnterocinNKR-5-3DEnterococcusfaeciumanti-Gram+MicrocinSEscherichiacoli

G3/10anti-Gram-MicrocinPDIEscherichiacoli

25anti-Gram-m2163LactobacilluscaseiATCC334anti-Gram+m2386Lactobacilluscasei

ATCC334anti-Gram+SclerosinPseudomonassp.strainDF41anti-Gram+antifungalAstexin-1Asticcacaulisexcentricus

CB48anti-Gram-EnterocinAS-48RJEnterococcusfaecium

RJ16anti-Gram+anti-Gram-WarnericinRKStaphylococcuswarneri

RKanti-Gram-Mammaliancellsdelta-lysinIStaphylococcuswarneri

RKanti-Gram-MammaliancellPaenicidinA

Paenibacilluspolymyxa

NRRLB-30509anti-Gram+Antivirallectinscytovirincyanobacterium,

ScytonemavariumAntiviralanti-HIVCyanovirin-NcyanobacteriumAntiviralanti-HIVMicrocystisviridislectinblue-greenalgae,

MicrocystisviridisAntiviralanti-HIVPln149Lactobacillusplantarum

NRIC149anti-Gram+antifungalNAI-802

Actinoplanesspanti-Gram+BacCH91Staphylococcusaureus

CH-91anti-Gram+FusaricidinABacilluspolymyxa

KT-8anti-Gram+antifungalFusaricidinB

Bacilluspolymyxa

KT-8anti-Gram+antifungalFusaricidinBacilluspolymyxa

KT-8anti-Gram+antifungalFusaricidinDBacilluspolymyxa

KT-8anti-Gram+antifungalPeptideAN5-1Paenibacillusalvei

AN5anti-Gram+anti-Gram-NRWCBacillussubtilis

ATCC6633anti-Gram-PE1

Paenibacillusehimensis

B7anti-Gram+anti-Gram-antifungalDaptomycinStreptomycesroseosporus

NRRL11379anti-Gram+ColistinPaenibacilluspolymyxa

var.colistinusanti-Gram-,AntibiofilmSubtilosinA1Bacillussubtilisanti-Gram+AP02221Streptococcusmacedonicus

ACA-DC198anti-Gram+SubtilomycinBacillussubtilis

MMA7anti-Gram+anti-Gram-antifungalGramicidinSBacillusbrevisanti-Gram+anti-Gram-antifungalMammaliancellsEnterocinRM6Enterococcusfaecalis

OSY-RM6anti-Gram+lacticinQ

Lactococcuslactis

QU5anti-Gram+LsbALactococcuslactisanti-Gram+LsbBLactococcuslactisanti-Gram+BHT-BStreptococcusrattus

BHTanti-Gram+BHT-A1Streptococcusrattus

BHTanti-Gram+Enterocin7BEnterococcusfaecalis710C;anti-Gram+CmmAMP-I

plantpathogenanti-Gram+Brevifactin

amarineactinobacteriumanti-Gram+anti-Gram-antifungalAntioxidant,Salivaricin9Streptococcussalivarius

NU10anti-Gram+BacteriocinOR-7

Lactobacillussalivarius

strainNRRLB-30514anti-Gram-BacteriocinL-1077Lactobacillussalivarius

1077(NRRLB-50053)anti-Gram+anti-Gram-BactofencinALactobacillussalivarius

DPC6502anti-Gram+Plantaricin163Lactobacillusplantarum

163anti-Gram+anti-Gram-ChampacyclinmarinesedimentsoftheGotlandDeep(BalticSea)anti-Gram-antifungalFengycinB2BacillusthuringiensisantifungalBacFL31Enterococcusfaecium

FL31anti-Gram+PaenibacterinPaenibacillusthiaminolyticus

OSY-SEanti-Gram+anti-Gram-CerecidinA1Bacilluscereusanti-Gram+CerecidinA7

Bacilluscereusanti-Gram+MicrocinBEscherichiacolianti-Gram-BsaA2community-acquiredmethicillin-resistant

