IWR-1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrarieswww.MedChemEIWR-1Cat.No.:HY-12238CASNo.:1127442-82-3分⼦式:C₂₅H₁₉N₃O₃分⼦量:409.44作⽤靶点:Wnt作⽤通路:StemCell/Wnt储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥46mg/mL(112.35mM)扫描⼆维码，*"≥"meanssoluble,butsaturationunknown.运⽤溶解⽅案计算器获得适合您实验体系的溶解⽅案MassSolvent1mg5mg10mgConcentration制备储备液1mM2.4424mL12.2118mL24.4236mL5mM0.4885mL2.4424mL4.8847mL10mM0.2442mL1.2212mL2.4424mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶1.请依序添加每种溶剂：10%DMSO40%PEG3005%Tween-8045%salineSolubility:≥2.5mg/mL(6.11mM);Clearsolution此⽅案可获得≥2.5mg/mL(6.11mM，饱和度未知)的澄溶液。以1mL⼯作液为例，取100μL25.0mg/mL的澄DMSO储备液加到400μLPEG300中，混合均匀；向上述体系中加⼊50μLTween-80，混合均匀；然后继续加⼊450μL⽣理盐⽔定容⾄1mL。1/3www.MedChemEwww.MedChemE2.请依序添加每种溶剂：10%DMSO90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(6.11mM);Clearsolution此⽅案可获得≥2.5mg/mL(6.11mM，饱和度未知)的澄溶液。以1mL⼯作液为例，取100μL25.0mg/mL的澄DMSO储备液加到900μL20%的SBE-β-CD⽣理盐⽔⽔溶3.液中，混合均匀。请依序添加每种溶剂：10%DMSO90%cornoilSolubility:≥2.5mg/mL(6.11mM);Clearsolution此⽅案可获得≥2.5mg/mL(6.11mM，饱和度未知)的澄溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL25.0mg/mL的澄DMSO储备液加到900μL⽟⽶油中，混合均匀。BIOLOGICALACTIVITY⽣物活性IWR-1端锚聚合酶抑制剂，抑制Wnt/β-catenin信号传导途径。IC50&TargetIC50:180nM(Wnt)体外研究BothIWR-1andXAV939actasreversibleWntpathwayinhibitorsandexhibitsimilarpharmacologicaleffectsinvitro.IWR-1exertsitseffectviainteractionwithAxin,whileXAV939bindsTNKSdirectly[1].IWR-1(10μM)inducesstabilizationofβ-catenindisruptioncomplex.IWR-1(10μM)isaddedtothemediumtogetherwithMIF,thesizeofcellcoloniesisextremelydecreased,andthatindicatesthepromotingeffectofMIFonNSPCproliferationisinhibitedbyIWR-1inanyMIFconcentrationgroup.2,5and10μMofIWR-1significantlyinhibitstheproliferationofNSPCdose-dependently.IWR-1inhibitesthepromotingeffectofMIFonNSPCdifferentiationtoneuronlineage[2].IWR-1treatmentinthepresenceofmaximalstimulatorydoseofFSH(0.5ng/mL)resultsinadosedependentinhibitionofthestimulatoryeffectofFSHwith>75%inhibitionobservedatthemaximalinhibitorydoseofIWR-1(1µM).IWR-1treatmentpartiallyreversestheFSH-inducedinhibitionofgranulosacellCARTPTmRNAexpression[3].户使⽤本产品发表的科研⽂献•CellBiosci.2019Jun14;9:48.•iScience.2019Sep27;19:1248-1259.•CancerCellInt.2019Nov27;19:314.•JOrthopTransl.2020Mar28;23:29-37.•OncolRep.2018Aug;40(2):877-886.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LuJ,etal.Structure-activityrelationshipstudiesofsmall-moleculeinhibitorsofWntresponse.BioorgMedChemLett.2009Jul15;19(14):3825-7.[2].ZhangX,etal.Macrophagemigrationinhibitoryfactorpromotesproliferationandneuronaldifferentiationofneuralstem/precursorcells2/3www.MedChemEwww.MedChemEthroughWnt/β-cateninsignalpathway.IntJBiolSci.2013Nov28;9(10):1108-20.[3].GuptaPS,etal.RegulationandRegulatoryRoleofWNTSignalinginPotentiatingFSHActionduringBovineDominantFollicleSelection.PLoSOne.2014Jun17;9(6):e100201.McePdfHeight关注MCE中国公众号，

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