简介Waters-UPLC故障排除课件

简介Waters-UPLC故障排除课件WatersUPLCTroubleshootingTraining

WatersUPLCTroubleshootingTrAgendaACQUITYUPLCFamily：UPLC&H-ClassWatersUPLCSystemUPLCSolventManagement

(BSM)UPLCSampleManagement

(SM)WatersACQUITY

H-ClassSystemQuaternarySolventManager(QSM)SampleManager-FlowThroughNeedle(SM-FTN)WatersUPLCDetectorTroubleshootingTrainingWatersUPLCPDAandPDAeλTUVFLRELSDOpenDiscussion

AgendaACQUITYUPLCFamily：UPLACQUITYUPLCFamilyACQUITYUPLCFamilyACQUITYUPLCSystemACQUITYUPLCSystemACQUITYUPLCSystemDetectors:Opticaland/orMassSpecTunableUVorPhotodiodeArrayOptimizedflowcellforUPLCHighspeeddatasamplingColumnManager:InnovativepivotdesignPositionscolumntodetectorE-CordconnectionSampleManager:LowdispersionXYZZ’FormatFastcycletimesLowcarryoverPlatesand/orvialsOptionalSampleOrganizerBinarySolventManager:HighpressureblendingBinarygradientsFoursolventchoicesOn-linedegassingLowdispersiondesignUPLCpressurecapabilitiesSampleOrganizer:

(option)Expandscapacity(22/15/8)ShuttlesplatefeedHeated/chilledSystemConsiderations:SmallFootprintRedesignedtubingandfittingsConsolidatedwastemanagementIntegratedsystemdiagnosticsConnectionsInsightremotediagnosticsACQUITYUPLCSystemDetectors:CA1A2B1B2WasteFluidics

andConnectionsTubingmanagementChromatographicsolvents:A1/A2,B1/B2TwoSampleManagerwashsolventsSealWashCommonfittingsandtoolsforusersColumninlets/outletsandfittingsFingertight,¼turn(use1/4”wrench)Dedicatedtubing3piececompressionscrewandferruleSmallfootprint–13½”wide24.25”withSampleOrganizerA1A2B1B2WasteFluidicsandCoUPLC

SolventManagerUPLC

SolventManagerACQUITYUPLCSystem

SolventDelivery

15KpsiperformanceAccurateandpreciseflowratesAccurateandprecisecompositionLowdelayvolumeforgradientapplications<120µLSystemVolumeLongtermreliabilityACQUITYUPLCSystem

SolventDBinarySolventManagerComponentsFilter/Mixer/TeeAssembly“A”PumpAccumulator“A”PumpSolventSelectValve“B”PumpSolventSelectValve“A”PumpPrimary“B”PumpPrimary“B”PumpAccumulatorSixChannelDegasserBinarySolventManagerComponeIntelligentIntakeCheckValvesIntelligentIntakeCheckValveACQUITYUPLCSystem

FromSerialtoParallelFlowTwoindependentlydrivenprimaryandaccumulatorpistondrivesSeparatedrivemotorsTwotransducersTwoinletcheckvalvesFourSolventsCapabilityA1orA2andB1orB2TwosolventspergradientSixChannelDegasser4chromatographiceluents2solventsforSampleManagerStrong&weakwash“B”“A”B2B1A2SolventA1ACQUITYUPLCSystem

FromSeriBinarySolventManager

InteractiveDisplayBinarySolventManager

InteraRecommendedSolventsListACQUITYUPLCSystemrecommendedSolvents

Methanol Methanol/watermixtures Water Acetonitrile/watermixtures Acetonitrile(ACN) Isopropanol(IPA)Samplediluents(inadditiontothesolventslistedabove)

Dimethylsulfoxide(DMSO) Dimethylformamide(DMF)Additives/Modifiers

0.2%formicacid 0.1%trifluoroaceticacid(TFA) 0.1%triethylamine(TEA) 0.1%hexafluorobutericacid 10mMphosphatebuffer 10mMammoniumbicarbonate 50mMammoniumhydroxide 50mMammoniumacetate 0.1%Ethylenediaminetetraaceticacid(EDTA)Cleaners

Phosphoricacid(=30%) Sodiumhydroxide(=1M)ACQUITYUPLCSystemnon-recommendedSolvents