Santi-Gram+PepG1longStaphylococcusaureusanti-Gram+anti-Gram-PepG1short

Staphylococcusaureusanti-Gram+anti-Gram-AN5-2

Paenibacillusalvei

AN5anti-Gram+anti-Gram-Baceridinplant-associatedBacillusstrainanti-Gram+CancercellsLassomycin--anti-Gram+GageostatinABacillussubtilisanti-Gram+anti-Gram-antifungalCancercellsGageostatinBBacillussubtilisanti-Gram+anti-Gram-antifungalCancercellsGageostatinCBacillussubtilisanti-Gram+anti-Gram-antifungalCancercellsGageotetrinABacillussubtilisantifungalGageotetrinBBacillussubtilisantifungalGageotetrinC

BacillussubtilisantifungalThurincinH

Bacillusthuringiensis

SF361anti-Gram+Bac-GM100Brevibacillusbrevis

strainGM100anti-Gram+anti-Gram-antifungalAmylocyclicinBacillusamyloliquefaciens

FZB42anti-Gram+SonorensinBacillussonorensis

MT93anti-Gram+Perfrin

Clostridiumperfringensanti-Gram+SilStreptococcusiniae

SF1anti-Gram+GarvicinALactococcusgarvieae

21881anti-Gram+TaromycinAthemarineactinomyceteanti-Gram+MicrobisporicinA2

MicrobisporaATCCPTA-5024anti-Gram+Tolworthcin524Bacillusthuringiensis

subspanti-Gram+anti-Gram-PlantaricinZJ008

Lactobacillusplantarum

ZJ008anti-Gram+anti-Gram-PlantaricinZJ5Lactobacillusplantarum

ZJ5anti-Gram+anti-Gram-RLID12.1

Bacillussubtilis

RLID12.1anti-Gram+anti-Gram-antifungalLMWpeptide

Pediococcuspentosaceus

IE-3anti-Gram+anti-Gram-CarnolysinCarnobacteriummaltaromaticum

C2anti-Gram+MicrococcinP1

Staphylococcusepidermidis

strain115anti-Gram+antiparasiticAntimalarialS.amritsarensislipopeptideStreptomycesamritsarensisanti-Gram+Ala-6-fenycinBacillussp.strainP34anti-Gram+anti-Gram-antiviralEnterocinO16Enterococcusfaecalisanti-Gram+PseABacilluspseudomycoides

DSM12442anti-Gram+LabyrinthopeptinA2Actinomaduranamibiensisantiviral注：不同的颜色对应不同的抗性1.1.1来源于芽孢杆菌只抗革兰氏阴性菌（G-）的抗菌肽针对细菌中的抗菌肽，其中同源性最高的芽孢杆菌来源的抗菌肽更容易在芽孢杆菌表达系统中进行表达，故优先统计来源于芽孢杆菌只抗革兰氏阴性菌（G-）的抗菌肽（AMP）；共有3种，具体信息如下：a：NameNRWCSourceBacillussubtilis

ATCC6633Activityanti-Gram-3DStructureUnknownSequenceNRWCFAGDDTitleCharacterizationofanantimicrobialpeptideproducedbyBacillussubtilissubsp.spizeziniishowinginhibitoryactivityonHaemophilusparasuis【【】【】TeixeiraML,DallaRosaA,BrandelliA.CharacterizationofanantimicrobialpeptideproducedbyBacillussubtilissubsp.spizeziniishowinginhibitoryactivityonHaemophilusparasuis;Microbiology.2023May;159(Pt5):980-8.

Pub-Med;2023AuthorTeixeiraML,DallaRosaA,BrandelliA.2023ReferenceMicrobiology.2023May;159(Pt5):980-8.

Pub-Medb：NameColistinSourceBacilluspolymyxa（多粘芽孢杆菌）Activityanti-Gram-3DStructureUnknownSequenceKTKKKLLKKTTitlePolymyxin:anewchemotherapeuticagent【【】【】STANSLYPG,SHEPHERDRG,WHITEHJ.Polymyxin:anewchemotherapeuticagent;BullJohnsHopkinsHosp.1947Jul;81(1):43-54.

Pub-Med;1947AuthorSTANSLYPG,SHEPHERDRG,WHITEHJ.1947ReferenceBullJohnsHopkinsHosp.1947Jul;81(1):43-54.

