2007年10月SCIE收录的南京农业大学学术论文(非第一通讯

1、2007年10月SCIE收录的南京农业大学学术论文（非第一通讯作者单位）共12篇Record 1Author(s): Hong-Tao, W (Hong-Tao, Wang); Shao-Ling, Z (Shao-Ling, Zhang); Wang-Yi, L (Wang-Yi, Liu)Title: A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrateSource: PROGRESS IN BIOCHEMISTRY AND

2、 BIOPHYSICS, 34 (9): 991-995 SEP 2007Language: ChineseDocument Type: ArticleAuthor Keywords: ribosome-inactivating protein(RIP); double-stranded supercoiled DNA; gel electrophoresisKeywords Plus: RNA N-GLYCOSIDASE; EUKARYOTIC RIBOSOMES; CINNAMOMUM-CAMPHORA; SUPERCOILED DNA; MECHANISM; RICIN; CHAINAb

3、stract: A novel method for assaying the enzymatic activity of ribosome-inactivating proteins (RIPs) has been developed. The principle of the method is based on that RIP can remove some adenine bases from double-stranded supercoiled DNA molecules, subsequently, the deadenylated DNA was cleaved into n

4、icked and linear form. After treatment with acidic aniline, the deadenylated DNA was degraded into many small fragments, and run out of the gel. The enzymatic activities of two RIPs (trichosanthin and cinnamomin) were tested using this method, the limit of sensitivity is about 50 ng (trichosanthin)

5、and 5 ng (reduced cinnamomin). It should be emphasized that the merit of this method is to avoid the preparation of ribosome.Addresses: Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China; Henan Univ, Coll Life Sc

6、i, Kaifeng 475001, Peoples R ChinaReprint Address: Shao-Ling, Z, Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.E-mail Address: nnzsl; wyliusibs.ac.anTimes Cited: 0ISSN: 1000-3282ISI Document Delivery No.: 212MYRecord 2Author(s): Chen, LH (Chen, Lihua); Li, YH (Li, Yuho

7、ng); Li, JX (Li, Jianxu); Xu, XQ (Xu, Xueqing); Lai, R (Lai, Ren); Zou, QM (Zou, Quanming)Title: An antimicrobial peptide with antimicrobial activity against Helicobacter pyloriSource: PEPTIDES, 28 (8): 1527-1531 AUG 2007Language: EnglishDocument Type: ArticleAuthor Keywords: amphibian; antimicrobia

8、l peptide; Odorrana grahami; Helicobacter pyloriKeywords Plus: SPOTTED TORRENT FROG; SKIN SECRETIONS; IMMUNITYAbstract: An antimicrobial peptide named odorranain-HP was identified from skin secretions of the diskless odorous frog, Odorrana grahami. It is composed of 23 amino acids with an amino acid

9、 sequence of GLLRASSVWGRKYYVDLAGCAKA. By BLAST search, odorranain-HP had similarity to antimicrobial peptide odorranain-W1 but it has a different GLLR N-terminus. The cDNA encoding odorranain-HP was cloned from the cDNA library of the skin of O. grahami. This peptide showed antimicrobial activities

10、against tested microorganisms. Interestingly, odorranain-HP could exert antimicrobial capability against Helicobacter pylori, along with its antimicrobial activities similar to odorranain-W1. This is the first report of naturally occurring peptide with anti-H. pylori activity from amphibian skins. (

11、c) 2007 Elsevier Inc. All rights reserved.Addresses: Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Biotoxin Unit, Kunming 650223, Peoples R China; Nanjing Agr Univ, Life Sci Coll, Minist Agr, Key Lab Microbiol Engn Agr Environm, Nanjing, Peoples R China; Third Mil Med Un

12、iv, Dept Clin Immunol & Clin Microbiol, Chongqing 400038, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100009, Peoples R ChinaReprint Address: Lai, R, Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Biotoxin Unit, Kunming 650223, Peoples R China.E-mail Address: rla

