WATERS公司的UPLC-TQD培训资料MS6调谐页面ppt课件

1、 设定离子模式Ion mode is set 设定仪器参数Instrument parameters are set. API 和 碰撞气Collision gases ）已经调节完毕. 先导化合物Parent compounds已经确定并优化 子离子Daughter ions已经决定并优化 设置Mass 分辨率Resolution ） 碰撞能量Collision Energies已优化 执行采集 仪器调谐页面(MS Tune)使用仪器快捷菜单InstrumentShortcut Item）Use the New, Open, Save or Print tools or use the Fi

2、le menu for these operations.OperateStandbyPurge Refill SyringeStart/Stop 显示质量数(Da)的范围或者宽度显示的中心质量数显示的信号增益MS Scan等功能的显示在底部或者顶部的工具栏双击鼠标，可以增加或者减少增益的设置点击 X 一下，可以恢复前面的范围和增益设置，再点击一下可以回到缺省的设置。点击选择是否显示Set = Parent/ precursor IonSet是母离子/先导离子16 data points/amu8 data points/amu4 data points/amu100 101 102 103

3、104100 %94 %97 %Best ResolutionSmooth AppearanceCompromisem/zMM+1100-500 amu - Continuum - 8 pts/amu343344345346347348349350351352353354355356357358359360361362363364365366367368369370m/z0100%0100%0100%Test3 9 (0.190)Scan ES- 3.04e6353.22354.23Test2 9 (0.190)Scan ES- 3.21e6353.23354.33Test1 9 (0.196

4、)Scan ES- 3.65e6353.19354.23355.0916 Data Points / DaltonNo Smoothing8 Data Points / DaltonNo Smoothing4 Data Points / DaltonNo SmoothingProstaglandin PGF2a - ESI negativeAcquisition Mode : ContinuumMass range : 100-500 amuScan Speed : 400 amu/sBASELINE LEVEL=00Intensitym/z BASELINE LEVEL=10Intensit

5、yBASELINE LEVEL=50IntensityIon Current XIon Current Z Ion Current YIon Current Y Ion Current X Baseline Level typically set to 00100020000500100015000500100015000200400600 Set to 0 (Ion Counting Threshold Disabled) Set to 2 (2 time the RSD of noise observed without ion beam) Set to 4 (4 times the RSD of noise observed without ion beam) Set to 10 (10 times the RSD of noise observed without ion beam)Higher IntensityLower IntensityBest Adjust