Pub-Med.C：NameBacteriocinOR-7SourceLactobacillussalivarius（唾液乳杆菌）Activityanti-Gram-3DStructureUnknownSequenceKTYYGTNGVHCTKNSLWGKVRLKNMKYDQNTTYMGRLQDILLGWATGAFGKTFHTitleIsolationofaLactobacillussalivariusstrainandpurificationofitsbacteriocin,whichisinhibitorytoCampylobacterjejuniinthechickengastrointestinalsystem【【】【】SternNJ,SvetochEA,EruslanovBV,PerelyginVV,MitsevichEV,MitsevichIP,PokhilenkoVD,LevchukVP,SvetochOE,SealBS.IsolationofaLactobacillussalivariusstrainandpurificationofitsbacteriocin,whichisinhibitorytoCampylobacterjejuniinthechickengastrointestinalsystem;AntimicrobAgentsChemother.2006Sep;50(9):3111-6.Pub-Med;2006；AuthorSternNJ,SvetochEA,EruslanovBV,PerelyginVV,MitsevichEV,MitsevichIP,PokhilenkoVD,LevchukVP,SvetochOE,SealBS.2006ReferenceAntimicrobAgentsChemother.2006Sep;50(9):3111-6.Pub-Med1.1.2来源于芽孢杆菌抗真菌的的抗菌肽来源于芽孢杆菌抗真菌的抗菌肽（AMP）共有18种，其部分信息如下：A：NameLaterosporulinSourceBrevibacilluslaterosporus（侧孢短芽孢杆菌）trainGI-9Activityanti-Gram+，anti-Gram-，antifungal3DStructureUnknownSequenceACQCPDAISGWTHTDYQCHGLENKMYRHVYAICMNGTQVYCRTEWGSSCTitleIdentification,PurificationandCharacterizationofLaterosporulin,aNovelBacteriocinProducedbyBrevibacillussp.StrainGI-9【【】【】SinghPK,Chittpurna,Ashish,SharmaV,PatilPB,KorpoleS.Identification,PurificationandCharacterizationofLaterosporulin,aNovelBacteriocinProducedbyBrevibacillussp.StrainGI-9;PLoSOne.2023;7(3):e31498.

PubMed;2023AuthorSinghPK,Chittpurna,Ashish,SharmaV,PatilPB,KorpoleS.2023ReferencePLoSOne.2023;7(3):e31498.

PubMedB:NamePln149SourceLactobacillusplantarum（植物乳杆菌）isolatedfrompineappleActivityanti-Gram+,antifungal3DStructureUnknownSequenceYSLQMGATAIKQVKKLFKKKGGTitlePlantaricin-149,abacteriocinproducedbyLactobacillusplantarumNRIC149【【】【】KatoT,MatsudaT,OgawaE,OgawaH,KatoH,DoiU.Plantaricin-149,abacteriocinproducedbyLactobacillusplantarumNRIC149J.FermentationBioeng.1994;77(3):277-282;1994AuthorKatoT,MatsudaT,OgawaE,OgawaH,KatoH,DoiU.1994ReferenceFermentationBioeng.1994;77(3):277-282C:NameFusaricidinASourceBacilluspolymyxa

KT-8Activityanti-Gram+，anti-Gram-，antifungal3DStructureUnknownSequenceTVVTNATitleFusaricidinA,anewdepsipeptideantibioticproducedbyBacilluspolymyxaKT-8.Taxonomy,fermentation,isolation,structureelucidationandbiologicalactivity【【】【】KajimuraY,KanedaM.FusaricidinA,anewdepsipeptideantibioticproducedbyBacilluspolymyxaKT-8.Taxonomy,fermentation,isolation,structureelucidationandbiologicalactivity;JAntibiot(Tokyo).1996Feb;49(2):129-35.Pub-Med;1996AuthorKajimuraY,KanedaM.1996ReferenceJAntibiot(Tokyo).1996Feb;49(2):129-35.Pub-MedD:NameFusaricidinBSourceBacilluspolymyxa

