GOODx27s缓冲液

1、BufferReferenceCenterUsefulpHRangesofSelectedBiologicalBuffers(25C,0.1M)BuffersUsefulpHRangepKa(at20)pKa(at25) pKa(at37)ReagentGradeBPCGradeUltraGradeMES5.5-6.76.166.105.97M8250M2933M5287Bis-Tris5.872n/a6.506.36B9754B4429B7535ADA6.0726.656.596.46A9883n/aA8074aces6.1756.886.786.54A9758A3594A7949PIPES

2、6.1156.806.766.66P6757P1851P8203MOPSO6.276n/a6.906.75M8389n/an/aBis-TrisPropane6.3-9.5n/a6.8,9.0n/aB6755B4679B9410BES6.4787.177.096.90B9879B4554B6420MOPS6.5797.287.207.02M1254M3183M5162TES6.8-8.27.507.407.16T1375T5691T6541HEPES6.8-8.27.557.487.31H3375H4034H7273DIPSO7.0-8.2n/a7.607.35D9648n/aD0306MOB

3、S6.9-8.3n/a7.60n/aM3295n/an/aTAPSO7.0-8.2n/a7.607.39T9269T5566T0432Trizma7.0-9.08.208.067.72T1503T6066T6791HEPPSO7.1-8.5n/a7.806.66H3137n/an/aPOPSO7.2-8.5n/a7.807.63P3405n/aP7088TEA7.3-8.3n/a7.80n/aT1377n/an/aEPPS7.3-8.7n/a8.00n/aE9502E0276E1894Tricine7.4-8.88.168.057.80T0377T5816T9784Gly-Gly7.5-8.9

4、n/a8.20n/aG1002G3915G7278Bicine7.6-9.08.358.268.04B3876n/aB8660HEPBS7.6-9.0n/a8.30n/aH6903n/an/aTAPS7.7-9.18.498.408.18T5130T5316T9659AMPD7.8-9.7n/a8.80n/aA9754n/aA9074TABS8.2-9.6n/a8.90n/aT1302n/an/aAMPSO8.3-9.7n/a9.009.10A6659n/aA7585CHES8.6-10.09.559.499.36C2885n/aC8210CAPSO8.9-10.3n/a9.609.43C22

5、78n/aC8085AMP9.0-10.5n/a9.70n/aA9879n/aA9199CAPS9.7-11.110.5610.4010.02C2632n/aC6070CABS10.0-11.4n/a10.70n/aC5580n/an/aTrizmaBufferTablepHvs.Temperature（三羟甲基氨基甲烷）pHatTemperatureg/Lfor0.05MSolution5C25C37CTrizmaHCLTrizmaBase7.767.206.917.020.677.897.307.026.850.807.977.407.126.610.978.077.507.226.351

6、.188.187.607.306.061.398.267.707.405.721.668.377.807.525.321.978.487.907.624.882.308.588.007.714.442.658.688.107.804.022.978.788.207.913.543.348.888.308.013.073.708.988.408.102.644.039.098.508.222.214.369.188.608.311.834.659.288.708.421.504.909.368.808.511.235.139.478.908.620.965.329.569.008.700.765

7、.47PotassiumPhosphateMonobasicAnhydrousg/LSodiumPhosphateDibasicHeptahydrateg/L23CpHPotassiumPhosphateMonobasicAnhydrousg/LSodiumPhosphateDibasicHeptahydrateg/L23CpH22.43.495.710.8029.516.922.084.295.89.3632.737.021.605.375.97.9235.957.121.056.606.06.7238.637.220.408.056.15.5241.317.319.569.936.24.5

8、643.467.418.6012.076.33.8445.077.517.6414.226.43.1246.687.616.4416.906.52.5248.557.715.0020.126.62.0449.097.813.5623.346.71.6849.897.912.2426.296.81.2750.818.0Citricacidmonohydrate,C6H8O7?H2O,M.wt.210.14;0.1M-solutioncontains21.01g/l.Na2HPO4,M.wt.141.98;0.2M-solutioncontains28.40g/l,orNa2HPO4?2H2O,M

9、.wt.178.05;0.2M-solutioncontains35.61g/l.xml0.1M-citricacidandyml0.2M-Na2HPO4mixed.PHxml0.1M-citricacidyml0.2-Na2HPO42.689.1010.902.884.1515.853.079.4520.553.275.3024.703.471.5028.503.667.8032.203.864.5035.504.061.4538.554.258.6041.404.455.9044.104.653.2546.754.850.7049.305.048.5051.505.246.4053.605

10、.444.2555.755.642.0058.005.839.5560.456.036.8563.156.233.9066.106.430.7569.256.627.2572.756.822.7577.257.017.6582.357.213.0586.957.49.1590.857.66.3593.65Citricacidmonohydrate,C6H8O7?H2O,M.wt.210.14;0.1M-solutioncontains21.01g/l.Trisodiumcitratedihydrate,C6H5O7Na3?2H2O,M.wt.294.12;0.1M-solutioncontai

11、ns29.41g/l.xml0.1M-citricacidandyml0.1M-trisodiumcitratemixed.pHxml0.1M-citricacidyml0.1M-trisodiumcitrate3.082.018.03.277.522.53.473.027.03.668.531.53.863.536.54.059.041.04.254.046.04.449.550.54.644.555.54.840.060.05.035.065.05.230.569.55.425.574.55.621.079.05.816.084.06.011.588.56.28.092.0Sodiumac

12、etatetrihydrate,CH3COONa?3H2O,M.wt.136.09;0.2Msolutioncontains27.22g/l.xml0.2M-NaOAcandyml0.2M-HOAcmixed.pH,18Cxml0.2M-NaOAcyml0.2M-HOAc3.710.090.03.812.088.04.018.082.04.226.573.54.437.063.04.649.051.04.859.041.05.070.030.05.279.021.05.486.014.05.691.09.0Na2HPO4?2H2O,M.wt.178.05;0.2M-solutioncontai

13、ns35.61g/l.Na2HPO4?12H2O,M.wt.358.22;0.2M-solutioncontains71.64g/l.NaH2PO4?H2O,M.wt.138.01;0.2M-solutioncontains27.6g/l.NaH2PO4?2H2O,M.wt.156.03;0.2M-solutioncontains31.21g/l.xml0.2M-Na2HPO4,yml0.2M-NaH2PO4;dilutedto100mlwithH2O.pH,25Cxml0.2M-Na2HPO4yml0.2M-NaH2PO45.84.046.06.06.1543.856.29.2540.756

14、.413.2536.756.618.7531.256.824.525.57.030.519.57.236.014.07.440.59.57.643.56.57.845.754.258.047.352.65Imidazole,C3H4N2,M.wt.68.08.25ml0.2M-imidazole(13.62g/l),xml0.2M-HCl,dilutedto100mlwithH2O.10.81Na2CO3?10H2O,M.wt.286.2;0.1M-solutioncontains28.62g/l.NaHCO3,M.wt.84.0;0.1M-solutioncontains8.40g/l.xml0.1M-Na2CO3andyml0.1M-Na2HCO3mixed.PHxml0.1M-Na2CO3yml0.1M-20C37CNaHCO39.28.810909.49.120809.59.430709.89.540609.99.7505010.19.9604010.310.1703010.510.3802010.810.69010HelpfulFormulasPercentagebyweight(w/v)(%bufferdesired/100)xfinalbuffervolume(ml)=gofstartingmaterialneeded.MolarSoluti