常用荧光染料的激发及发射波长

1、 /16荧光素名称激发波长(nm)发射波长(nm)备注1,5IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein(5-FAM)4925185-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-FAM(5-Carboxyfluorescein)4925185-HAT(Hydro

2、xyTryptamine)370-415520-5405-HydroxyTryptamine(HAT)370-415520-5405-ROX(carboxy-X-rhodamine)5785676045915-TAMRA(5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine6G5185436-CR6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-AminoactinomycinD(7-AAD)5466477-Hydroxy-4-methylcoumarin360449,455

3、9-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445AcidFuchsin540630ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474AcridineOrange+DNA502526AcridineOrange+RNA460650AcridineOrange,bothDNA&RNA440-480520-650AcridineRed455-600560-680AcridineYellow470550Acriflavin436520AcriflavinFeulgenSITSA3

4、55-425460Aequorin(Photoprotein)466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFPAlexaFluor350346342442441AlexaFluor430431540AlexaFluor488495,492519,520AlexaFluor532531,532553,554AlexaFluor546556,557572,573AlexaFluor568577,578603AlexaFluor594590,594617,618AlexaFluor633

5、632650AlexaFluor647647666AlexaFluor660668698AlexaFluor680679702AlizarinComplexon530-560,580580,624645AlizarinRed530-560580Allophycocyanin(APC)630,645655,660AMC,AMCA-S345445AMCA(Aminomethylcoumarin)345347425444AMCA-X353442AminoactinomycinD555655Aminocoumarin346350442445Aminomethylcoumarin(AMCA)345347

6、425444AnilinBlue600Anthrocylstearate360-381446APC(Allophycocyanin)630,645655,660APC-Cy7625-650755APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505AstrazonBrilliantRed4G500585AstrazonOrangeR470540AstrazonRed6B520595AstrazonYellow7GLL450480Atabrine436490ATTO-TAGtmCBQCA465560ATTO-TAGtmFQ4865

7、91Auramine460550AurophosphineG450580Aurophosphine450-490515BAO9(Bisaminophenyloxadiazole)365395BCECF(highpH)492,503520,528BCECF(lowpH)482520BerberineSulphate430550BetaLactamase409447,520BFPblueshiftedGFP(Y66H)381,382,445,447,blueshiftedGFPBlueFluorescentProtein383448(Y66H)BlueFluorescentProteinBFP/G

8、FPFRETBimane398490Bisbenzamide360461Bisbenzimide(Hoechst)360461bis-BTC=RatioDye,Zn2+455/405529/505RatioDye,Zn2+BlancophorFFG390470BlancophorSV370435BOBO-1462481BOBO-3570602Bodipy492/515490515Bodipy493/503533549Bodipy500/510509515Bodipy505/515502510Bodipy530/550528547Bodipy542/563543563Bodipy558/5685

9、58569Bodipy564/570564570Bodipy576/589579590Bodipy581/591584592Bodipy630/650-X625642Bodipy650/665-X647665Bodipy665/676605676BodipyFl504,505511,513BodipyFLATP505514BodipyFl-Ceramide505511BodipyR6GSE528547BodipyTMR542574BodipyTMR-Xconjugate544573BodipyTMR-X,SE544570BodipyTR589617BodipyTRATP591620Bodipy

10、TR-XSE588616BO-PRO-1462481BO-PRO-3544570BrilliantSulphoflavinFF430520BTC-RatioDyeCa2+464/401533/529RatioDyeCa2+BTC-5N-atioDye,Zn2+459/417517/532RatioDye,Zn2+Calcein494517CalceinBlue373440CalciumCrimson588,589611,615CalciumGreen501,506531CalciumGreen-1Ca2+Dye506531Ca2+DyeCalciumGreen-2Ca2+506/503536C

