Norepinephrine-Levarterenol-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemENorepinephrineCat. No.: HY-13715CAS No.: 51-41-2Synonyms: Levarterenol; L-Noradrenaline分式: CHNO分量: 169.18作靶点: Endogenous Metabolite; Adrenergic Receptor; Autophagy作通路: Metabolic Enzyme/Protease; GPCR/G Protein; NeuronalSignaling

2、; Autophagy储存式: 4C, protect from light, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight, stored under nitrogen)溶解性数据体外实验 H2O : 5 mg/mL (29.55 mM; Need ultrasonic)DMSO : 5 mg/mL (29.55 mM; ultrasonic and warming and heat to 60C)Mass Solvent1 mg 5 mg 10 mg Concentr

3、ation制备储备液1 mM 5.9109 mL 29.5543 mL 59.1086 mL5 mM 1.1822 mL 5.9109 mL 11.8217 mL10 mM 0.5911 mL 2.9554 mL 5.9109 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Norepinephrine (Levarterenol) 为 1选择性的adrenergic receptor激动剂，EC50值为 5.37 M。IC50 & Target Human Endogenous Metabolite体外

4、研究Norepinephrine (NE) bitartrate monohydrate is generally considered to be a 1-subtype selective adrenergic1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEagonist. Norepinephrine(NE) also has direct activity at the 2-adrenoceptor in higher concentrations 1.Adipocytes from the inguinal fat pad (iWA)

5、 or the interscapular fat pad (BA) are isolated from neonatal wild-type C57BL/6J mice and cultured. To examine the effect of activating AT2 upon -adrenergic signaling,cAMP production is first assessed in response to Norepinephrine (NE, 10 M) with or without CGP (10 nM)co-treatment. Norepinephrine (N

6、E) increases cAMP as expected in iWA, and CGP does not alter this effect.Norepinephrine (NE) is also known to induce lipolysis, and liberated fatty acids are required to functionallyactivate UCP1 protein and to stimulate heat production. CREB phosphorylation at Ser133 is increased afterNorepinephrin

7、e (NE) treatment and significantly attenuated with CGP co-treatment in mouse iWA 2.PROTOCOLCell Assay 2 Subcutaneous preadipocytes derived from a 38-year old non-diabetic female donor are immortalized withTERT and HPV E6/E7. For the current studies, a stable diploid clone (referred to as clone B) wi

8、th consistentdifferentiation capacity is isolated by ring cloning. Cells are grown in preadipocyte PGM2 media. Once cellsare confluent, differentiation is induced by incubation in differentiation media consisting of dexamethasone,IBMX, indomethacin, and additional insulin. Cells are differentiated f

9、or 10 days. Prior to treatment, media isreplaced with PGM2 media for one day and then switched to serum-free media overnight for treatments.Adipocytes are treated for 6 hours with vehicle, Norepinephrine (NE, 10 M), CGP (10 nM), orNorepinephrine (NE) and CGP 2.MCE has not independently confirmed the

10、 accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cardiovasc Res. 2018 Feb 1;114(2):300-311. J Am Heart Assoc. 2019 Jan 8;8(1):e009871. J Nutr Biochem. 2018 May 1;58:110-118. Neuroscience. 2019 Mar 1;401:59-72. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Aug 15;1092:220-227

11、.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. MacGregor DA, et al. Relative efficacy and potency of beta-adrenoceptor agonists for generating cAMP in human lymphocytes. Chest.1996 Jan;109(1):194-200.2. Littlejohn NK, et al. Suppression of Resting Metabolism by the Angiotensin AT2 Receptor. Cell Rep. 2016 Aug 9;16(6):1548-60.McePdfHeightCaution: Product has n