13、i; qmzouTimes Cited: 0ISSN: 0196-9781ISI Document Delivery No.: 210UORecord 3Author(s): Xu, ZH (Xu, Zhihui); Xu, L (Xu, Liang); Lai, QY (Lai, Qiongyu); Ji, XY (Ji, Xiaoyang)Title: Microemulsion synthesis of LiFePO4/C and its electrochemical properties as cathode materials for lithium-ion cellsSource

14、: MATERIALS CHEMISTRY AND PHYSICS, 105 (1): 80-85 SEP 15 2007Language: EnglishDocument Type: ArticleAuthor Keywords: lithium iron phosphate; microemulsion synthesis; cathode material; lithium-ion cells; electrochemical performanceKeywords Plus: PULSED-LASER DEPOSITION; THIN-FILMS; PERFORMANCE; BATTE

15、RIES; IRON; COMPOSITEAbstract: The clectroactive LiFePO4/C nano-composite powders have been synthesized by a microemulsion method. The material were characterized by X-ray powder diffraction, X-ray photoelectron spectroscopic, scanning electron microscopy and its electrochemical properties were inve

16、stigated by cyclic voltammetry, AC impedance and charge-discharge tests. The as-prepared LiFePO4/C composite had a high capacity of 163 mAb g(-1) at the C/10 rate, i.e. 95.9% of the theoretical capacity. The composite also display a good rate capability and stable cycle-life. The improved electrode

17、performance originates mainly from the fine particle of nanometric dimension and the uniform size distribution in the product as well as the increase in electronic conductivity by the carbon coating. (c) 2007 Elsevier B.V. All rights reserved.Addresses: Sichuan Univ, Coll Chem, Chengdu 610064, Peopl

18、es R China; Nanjing Agr Univ, Coll Sci, Nanjing 210095, Peoples R ChinaReprint Address: Lai, QY, Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China.E-mail Address: laiqy5Times Cited: 0ISSN: 0254-0584ISI Document Delivery No.: 209VVRecord 4Author(s): Gu, XY (Gu, Xiang-Yang); Wong, JWC (Wong, Jo

19、nathan W. C.)Title: Degradation of inhibitory substances by heterotrophic microorganisms during bioleaching of heavy metals from anaerobically digested sewage sludgeSource: CHEMOSPHERE, 69 (2): 311-318 SEP 2007Language: EnglishDocument Type: ArticleAuthor Keywords: bioleaching; heavy metal; sewage s

20、ludge; Acidithiobacillus ferrooxidans; organic acidKeywords Plus: IRON-OXIDIZING BACTERIA; WASTE-WATER SLUDGE; THIOBACILLUS-FERROOXIDANS; ACIDOPHILIC BACTERIA; ORGANIC-COMPOUNDS; REMOVAL; GROWTHAbstract: The presence of organic acids was found to be inhibitory to the bioleaching of sewage sludge and

21、 the objective of the present study was to elucidate the roles of heterotrophic microorganisms in removing organic acids during the bioleaching of heavy metals from anaerobically digested sewage sludge. Microbiological analysis showed that acetic and propionic acids posed a severe inhibitory effect

22、on iron-oxidizing bacteria as reflected by a sharp decrease in their viable counts in the first 4 d and it only started to increase 2 d after the depletion of both acids. Biodegradation of these inhibitory organic acids was revealed by sharp increases in total fungi and acidophiles between day 3 and

23、 day 5 which coincided with degradation of organic acids. This was further confirmed by the increases in total counts of both acetate and propionate degraders in the same period. Two yeast strains Y4 and Y5 with strong ability to degrade acetate and/or propionate were isolated and identified as Pich

24、ia sp. and Blastoschizomycetes capitatus, respectively. R capitatus Y5 was an more important player in removing the inhibitory organic acids during the bioleaching process since it could utilize both acetate and propionate as sole carbon source while Pichia sp. Y4 was an strict acetate degrader. Res

25、ults from the present study not only provided the evidence for biodegradation of organic acids by heterotrophs, but also disclosed a biological mechanism for the initiation of bioleaching of organic acid-laden sewage sludge. (c) 2007 Elsevier Ltd. All rights reserved.Addresses: Hong Kong Baptist Uni