11、a2+CalciumGreen-5NCa2+506532Ca2+CalciumGreen-C18Ca2+509530Ca2+CalciumOrange549575576CalcofluorWhite385,395,405437,440,445Carboxy-X-rhodamine(5-ROX)576601CascadeBlue377398399420423CascadeYellow399400550552Catecholamine410470CCF2(GeneBlazer)CFDA494520CFP-CyanFluorescentProtein430,433,436,(453)474,475,

12、476,(501)CyanFluorescentProteinCFP/YFPFRETChlorophyll480650ChromomycinA436-460470ChromomycinA445575CL-NERF(RatioDye,pH)504/514540RatioDye,pHCMFDA494520CoelenterazineCa2+Dye,bioluminescence(429)465Ca2+Dye,bioluminescence,nativemoleculeCoelenterazinecp(Ca2+Dye,)(430)442Ca2+Dye,bioluminescenceCoelenter

13、azinef(437)473Ca2+Dye,bioluminescenceCoelenterazinefcp452Ca2+Dye,bioluminescenceCoelenterazineh(437)464Ca2+Dye,bioluminescenceCoelenterazinehcp(433)444Ca2+Dye,bioluminescenceCoelenterazineip441Ca2+Dye,bioluminescenceCoelenterazinen(431)467Ca2+Dye,bioluminescenceCoelenterazineO460575CoumarinPhalloidi

14、n387470C-phycocyanineCPMMethylcoumarin384469MethylcoumarinCTC400-450602CTCFormazanCy2489506Cy3.18554568Cy3.5581598Cy3514552554566570Cy5.18649666Cy5.5675695Cy5649666670Cy7710,743767,805CyanGFP433(453)475(501)cyclicAMPFluorosensor(FiCRhR)500517CyQuantCellProliferationAssay480520CellProliferationAssayD

15、abcyl453Dansyl340578DansylAmine337517DansylCadaverine335518DansylChloride372518DansylDHPE336517Dansylfluoride356noneDAPI359461Dapoxyl403580Dapoxyl2374574Dapoxyl3373574DCFDA504529DCFH(DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR(Dihydorhodamine123)505534Di-4-ANEPPS496705Di-8-ANEPPS(non-rat

16、io)488498605713DiA(4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate(DCFH)505535DiD-LipophilicTracer644665LipophilicTracerDiD(DiIC18(5)644665DIDS341415Dihydorhodamine123(DHR)505535DiI(DiIC18)549,551565Dinitrophenol349DiO(DiOC18(3)484,487501,502DiR748780LipophilicTracerDiR(DiIC18(7)750779DM-NERF(

17、highpH)497/510540RatioDye,pHDNP349Dopamine340490-520DsRed558583RedfluorescentproteinDTAF494520DY-630-NHS621660HemicyanelabelforproteinsandDNADY-635-NHS634664HemicyanelabelforproteinsandDNAEBFP383447EnhancedBlueFluorescentProteinECFP436474EnhancedCyanFluorescentProteinEGFP488,498507,516EnhancedGreenF

18、luorescentProteinELF97345530Eosin524545Erythrosin529,532554,555ErythrosinITC529555EthidiumBromide510,523595,605Ethidiumhomodimer-1(EthD-1)528617Euchrysin430540EukoLightEuropium(III)chlorideEYFP513,520527,532EnhancedYellowFluorescentProteinFastBlue360440FDA494520Feulgen(Pararosaniline)570625FIF(Forma

19、ldehydInducedFluorescence)405433FITC490,494520,525FITCAntibody493517FlazoOrange375-530612Fluo-3480506,506520,527Fluo-4494516Fluorescein(FITC)490,494520,525FluoresceinDiacetate494520Fluoro-Emerald495524Fluoro-Gold(Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM1-43479598FM4-46515640FuraRed(h

