6-Thioguanine-Thioguanine-2-Amino-6-purinethiol-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE6-ThioguanineCat. No.: HY-13765CAS No.: 154-42-7Synonyms: Thioguanine；2-Amino-6-purinethiol分式: CHNS分量: 167.19作靶点: SARS-CoV; Deubiquitinase; Autophagy作通路: Anti-infection; Cell Cycle/DNA Damage; Autophagy储存式: Powder -20C 3 years4C

2、 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 10 mg/mL (59.81 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 5.9812 mL 29.9061 mL 59.8122 mL5 mM 1.1962 mL 5.9812 mL 11.9624 mL10 mM 0.5981 mL 2.9906 mL 5.9812 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存

3、式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 1.67 mg/mL (9.99 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师www.Med

4、ChemE物活性 6-Thioguanine (Thioguanine)种抗病和免疫抑制剂，为 SARS 和 MERS 冠状病毒样蛋酶 (PLpros) 的抑制剂，同时可有效抑制 USP2 的活性，IC50 值分别为 25 M 和 40 M。IC50 & Target IC50: 25 M (PLpros), 40 M (Recombinant human USP2) 3体外研究 6-Thioguanine (Thioguanine) is an anti-leukemia and immunosuppressant agent, acts as an inhibitor of SARSand

5、 MERS coronavirus papain-like proteases (PLpros) and also potently inhibits USP2 activity, with IC50s of25 M and 40 M for Plpros and recombinant human USP2, respectively 1. 6-Thioguanine (Thioguanine)affects the methylation of cytosine residues by purified DNA methyltransferases including human DNMT

6、1 andbacterial HpaII methylase. 6-Thioguanine (Thioguanine) (1 or 3 M) decreases global cytosine methylation inJurkat T cells and cytosine methylation in human cells at 3 M 2. 6-Thioguanine (Thioguanine) (18.75, 37.50,or 75.00 M) adversely affects cell viability, but with no effect on LDH or ALT act

7、ivity 3.PROTOCOLCell Assay 3 Treatments consists of 3 thiopurines (azathioprine, 6-mercaptopurine, and 6-Thioguanine (Thioguanine) ateach of 6 concentrations (0.468, 0.937, 1.875, 3.750, 7.500, and 15.000 M). Each thiopurinee is dissolvedin DMSO solution to achieve a concentration of 10 mg/mL. Steri

8、le filtered maintenance medium is used tofurther dilute each thiopurine solution to each of the 6 treatment concentrations. Twenty-four hours after thehepatocytes are plated on 96-well culture 3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研

9、献 Patent. US20180263995A1.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Chuang SJ, et al. 6-Thioguanine is a noncompetitive and slow binding inhibitor of human deubiquitinating protease USP2. Sci Rep. 2018Feb 15;8(1):3102.2. Wang H, et al. 6-Thioguanine perturbs cytosine meth

10、ylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts asa DNA demethylating agent in vivo. Biochemistry. 2009 Mar 17;48(10):2290-9.3. LaDuke KE, et al. Effects of azathioprine, 6-mercaptopurine, and 6-thioguanine on canine primary hepatocytes. Am J Vet Res. 2015Jul;76(7):649-55.McePdfHeightCaution: Product has not been fully vali