Methylene-Chloride Chloroform Strongacids>5% EthylAcetate Toluene Chlorinatedsolvents:(Trichlorobenzene)

RecommendedSolventsListACQUIUPLC

SampleManagerUPLC

SampleManagerSampleManagerFluidicsTray Pressure TransducerValveManifold

DriveGears

WashSyringesWeakWashStrongWashMeteringSyringeDriveAssemblySampleManagerFluidicsTray MeteringSyringeValve3WayRotaryValveMeteringSyringeAssemblySampleSyringe50uLvolumePN410001348100µLvolumePN700002570250µLvolumePN410001347forusewith50µLloopMeteringSyringeValveMeteringColumnHeatingCompartmentColumnHeatingCompartmentACQUITYIn-LineFilter19ACQUITYIn-LineFilter19ColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnHeatingColumnHeatingSampleManagerFlowPathSampleManagerFlowPathAvailableLoopsDescription P/N Status 1µLLoop 430001516 Optional2µLLoop 430001264 Optional5µLLoop 430001233 Optional10µLLoop 430001326 Standard20µLLoop 430001320 Optional50µLLoop 430001325 Optional100µLLoop 170001792 (Kit)Optional250µLLoop 176001780 (Kit)OptionalAvailableLoopsDescription P/VolumeConfigurationMenuBeforerunningtheCharacterizeNeedleandLoopVolumefunction,makesurethepropervolumesareconfiguredintheVolumesConfigurationmenu.TheabovevaluesarestandardforcurrentlyshippingACQUITYsystem.VolumeConfigurationMenuBeforRecommendedInjectionVolumes,

LoopSizesandInjectionModesRecommendedPLNOinjectionvolume20%to75%ofloopvolumeRecommendedPartialLoopInjection20%to50%ofloopvolumeRecommendedInjectionVolumes,ACQUITYH-ClassSystemQSMQuaternarySolventManagerSM-FTNSampleManager-FlowThroughNeedleCH-AHighTemperatureColumnHeaterActivePre-HeatingDetectorsTUV,PDAePDA,FLR,ELSDACQUITYH-ClassSystemQSMSM-FTTheACQUITYH-ClassSystemconsistsofthefollowing:QuaternarySolventManager(QSM)FoursolventlowpressuregradientmixingSupportsUPLCPressuresLowgradientdelayvolumeSM-FTN(SampleManager-FlowThroughNeedle)Oneinjectionmode-directinjecttypeMuchsimpler-JustspecifyinjectionvolumeExcellentrecoveryandprecisionCH-AHightemperaturecolumnheaterwithactivepre-heatingACQUITYH-ClassSystemTheACQUITYH-ClassSystemconQSMFluidics-FrontViewPrimaryHeadAccumulatorHeadVentValve100ulMixerGradientProportioningValvePairsLowPressureManifoldDegasserChambersSealWashPumpQSMFluidics-FrontViewPrimaryQSMConsoleTopLevelMenuQSMConsoleTopLevelMenuACQUITYH-ClassSystemACQUITYH-ClassSystem简介Waters-UPLC故障排除课件SMDIFrontPanelFluidicsMeteringSyringeValveCheckValveandUnionInjectValveWashSolventInletLine(notdegassed)LeakSensorConnectionsSampleMeteringSyringeSMDIFrontPanelFluidicsMeterSDMIInjectValveConnectionsToCheckValveToCH-AandColumnStationarySideTubeFromPumpToPressureTransducerToSampleNeedleSDMIInjectValveConnectionsTSampleCompartmentPowerandStatusLEDsPlateSelectorSwitchRotaryTray(PlateHolder)MagneticDoorSensorPowerSwitchSampleCompartmentPowerandStSM-FTNInteractiveDisplayMenuSM-FTNInteractiveDisplayMenCH-AImprovedDoorGasketAddedTubingGasket(topandbottom)For3rdpartyMassSpecatleftsideofACQUITYStack.AllowsuseofexistingPassivePre-heaterNowirestoconnectActivePre-heaterCH-AImprovedDoorGasketAddedInstallingtheActivePre-HeaterInstallingtheActivePre-HeatGoldPlatedCompressionScrewPEEKFerruleStainlessSteelCapNutStainlessSteelTubing(withbrazedoncollet)InstallingtheActivePre-HeaterGoldPlatedCompressionScrewPUPLC