20、ighpH)572657FuraRedTM/Fluo-3Fura-2,highcalcium335505ExcitationratiodyeFura-2,lowcalcium363512ExcitationratiodyeFura-2/BCECFGenacrylBrilliantRedB520590GenacrylBrilliantYellow10GF430485GenacrylPink3G470583GenacrylYellow5GF430475GeneBlazer(CCF2)GFP(S65T)498516GFPredshifted(rsGFP)498516GFPwildtype,non-U

21、Vexcitation(wtGFP)475509GFPwildtype,UVexcitation(wtGFP)395509GFPuv385508GloxalicAcid405460GranularBlue355425Haematoporphyrin530-560580Hoechst33258345487Hoechst33342347483Hoechst34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine(FluoroGold)361536Hydroxytryptamine400530Indo-1,highca

22、lcium330401EmissionratiodyeIndo-1,lowcalcium346475EmissionratiodyeIndodicarbocyanine(DiD)644665Indotricarbocyanine(DiR)748780IntrawhiteCf360430JC-1514529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS751(DNA)543712LDS751(RNA)590607LeucophorPAF370430LeucophorSF380465LeucophorWS395465Lissam

23、ineRhodamine572,577591,592LissamineRhodamineB577592LIVE/DEADKitAnimalCells494517formoredetailsreferCalcein/Ethidiumhomodimer528617toLOLO-1566580LO-PRO-1568581LuciferYellow425,428528,536,540LysoTrackerBlue373422LysoTrackerBlue-White466536LysoTrackerGreen504,534511,551LysoTrackerRed490516LysoTrackerYe

24、llow551576LysoSensorBlue374424LysoSensorGreen442505LysoSensorYellow/Blue384540MagGreen507531MagdalaRed(PhloxinB)524600Mag-FuraRed483/427659/631RatioDye,Mg2+Mag-Fura-2369/329369/330508511/491RatioDyeCa2+RatioDyeMg2+Mag-Fura-5369/330369/332505/500505/482RatioDye,Ca2+RatioDye,Mg2+Mag-Indo-1349/328349/3

25、30480/390480/417RatioDye,Ca2+RatioDye,Mg2+MagnesiumGreen506,507531MagnesiumOrange550575MalachiteGreen628MarinaBlue362459MaxiionBrilliantFlavin10GFF450495MaxiionBrilliantFlavin8GFF460495Merocyanin555578Methoxycoumarin360410MitotrackerGreenFM490516MitotrackerOrange551576MitotrackerRed578599Mitramycin4

26、50470Monobromobimane398490Monobromobimane(mBBr-GSH)398500Monochlorobimane380461MPS(MethylGreenPyronineStilbene)364395NBD466539NBDAmine450530NileRed515-555,559590,640Nitrobenzoxadidole465510-650Noradrenaline340490-520NuclearFastRed289-530580NuclearYellow365495NylosanBrilliantIavinE8G460510OregonGreen

27、503522OregonGreen488-X494517OregonGreen503522OregonGreen488490,493514,520OregonGreen500497517OregonGreen514506526PacificBlue405455Pararosaniline(Feulgen)570625PBFI340/380420ExcitationratiodyePE-Cy5488670PE-Cy7488755,767PerCP488675PerCP-Cy5.5488710PE-TexasRedRed613488613PhloxinB(MagdalaRed)524600Phor

28、witeAR360430PhorwiteBKL370430PhorwiteRev380430PhorwiteRPA375430Phosphine3R465565PhotoResist365610PhycoerythrinBPE546-565575PhycoerythrinRPE565578PKH26(Sigma)551567PKH67496520ChromaPMIA341376PontochromeBlueBlack535-553605POPO-1433457POPO-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550ProcionYellow

29、470600PropidiumIodid(PI)(305),536,538617PyMPO412,415561,564,570Pyrene360387Pyronine410540PyronineB540-590560-650PyrozalBrilliantFlavin7GF365495QSY7560QuinacrineMustard440510Red613PE-TexasRed488613Resorufin571584,585RH414532716Rhod-2552576Rhodamine550573Rhodamine110496,497520Rhodamine123507529Rhodami

