Sulfosuccinimidyl-oleate-sodium-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESulfosuccinimidyl oleate sodiumCat. No.: HY-112847A分式: CHNNaOS分量: 481.58作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 62.5 mg/mL (129.78 mM; Need ultrasonic)H2O : 40

2、% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (4.32 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.08 mg/mL (4.32 mM); Clear solutionBIOLOGICAL ACTIVITY1/3 Master of Small Molecules 您边的抑制剂师www.MedChemE物活性 Sulfosuccinimidyl oleate sodium种长链脂酸，抑制脂酸向细胞转运。Sulfosuccinimidyl o

3、leate 结合胶质细胞表上的 CD36受体。具有抗炎作 1。体外研究 Sulfosuccinimidyl oleate (20 M and 50 M, 24 hours) alone does not alter the cellular viability. Exposure to100 ng/ml LPS+5 ng/mL IFN modestly, yet significantly reduces the viability of the BV2 cells. Co-treatmentwith Sulfosuccinimidyl oleate prevents the LPS+IFN-

4、induced reduction in the cell viability 1.Sulfosuccinimidyl oleate (50 M, 24 hours) co-treatment significantly reduces the LPS+IFN-inducedexpression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFN-inducedupregulation in the phosphorylated form of the p38, which is

5、 prevented by co-treatment with Sulfosuccinimidyloleate (50 M, 24 hours) 1.Cell Viability Assay 1Cell Line: BV2 cellsConcentration: 20 M and 50 MIncubation Time: 24 hoursResult: Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5ng/mL IFN significantly reduce

6、d the viability of BV2 cells while simultaneous treatmentwith Sulfosuccinimidyl oleate prevented it.Western Blot Analysis 1Cell Line: BV2 cellsConcentration: 50 MIncubation Time: 24 hoursResult: Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38.体内研究 Sulfosuccinimidyl oleate (50 mg/kg;

7、 administered once by single oral gavage) significantly reduces the corticalischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. Inaddition, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke 1.Animal Model: 4-mont

8、h-old male BALB/cABom mice with pMCAo model 1Dosage: 50 mg/kgAdministration: Administered once by single oral gavageResult: Reduced brain damage following ischemia. Attenuated infarct size.REFERENCES2/3 Master of Small Molecules 您边的抑制剂师www.MedChemE1. Dhungana H, et al. Sulfosuccinimidyl oleate sodium is neuroprotective and alleviates stroke-induced neuroinflammation. JNeuroinflammation. 2017 Dec 4;14(1):237.McePdfHeightCaution: Product has not been fully validated for medical applic