26、v, Dept Biol, Kowloon, Hong Kong, Peoples R China; Nanjing Agr Univ, Dept Microbiol, Nanjing 210095, Peoples R ChinaReprint Address: Wong, JWC, Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China.E-mail Address: jwcwong.hkTimes Cited: 0ISSN: 0045-6535ISI Document Delivery No.: 210

27、QURecord 5Author(s): Wang, SG (Wang, Shu-Guang); Gong, WX (Gong, Wen-Xin); Liu, XW (Liu, Xian-Wei); Tian, L (Tian, Lin); Yue, QY (Yue, Qin-Yan); Gao, BY (Gao, Bao-Yu)Title: Production of a novel bioflocculant by culture of Klebsiella mobilis using dairy wastewaterSource: BIOCHEMICAL ENGINEERING JOUR

28、NAL, 36 (2): 81-86 SEP 15 2007Language: EnglishDocument Type: ArticleAuthor Keywords: bioflocculant; dairy wastewater; Klebsiella mobilis; substrateKeywords Plus: CORYNEBACTERIUM-GLUTAMICUM; BIOPOLYMER FLOCCULANT; CITROBACTER SP; REMOVAL; ACIDS; SUSPENSION; REA-11Abstract: In this study, dairy waste

29、water, with 2% added ethanol (v/v), was used as a substrate for the production of a novel bioflocculant by Klebsiella mobilis. Using optimized conditions for the production of the novel bioflocculant, 2.58 g of crude bioflocculant per liter broth was produced with a flocculating activity of 95.4%. C

30、hemical analysis showed that the novel bioflocculant was a polysaccharide where no amino acids were detected. The bioflocculant possesses flocculating activity comparable or superior to that of bioflocculant produced using conventional substrate. The bioflocculant was effective in flocculating some

31、disperse dyes in aqueous solutions, in particular, disperse Violet HFRL with a decolorization efficiency of 91%. (c) 2007 Elsevier B.V. All rights reserved.Addresses: Shandong Univ, Sch Engn & Environm Sci, Jinan 250100, Peoples R China; Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peo

32、ples R ChinaReprint Address: Wang, SG, Shandong Univ, Sch Engn & Environm Sci, Jinan 250100, Peoples R China.E-mail Address: wsgTimes Cited: 0ISSN: 1369-703XISI Document Delivery No.: 213MGRecord 6Author(s): Li, ZG (Li, Zi-gang); Bian, CZ (Bian, Chuan-zhou); Jie, XL (Jie, Xiao-lei)Title: Characteris

33、tic of Cd sorption in the copper tailings wasteland soil by amended dissolved organic matter from fresh manure and manure compostSource: AFRICAN JOURNAL OF BIOTECHNOLOGY, 6 (3): 227-234 FEB 5 2007Language: EnglishDocument Type: ArticleAuthor Keywords: Cd sorption; copper tailings wasteland soil; fre

34、sh manure; manure compostKeywords Plus: BINDING ABILITY; METAL-BINDING; HUMIC-ACID; SLUDGE; WATER; FRACTIONATION; COMPLEXATION; SUBSTANCES; ADSORPTION; EXTRACTSAbstract: The aim of this study was to investigate the effects of dissolved organic matter (DOM) from fresh manure and its compost on the so

35、rption of Cd on copper tailings wasteland and normal farmland soils, in Tongling City, China. In the presence of DOM, Cd sorption capacity decreased markedly for both soils, especially for the copper tailings wasteland. The Cd sorption isotherms could be well described by the Freundlich equation (r(

36、2) = 0.98), and the binding intensity parameter of soils in the presence of fresh manure DOM was lower than compost DOM. An increase in DOM concentration significantly reduced the sorption of Cd by both soils. Within the Cd and DOM concentration range studied, the decrease in Cd sorption caused by f