30、ne5GLD470565Rhodamine6G525555RhodamineB540625RhodamineB200523-557595RhodamineBextra550605RhodamineBB540580RhodamineBG540572RhodamineGreen502527RhodaminePhallicidine558,542575,565RhodaminePhalloidine542565RhodamineRed570590RhodamineWT530555RoseBengal525,540550-600R-phycocyanineR-phycoerythrin(PE)5655

31、78rsGFP473509redshiftedGFP(S65T)S65A471504S65C479507S65L484510S65T488511SapphireGFP395511SBFI340/380420ExcitationratiodyeSerotonin365520-540SevronBrilliantRed2B520595SevronBrilliantRed4G500583SevronBrilliantRedB530590SevronOrange440530SevronYellowL430490sgBFPTM387450sgBFPTM(superglowBFP)387450Quantu

32、msSuperGloGFPAFPssgGFP474488sgGFP(superglowGFP)474509QuantumsSuperGloGFPAFPsSITS336436IonChannelsSITS(Primuline)395-425450SITS(StilbeneIsothiosulphonicAcid)365460SNAFLcalcein506/535535/620RatioDye,pHSNAFL-1508/540543/623RatioDye,pHSNAFL-2514/543546/630RatioDye,pHSNARFcalcein552/574590/629RatioDye,pH

33、SNARF1576/548635/587ExcitationandemissionratiodyeSodiumGreen506,507532Na+,K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30,509/31538/44,524/56VysisSpectrumOrange559/38,560588/48VysisSpectrumRed587,587/35612,612/51SPQ(6-methoxy-N-(3-sulfopropyl)quinolinium)344443Stilbene335440SulphorhodamineBcanC5

34、20595SulphorhodamineGExtra470570SYTO11508,510527,530DyeforDNA,RNASYTO12499,500522,519DyeforDNA,RNASYTO13488,491509,514DyeforDNA,RNASYTO14517,521549,547DyeforDNA,RNASYTO15516,518546,555DyeforDNA,RNASYTO16488,494518,525DyeforDNA,RNASYTO17621634DyeforDNASYTO18490,493507,527DyeforDNA,RNASYTO20512530Dyef

35、orDNASYTO21494517DyeforDNASYTO22515535DyeforDNASYTO23499520DyeforDNASYTO24490515DyeforDNASYTO25521556DyeforDNASYTO40420441DyeforDNASYTO41430454DyeforDNASYTO42433460DyeforDNASYTO43436467DyeforDNASYTO44446471DyeforDNASYTO45452484DyeforDNASYTO59622645DyeforDNASYTO60652678DyeforDNASYTO61628645DyeforDNAS

36、YTO62652676DyeforDNASYTO63657673DyeforDNASYTO64599619DyeforDNASYTO80531545NucleicAcidStainSYTO81530544NucleicAcidStainSYTO82541560NucleicAcidStainSYTO83543559NucleicAcidStainSYTO84567582NucleicAcidStainSYTO85567583NucleicAcidStainSYTOXBlue445470NucleicAcidStainSYTOXGreen504523NucleicAcidStainSYTOXOr

37、ange547570NucleicAcidStainTetracycline390-425525-560Tetramethylrhodamine(TRITC)555576TexasRed595620TexasRed-Xconjugate595615Thiadicarbocyanine(DiSC3)651,653674,675ThiazineRedR596615ThiazoleOrange510530Thioflavin5430550ThioflavinS430550ThioflavinTCN350460Thiolyte370-385477-488ThiozoleOrange453480TinopolCBS(CalcofluorWhite)390430TMR550573TO-PRO-1515531TO-PRO-3644657TO-PRO-5747770TOTO-1514531,533TOTO-3642660TriColor(PE-Cy5)(488)650667TRITCTetramethylRodaminelsoThioCyanate550573TrueBlue365425TruRed490695Ultralite656678UranineB420520Uvitex