Cordycepin-3-Deoxyadenosine-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECordycepinCat. No.: HY-N0262CAS No.: 73-03-0Synonyms: 3-Deoxyadenosine分式: CHNO分量: 251.24作靶点: MMP; Autophagy作通路: Metabolic Enzyme/Protease; Autophagy储存式: Powder -20C 3 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 50

2、mg/mL (199.01 mM; Need ultrasonic)H2O : 4 mg/mL (15.92 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.9803 mL 19.9013 mL 39.8026 mL5 mM 0.7961 mL 3.9803 mL 7.9605 mL10 mM 0.3980 mL 1.9901 mL 3.9803 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请

3、先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (9.95 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (9.95 mM); Clear solution3. 请

4、依序添加每种溶剂： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 2.5 mg/mL (9.95 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Cordycepin从冬夏草中分离的种核苷衍物，在类风湿性关节炎滑膜成纤维细胞 (RASF) 中，抑制 IL-1 诱导的 MMP-1 和 MMP-3 表达，这种作存在剂量依赖性。IC50 & Target MMP-1 MMP-3体外研究 Cordycepin is a potent inhibito

5、r of IL-1-induced chemokine production and MMP expression and stronglyblocks the p38/JNK/AP-1 signalling pathway in RASFs. The effect of Cordycepin on cellular toxicity of RASFsis assessed using MTT assay. Treatment of RASFs with Cordycepin (50 M or 100 M) for 24 h does notcause any significant chan

6、ge in cell viability. However, cell viability is slightly decreased when cells areincubated with 100 M Cordycepin for 48 h 1.PROTOCOLCell Assay 1 RASFs (2104 cells/well) are treated with various concentrations of Cordycepin (50 M or 100 M). Afterincubation for 1 h, 12 h and 24 h, cells are washed tw

7、ice with PBS, MTT (0.5 mg/mL PBS) is added to eachwell and incubated at 37C for 30 min. Formazan crystals formed are dissolved by adding DMSO (100 L/well) and the absorbance is read at 570 nm using a microplate reader 1.MCE has not independently confirmed the accuracy of these methods. They are for

8、reference only.户使本产品发表的科研献 Nat Commun. 2019 Jun 10;10(1):25See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Noh EM, et al. Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts.Rheumatology (Oxford). 2009 Jan;48(1):45-8.McePdfHeightCaution: Product has not been fully validated for medical applications