DetectorsUPLC

DetectorsDetectorsSupportsallcurrentACQUITYdetectorsPDAandPDAeλTUVFLRELSDDetectorsSupportsallcurrentACQUITY

UPLC

PDAACQUITYUPLCPDAACQUITY

UPLC

PDAPMkitsACQUITYUPLCPDAPMkitsACQUITY

UPLC

PDA

OpticsAssembly

andSpectrumPPT课件ACQUITYUPLCPDAOpticsAssembACQUITY

UPLC

TUVPPT课件ACQUITYUPLCTUVPPT课件ACQUITY

UPLC

TUV

OpticsAssemblyPPT课件ACQUITYUPLCTUVOpticsAssembACQUITY

UPLC

TUV

OpticsAssemblyPPT课件ACQUITYUPLCTUVOpticsAssembACQUITY

UPLC

FLRACQUITYUPLCFLRACQUITY

UPLC

FLR

OpticsAssemblyPPT课件ACQUITYUPLCFLROpticsAssembACQUITY

UPLC

ELSDPPT课件ACQUITYUPLCELSDPPT课件Questions?PPT课件Questions?PPT课件简介Waters-UPLC故障排除课件简介Waters-UPLC故障排除课件WatersUPLCTroubleshootingTraining

WatersUPLCTroubleshootingTrAgendaACQUITYUPLCFamily：UPLC&H-ClassWatersUPLCSystemUPLCSolventManagement

(BSM)UPLCSampleManagement

(SM)WatersACQUITY

H-ClassSystemQuaternarySolventManager(QSM)SampleManager-FlowThroughNeedle(SM-FTN)WatersUPLCDetectorTroubleshootingTrainingWatersUPLCPDAandPDAeλTUVFLRELSDOpenDiscussion

AgendaACQUITYUPLCFamily：UPLACQUITYUPLCFamilyACQUITYUPLCFamilyACQUITYUPLCSystemACQUITYUPLCSystemACQUITYUPLCSystemDetectors:Opticaland/orMassSpecTunableUVorPhotodiodeArrayOptimizedflowcellforUPLCHighspeeddatasamplingColumnManager:InnovativepivotdesignPositionscolumntodetectorE-CordconnectionSampleManager:LowdispersionXYZZ’FormatFastcycletimesLowcarryoverPlatesand/orvialsOptionalSampleOrganizerBinarySolventManager:HighpressureblendingBinarygradientsFoursolventchoicesOn-linedegassingLowdispersiondesignUPLCpressurecapabilitiesSampleOrganizer:

(option)Expandscapacity(22/15/8)ShuttlesplatefeedHeated/chilledSystemConsiderations:SmallFootprintRedesignedtubingandfittingsConsolidatedwastemanagementIntegratedsystemdiagnosticsConnectionsInsightremotediagnosticsACQUITYUPLCSystemDetectors:CA1A2B1B2WasteFluidics

andConnectionsTubingmanagementChromatographicsolvents:A1/A2,B1/B2TwoSampleManagerwashsolventsSealWashCommonfittingsandtoolsforusersColumninlets/outletsandfittingsFingertight,¼turn(use1/4”wrench)Dedicatedtubing3piececompressionscrewandferruleSmallfootprint–13½”wide24.25”withSampleOrganizerA1A2B1B2WasteFluidicsandCoUPLC

SolventManagerUPLC

SolventManagerACQUITYUPLCSystem

SolventDelivery

15KpsiperformanceAccurateandpreciseflowratesAccurateandprecisecompositionLowdelayvolumeforgradientapplications<120µLSystemVolumeLongtermreliabilityACQUITYUPLCSystem

SolventDBinarySolventManagerComponentsFilter/Mixer/TeeAssembly“A”PumpAccumulator“A”PumpSolventSelectValve“B”PumpSolventSelectValve“A”PumpPrimary“B”PumpPrimary“B”PumpAccumulatorSixChannelDegasserBinarySolventManagerComponeIntelligentIntakeCheckValvesIntelligentIntakeCheckValveACQUITYUPLCSystem

FromSerialtoParallelFlowTwoindependentlydrivenprimaryandaccumulatorpistondrivesSeparatedrivemotorsTwotransducersTwoinletcheckvalvesFourSolventsCapabilityA1orA2andB1orB2TwosolventspergradientSixChannelDegasser4chromatographiceluents2solventsforSampleManagerStrong&weakwash“B”“A”B2B1A2SolventA1ACQUITYUPLCSystem