KT-8Activityanti-Gram+,antifungal3DStructureUnknownSequenceTVVTQATitleFusaricidinsB,CandD,newdepsipeptideantibioticsproducedbyBacilluspolymyxaKT-8:isolation,structureelucidationandbiologicalactivity【【】【】KajimuraY,KanedaM.FusaricidinsB,CandD,newdepsipeptideantibioticsproducedbyBacilluspolymyxaKT-8:isolation,structureelucidationandbiologicalactivity;JAntibiot(Tokyo).1997Mar;50(3):220-8.Pub-Med;1997AuthorKajimuraY,KanedaM.1997ReferenceJAntibiot(Tokyo).1997Mar;50(3):220-8.Pub-MedE:NameFusaricidinCSourceBacilluspolymyxa

KT-8Activityanti-Gram+,antifungal3DStructureUnknownSequenceTVYTNATitleFusaricidinsB,CandD,newdepsipeptideantibioticsproducedbyBacilluspolymyxaKT-8:isolation,structureelucidationandbiologicalactivity【【】【】KajimuraY,KanedaM.FusaricidinsB,CandD,newdepsipeptideantibioticsproducedbyBacilluspolymyxaKT-8:isolation,structureelucidationandbiologicalactivity;JAntibiot(Tokyo).1997Mar;50(3):220-8.Pub-Med;1997AuthorKajimuraY,KanedaM.1997ReferenceJAntibiot(Tokyo).1997Mar;50(3):220-8.Pub-MedF:NameFusaricidinDSourceBacilluspolymyxa

KT-8Activityanti-Gram+，antifungal3DStructureUnknownSequenceTVYTQATitleFusaricidinsB,CandD,newdepsipeptideantibioticsproducedbyBacilluspolymyxaKT-8:isolation,structureelucidationandbiologicalactivity【【】【】KajimuraY,KanedaM.FusaricidinsB,CandD,newdepsipeptideantibioticsproducedbyBacilluspolymyxaKT-8:isolation,structureelucidationandbiologicalactivity;JAntibiot(Tokyo).1997Mar;50(3):220-8.Pub-Med;1997AuthorKajimuraY,KanedaM.1997ReferenceJAntibiot(Tokyo).1997Mar;50(3):220-8.Pub-MedG:NamePE1SourcePaenibacillusehimensis

B7Activityanti-Gram+，anti-Gram-，antifungal3DStructureUnknownSequenceKIKFLKVLTTitleIsolationandpartialcharacterizationofcycliclipopeptideantibioticsproducedbyPaenibacillusehimensisB7【【】【】HuangZ,HuY,ShouL,SongM.IsolationandpartialcharacterizationofcycliclipopeptideantibioticsproducedbyPaenibacillusehimensisB7;BMCMicrobiol.2023Apr17;13(1):87.

Pub-Med;2023AuthorHuangZ,HuY,ShouL,SongM.2023ReferenceBMCMicrobiol.2023Apr17;13(1):87.

Pub-MedH：NameSubtilomycinSourceBacillussubtilis

MMA7Activityanti-Gram+&Gram-,antifungal3DStructureUnknownSequenceTWATIGKTIVQSVKKCRTFTCGCSLGSCSNCNTitleSubtilomycin:ANewLantibioticfromBacillussubtilisStrainMMA7IsolatedfromtheMarineSpongeHaliclonasimulans【【】【】PhelanRW,BarretM,CotterPD,O'ConnorPM,ChenR,MorrisseyJP,DobsonAD,O'GaraF,BarbosaTM.Subtilomycin:ANewLantibioticfromBacillussubtilisStrainMMA7IsolatedfromtheMarineSpongeHaliclonasimulans;MarDrugs.2023Jun3;11(6):1878-98.Pub-Med;2023AuthorPhelanRW,BarretM,CotterPD,O'ConnorPM,ChenR,MorrisseyJP,DobsonAD,O'GaraF,BarbosaTM.2023ReferenceMarDrugs.2023Jun3;11(6):1878-98.Pub-MedI:NameGramicidinSSourceBacillusbrevisActivityanti-Gram+&Gram-，antifungal，Mammaliancells3DStructureUnknownSequenceVKLFPVKLFPTitleGrammicidinSanditsuseinthetreatmentofinfectedwounds【【】【】GauseGF,BrazhnikovaMG.GrammicidinSanditsuseinthetreatmentofinfectedwounds；Nature1944;154:703.

PDF.；1944AuthorGauseGF,BrazhnikovaMG.1944ReferenceNature1944;154:703.