37、resh manure DOM was consistently greater than that of compost DOM. Moreover, the reduction of Cd sorption caused by DOM was more obvious in the soil with higher pH. In addition, the sorption of Cd increased with an increase in pH for both soils without the addition of DOM, while Cd sorption in the p

38、resence of DOM was unexpectedly decreased with an increase in pH at a pH 6.6.Addresses: Zhengzhou Colol Anim Husb Engn, Zhengzhou 450003, Peoples R China; Nanjing Agr Univ, Nanjing 210095, Peoples R ChinaReprint Address: Li, ZG, Zhengzhou Colol Anim Husb Engn, Zhengzhou 450003, Peoples R China.E-mai

39、l Address: Times Cited: 0ISSN: 1684-5315ISI Document Delivery No.: 199CJRecord7Author(s): Xiong, AS (Xiong, Ai-Sheng); Peng, RH (Peng, Ri-He); Cheng, ZM (Cheng, Zong-Ming); Li, Y (Li, Yi); Liu, JG (Liu, Jin-Ge); Zhuang, J (Zhuang, Jing); Gao, F (Gao, Feng); Xu, F (Xu, Fang); Qiao, YS (Qiao, Yu-Shan)

40、; Zhang, Z (Zhang, Zhen); Chen, JM (Chen, Jian-Min); Yao, QH (Yao, Quan-Hong)Title: Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostabilitySource: PROTEIN ENGINEERING DESIGN & SELECTION, 20 (7): 319-325 JUL 2007Language: EnglishDocument Type: ArticleAuthor Keywords:

41、 beta-glucuronidase; directed evolution; enzyme properties; structure-function analysis; thermostabilityKeywords Plus: DIRECTED EVOLUTION; IN-VITRO; SCREENING STRATEGIES; THERMAL-STABILITY; RAPID EVOLUTION; PROTEIN; GENE; MUTAGENESIS; VACCINES; MARKERAbstract: To achieve a thermostable beta-glucuron

42、idase (GUS) and identify key mutation sites, we applied in vitro directed evolution strategy through DNA shuffling and obtained a highly thermostable mutant GUS gene, gus-tr, after four rounds of DNA shuffling and screening. This variant had mutations in 15 nucleic acid sites, resulting in changes i

43、n 12 amino acids (AAs). Using gus-tr as the template, we further performed site-directed mutagenesis to reverse the individual mutation to the wild-type protein. We found that six sites (Q493R, T509A, M532T, N550S, G559S and N566S) present in GUS-TR3337, were the key AAs needed to confer its high th

44、ermostability. Of these, Q493R and T509A were not reported previously as important residues for thermostability of GUS. Furthermore, all of these six mutations must be present concurrently to confer the high thermostability. We expressed the gus-tr3337 gene and purified the GUS-TR3337 protein that c

45、ontained the six AA mutations. Compared with the wild-type protein which lost its activity completely after 10 min at 70 degrees C, the mutant GUS-TR3337 protein retained 75% of its activity when heated at 80 degrees C for 10 min. The GUS-TR3337 exhibited high activity even heated at 100 degrees C f

46、or 30 min on nitrocellulose filter. The comparison of molecular models of the mutated and wild-type enzyme revealed the relation of protein function and these structural modifications.Addresses: Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China; Univ Tennessee, Dept Plant

47、Sci, Knoxville, TN 37996 USA; Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA; Nanjing Agr Univ, Coll Horticulture, Nanjing 210095, Peoples R China; Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R ChinaReprint Address: Yao, QH, Shanghai Acad Agr Sci, Biotechnol Res Inst, 2

48、901 Beidi Rd, Shanghai 201106, Peoples R China.E-mail Address: yaoquanhongcnTimes Cited: 0ISSN: 1741-0126ISI Document Delivery No.: 209NQRecord 8Author(s): Zhang, CQ (Zhang, C. Q.); Yuan, SK (Yuan, S. K.); Sun, HY (Sun, H. Y.); Qi, ZQ (Qi, Z. Q.); Zhou, MG (Zhou, M. G.); Zhu, GN (Zhu, G. N.)Title: S

