咪唑立宾作用机制 - Medchemexpress - MCE中国

1、Product Data SheetMizoribineCat. No.: HY-17470CAS No.: 50924-49-7分式: CHNO分量: 259.22作靶点: HCV; SARS-CoV作通路: Anti-infection储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 35 mg/mL (135.02 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcent

2、ration制备储备液1 mM 3.8577 mL 19.2886 mL 38.5773 mL5 mM 0.7715 mL 3.8577 mL 7.7155 mL10 mM 0.3858 mL 1.9289 mL 3.8577 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储

3、备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (9.64 mM); Clear solution此案可获得 2.5 mg/mL (9.64 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DM

4、SO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (9.64 mM); Clear solutionPage 1 of 2 www.MedChemE此案可获得 2.5 mg/mL (9.64 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 20% 的 SBE-CD 理

5、盐溶液中，混合均匀。3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (9.64 mM); Clear solution此案可获得 2.5 mg/mL (9.64 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Mizoribine (NSC 289637) 抑制 HCV RNA 复制，具有抗 HCV 活性，IC50 约为 100 M。Mizori

6、bine种免疫抑制剂1。Mizoribine 抑制 IMPDH。Mizoribine 抑制 SARS-CoV 复制，对 SARS-CoV Frankfurt-1 和 SARS-CoV HKU39849 的IC50 分别为 3.5 g/mL 和 16 g/mL2。IC & Target HCV1; IMPDH; SARS-CoV2体外研究 Mizoribine, isolated from culture medium of the mold Eupenicillium brefeldianum M-2166, acts as animmunosuppressant which exerts i

7、ts effects without severe side effects1.Mizoribine is an inosine-5-monophosphate dehydrogenase (IMPDH) inhibitorused for renal transplantation,autoimmune diseases and steroid-resistant nephrotic syndrome 2.体内研究 Mizoribine suppresses collagen-induced arthritis. Mizoribine (10, 20 and 50 mg/kg; Admini

8、stered orally 5 days a weekfor 12 weeks) reduces the arthritis score in a dose-dependent fashion, showing significant suppression, even at a doseof 10 mg/kg3.Animal Model: Male DBA/1 J mice with collagen-induced arthritis3Dosage: 10, 20 and 50 mg/kgAdministration: Administered orally 5 days a week f

9、or 12 weeksResult: Showed dose-dependency of the suppressive effect.户使本产品发表的科研献 Molecules. 2019 May 3;24(9). pii: E1723.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Naka K, et al. Mizoribine inhibits hepatitis C virus RNA replication: effect of combination with in

10、terferon-alpha. Biochem Biophys Res Commun. 2005 May13;330(3):871-9.2. Saijo M, et al. Inhibitory effect of Mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus.Antiviral Res. 2005 Jun;66(2-3):159-63.Page 2 of 3 www.MedChemE3. Kamada H, et al. Effect of Mizoribine on collagen-induced arthritis in mice. Jpn J Pharmacol. 1996 Feb;70(2):169-75.McePdfHeightCaution: Product has not been fully validated for medic