PDF.J:NameFengycinB2

SourceBacillusthuringiensis(苏云金芽孢杆菌)Activityantifungal3DStructureUnknownSequenceEKYTEVPEYVTitlePurification,biochemicalcharacterizationandself-assembledstructureofafengycin-likeantifungalpeptidefromBacillusthuringiensisstrainSM1【【】【】RoyA,MahataD,PaulD,KorpoleS,FrancoOL,MandalSM.Purification,biochemicalcharacterizationandself-assembledstructureofafengycin-likeantifungalpeptidefromBacillusthuringiensisstrainSM1;FrontMicrobiol.2023Nov21;4:332.

PubMed;2023AuthorRoyA,MahataD,PaulD,KorpoleS,FrancoOL,MandalSM.2023ReferenceFrontMicrobiol.2023Nov21;4:332.

PubMedK:NameGageostatinASourceBacillussubtilisActivityanti-Gram+&Gram-,antifungal,Cancercells3DStructureUnknownSequenceELLVDLLTitleGageostatinsA-C,antimicrobiallinearlipopeptidesfromamarineBacillussubtilis【【】【】TareqFS,LeeMA,LeeHS,LeeJS,LeeYJ,ShinHJ.GageostatinsA-C,antimicrobiallinearlipopeptidesfromamarineBacillussubtilis;MarDrugs.2023Jan31;12(2):871-85.doi:10.3390/md12023871.PubMed;2023AuthorTareqFS,LeeMA,LeeHS,LeeJS,LeeYJ,ShinHJ.2023ReferenceMarDrugs.2023Jan31;12(2):871-85.doi:10.3390/md12023871.PubMedL：NameGageostatinBSourceBacillussubtilisActivityanti-Gram+&Gram-,antifungal,Cancercells3DStructureUnknownSequenceELLVDLLTitleGageostatinsA-C,antimicrobiallinearlipopeptidesfromamarineBacillussubtilis【【】【】TareqFS,LeeMA,LeeHS,LeeJS,LeeYJ,ShinHJ.GageostatinsA-C,antimicrobiallinearlipopeptidesfromamarineBacillussubtilis;MarDrugs.2023Jan31;12(2):871-85.doi:10.3390/md12023871.PubMed;2023AuthorTareqFS,LeeMA,LeeHS,LeeJS,LeeYJ,ShinHJ.2023ReferenceMarDrugs.2023Jan31;12(2):871-85.doi:10.3390/md12023871.PubMedM：NameGageostatinCSourceBacillussubtilisActivityanti-Gram+&Gram-,antifungal,Cancercells3DStructureUnknownSequenceELLVDLLTitleGageostatinsA-C,antimicrobiallinearlipopeptidesfromamarineBacillussubtilis【【】【】TareqFS,LeeMA,LeeHS,LeeJS,LeeYJ,ShinHJ.GageostatinsA-C,antimicrobiallinearlipopeptidesfromamarineBacillussubtilis；MarDrugs.2023Jan31;12(2):871-85.doi:10.3390/md12023871.PubMed;2023AuthorTareqFS,LeeMA,LeeHS,LeeJS,LeeYJ,ShinHJ.2023ReferenceMarDrugs.2023Jan31;12(2):871-85.doi:10.3390/md12023871.PubMedN:NameBac-GM100

SourceBrevibacillusbrevis

strainGM100Activityanti-Gram+&Gram-，antifungal3DStructureUnknownSequenceDWTFANWSCLVCDDCSVNLTVTitlePurificationandbiochemicalcharacterizationofahighlythermostablebacteriocinisolatedfromBrevibacillusbrevisstrainGM100【【】【】GhadbaneM,HarzallahD,LaribiAI,JaouadiB,BelhadjH.PurificationandbiochemicalcharacterizationofahighlythermostablebacteriocinisolatedfromBrevibacillusbrevisstrainGM100;BiosciBiotechnolBiochem.2023;77(1):151-60.PubMed;2023AuthorGhadbaneM,HarzallahD,LaribiAI,JaouadiB,BelhadjH.2023ReferenceBiosciBiotechnolBiochem.2023;77(1):151-60.PubMed来源于芽孢杆菌抗真菌同时抗革兰氏阳性菌（G+）的抗菌肽