FromSeriBinarySolventManager

InteractiveDisplayBinarySolventManager

InteraRecommendedSolventsListACQUITYUPLCSystemrecommendedSolvents

Methanol Methanol/watermixtures Water Acetonitrile/watermixtures Acetonitrile(ACN) Isopropanol(IPA)Samplediluents(inadditiontothesolventslistedabove)

Dimethylsulfoxide(DMSO) Dimethylformamide(DMF)Additives/Modifiers

0.2%formicacid 0.1%trifluoroaceticacid(TFA) 0.1%triethylamine(TEA) 0.1%hexafluorobutericacid 10mMphosphatebuffer 10mMammoniumbicarbonate 50mMammoniumhydroxide 50mMammoniumacetate 0.1%Ethylenediaminetetraaceticacid(EDTA)Cleaners

Phosphoricacid(=30%) Sodiumhydroxide(=1M)ACQUITYUPLCSystemnon-recommendedSolvents

Methylene-Chloride Chloroform Strongacids>5% EthylAcetate Toluene Chlorinatedsolvents:(Trichlorobenzene)

RecommendedSolventsListACQUIUPLC

SampleManagerUPLC

SampleManagerSampleManagerFluidicsTray Pressure TransducerValveManifold

DriveGears

WashSyringesWeakWashStrongWashMeteringSyringeDriveAssemblySampleManagerFluidicsTray MeteringSyringeValve3WayRotaryValveMeteringSyringeAssemblySampleSyringe50uLvolumePN410001348100µLvolumePN700002570250µLvolumePN410001347forusewith50µLloopMeteringSyringeValveMeteringColumnHeatingCompartmentColumnHeatingCompartmentACQUITYIn-LineFilter71ACQUITYIn-LineFilter19ColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnHeatingColumnHeatingSampleManagerFlowPathSampleManagerFlowPathAvailableLoopsDescription P/N Status 1µLLoop 430001516 Optional2µLLoop 430001264 Optional5µLLoop 430001233 Optional10µLLoop 430001326 Standard20µLLoop 430001320 Optional50µLLoop 430001325 Optional100µLLoop 170001792 (Kit)Optional250µLLoop 176001780 (Kit)OptionalAvailableLoopsDescription P/VolumeConfigurationMenuBeforerunningtheCharacterizeNeedleandLoopVolumefunction,makesurethepropervolumesareconfiguredintheVolumesConfigurationmenu.TheabovevaluesarestandardforcurrentlyshippingACQUITYsystem.VolumeConfigurationMenuBeforRecommendedInjectionVolumes,

LoopSizesandInjectionModesRecommendedPLNOinjectionvolume20%to75%ofloopvolumeRecommendedPartialLoopInjection20%to50%ofloopvolumeRecommendedInjectionVolumes,ACQUITYH-ClassSystemQSMQuaternarySolventManagerSM-FTNSampleManager-FlowThroughNeedleCH-AHighTemperatureColumnHeaterActivePre-HeatingDetectorsTUV,PDAePDA,FLR,ELSDACQUITYH-ClassSystemQSMSM-FTTheACQUITYH-ClassSystemconsistsofthefollowing:QuaternarySolventManager(QSM)FoursolventlowpressuregradientmixingSupportsUPLCPressuresLowgradientdelayvolumeSM-FTN(SampleManager-FlowThroughNeedle)Oneinjectionmode-directinjecttypeMuchsimpler-JustspecifyinjectionvolumeExcellentrecoveryandprecisionCH-AHightemperaturecolumnheaterwithactivepre-heatingACQUITYH-ClassSystemTheACQUITYH-ClassSystemconQSMFluidics-FrontViewPrimaryHeadAccumulatorHeadVentValve100ulMixerGradientProportioningValvePairsLowPressureManifoldDegasserChambersSealWashPumpQSMFluidics-FrontViewPrimaryQSMConsoleTopLevelMenuQSMConsoleTopLevelMenuACQUITYH-ClassSystemACQUITYH-ClassSystem简介Waters-UPLC故障排除课件SMDIFrontPanelFluidicsMeteringSyringeValveCheckValveandUnionInjectValveWashSolventInletLine(notdegassed)LeakSensorConnectionsSampleMeteringSyringeSMDI