Stains-All-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEStains-AllCat. No.: HY-D0987CAS No.: 7423-31-6分式: CHBrNS分量: 559.58作靶点: Others作通路: Others储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性数据体外实验 DMSO : 65 mg/mL (116.16 mM; Need ultras

2、onic)H2O : 0.1 mg/mL (insoluble)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.7871 mL 8.9353 mL 17.8705 mL5 mM 0.3574 mL 1.7871 mL 3.5741 mL10 mM 0.1787 mL 0.8935 mL 1.7871 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Stains-All种阳离羰花青染料。体外研究Almost all of the proteins f

3、ound in skeletal muscle extracts, including the Ca2+Mg2+-ATPase and the53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin,the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-allstaining (

4、measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 forcalsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEstaining these Ca2+-binding proteins blue, Stains-a

5、ll is a more sensitive stain for these Ca2+-binding proteinsthan is Coomassie blue 1.PROTOCOLKinase Assay 1 Slab gels are fixed overnight with 25% isopropyl alcohol and washed exhaustively in 25% isopropyl alcohol toremove SDS. The gels are then stained in the dark for at least 48 h with 0.0025% Sta

6、ins-all, 25% isopropylalcohol, 7.5% formamide, and 30 mM Tris base, pH 8.8. The interaction of Stains-all with various Ca2+-binding proteins is also studied in aqueous solution. The standard solution contains 10 mM Tris base, pH 8.8,0.001% Stains-all, and 0.1% formamide. Ca2+-binding proteins (0.5 t

7、o 12 g) are added to 1.0 mL of solutionand then incubated at room temperature in the dark for 30 min. The absorbance at 600 nm is then measuredagainst a control solution, containing no protein, using a spectrophotometer 1.MCE has not independently confirmed the accuracy of these methods. They are fo

8、r reference only.REFERENCES1. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationiccarbocyanine dye Stains-all. J Biol Chem. 1983 Sep 25;258(18):11267-73.McePdfHeightCaution: Product has not been fully validated for medical applications.For