49、ensitivity of Botrytis cinerea from vegetable greenhouses to boscalidSource: PLANT PATHOLOGY, 56 (4): 646-653 AUG 2007Language: EnglishDocument Type: ArticleAuthor Keywords: Botryotinia fuckeliana; fungicide resistance; grey mould; kresoxim-methyl; pyrimethanilKeywords Plus: BOTRYOTINIA-FUCKELIANA;

50、FUNGICIDE RESISTANCE; PROTECTED CROPS; DICARBOXIMIDE; ANILINOPYRIMIDINE; BIOSYNTHESIS; VINEYARDS; MIXTURES; STRAINSAbstract: Between 2004 and 2006, 228 isolates of Botrytis cinerea from two regions in China were characterized for baseline sensitivity to boscalid, a new active ingredient that interfe

51、res with succinate ubiquinone reductase in the electron transport chain. The isolates showed similar sensitivity in different years and regions. Baseline sensitivities were distributed as unimodal curves with mean EC, values of 1-07 ( +/- 0 center dot 11) and 0 center dot 42 ( 0.05) mg L-1 for inhib

52、ition of mycelial growth and conidial germination, respectively. Laboratory studies were conducted to evaluate the risk of development of resistance to boscalid. Boscalid-resistant mutants were obtained by UV-treatment at lower frequencies and with smaller resistance factors than pyrimethanil-resist

53、ant mutants. All boscalid-resistant mutants were also significantly more sensitive to Qo inhibitors than their wild-type parents and showed reduced sporulation in vitro and pathogenicity on aubergine leaves. The results suggested that the risk of resistance developing for boscalid was lower than for

54、 pyrimethanil. However, as B. cinerea is a high-risk pathogen, appropriate precautions against resistance development should be taken. Synergism between the activity of boscalid and that of kresoxim-methyl was observed.Addresses: Zhejiang Univ, Coll Agr & Biotechnol, Inst Pesticide & Environm Toxico

55、l, Hangzhou 310029, Peoples R China; Minist Agr, Inst Control Agrochem, Ctr Agrochem Biol & Environm Technol, Beijing 100026, Peoples R China; Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R ChinaReprint Address: Zhang, CQ, Zhejiang Univ, Coll Agr & Biotechnol, Inst Pesticide & Envir

56、onm Toxicol, Hangzhou 310029, Peoples R China.E-mail Address: cqzhang9603Times Cited: 0ISSN: 0032-0862ISI Document Delivery No.: 196LURecord 9Author(s): Zhang, YL (Zhang, Y. L.); Qin, BQ (Qin, B. Q.); Liu, ML (Liu, M. L.)Title: Temporal-spatial variations of chlorophyll a and primary production in M

57、eiliang Bay, Lake Taihu, China from 1995 to 2003Source: JOURNAL OF PLANKTON RESEARCH, 29 (8): 707-719 AUG 2007Language: EnglishDocument Type: ArticleKeywords Plus: LARGE SHALLOW LAKE; SATELLITE CHLOROPHYLL; PHYTOPLANKTON; OCEAN; SEA; BIOMASS; WATERS; LIGHT; VARIABILITYAbstract: There are few long-te

58、rm data sets on primary production in a lake, which can be used to validate the output from a production model To address this need, we determined the temporal- spatial variations of chlorophyll a (Chl a) and primary production (PPeu) based on the vertically generalized production model (VGPM) by us

59、ing 742 samplings at seven sites in Meiliang Bay in Lake Taihu from 1995 to 2003. An empirical model estimating primary production (PPin) was used to validate VGPM PP., and the dominant factors controlling PP were determined. Markedly higher Chl a and PPeu values wen, recorded in Meiliang Bay in 199

60、6 and 1997 than in other years and a marked decrease in Chl a and PPeu was found between 2001 and 2003. Peaks of Chl a typically appeared in summer (June-August) and minima occurred in winter (January). The highest daily mean PPeu usually occurred in summer (June); the exception was at site 1, where