生理心理学课件第5章研究方法与策略

1、26Sarbon paste 碳糊6Aspiration lesions吸引器破壤法利用吸引器破壤大解表用的组缴.Copyright (C) Allyn & Bacon 2004Radio-frequency lesion 建破塌法利用甯插通人强力甯康座生高炖幼毁特定组缴 1可虑理敕深图的组.班中 Rad io frequency lesion.大鼠内侧视前区射频毁损9Copyright (C) Allyn & Bacon 2004A ExcitotOlic Mon,兴奋毒性毁损尸 ”'-.J J ,一；.：.；马人一i .mi向11一C11©QMbyNWBM

2、KiCopyright (C) Allyn & Bacon 2004Knife cuts切除法一用以物除特定的速结or tract少占fcl 7Mi 闻骂 fa*mmmk13Copyright (C) Allyn & Bacon 2004雄产 M -an mSR吗 蚓H僧BO wfl酶5W hart * of kiwn (FlgmwS.SI使用立体定位仪进行实验0 0 mmcl lesion iFmijra 5.5；-7.0 mm+0.6 nnOOnn19faiget al lesion 卜妻 (Figure 5.30)(C) Allyn & Bacon 2004Ste

3、reotaxic surgery立避定位手街Stereotaxic instrument 立骰 定位俵,Stereotaxic atlas立般定位圈方卷copyrighted) An &feacOn 2004211)IT；.Copyright (C) Allyn & Bacon 20040 力.MHlLlLULlJjtFigure G.12 A 助力*削31,比 irislrn- r”9小 a：iEisheci 5 l味m Imad. Ihe W门 ha. opened, 5prflad. and 3 cif- Clfi n 业dll removpij n 2口W，nscM；Q

4、门cil iih?c;rodos he：fj fjih iiifun diHercrit Eur*,、l口*if Hmcirzna AHrl "lil fVia TlJ- ora somewhat from the 0/ fthown in pjoviau-s figures. (Adaled hewn lk- I,咫71)i In载玻片投入化 学试剂中染色10%的福尔马 林灌流脑组织动物麻醉，用生 理盐水灌流心脏切片机，logo 微米的薄片33 . Copyright (C) Allyn & Bacon 2004图11-41大脑神经元本冲过'8Miss臼丰密而粗J

5、.工涌现34Copyright (C) Allyn & Bacon 20040 6-2 运动神经元；再&前陶.U曰用染色，陲的1 .原家牲星彤歌茂细踞4之料经承纤维；3少突映质细BQ; 4宗院性墨形株质细18： 5运动神籍元；母制黑；工小胶用细胞；包少突35Copyright （C） Allyn & Bacon 2004114小股质 Microglia （瘫大脑）镀银高倍：胞体呈长鞠圆形或梭形,从胞体长轴两端伸出树枝状突起（.）,36Copyright (C) Allyn & Bacon 2004图112原浆性星形胶质细胞PrutopiuxniKi镀银忠港：翎鲍

6、:突清:亚而忸-37Copyright (C) Allyn & Bacon 2004n ii3纤维性星形胶质细as Fibrus astixjc> lc （兔大迪） 版银 高卷.细胞有许多细而长呈放射状的突起（一八 部分con 200438'Copyright (C) Allyn & Ba115少突胶质 jd rccijtc f兔大脑）高停士胞体13形或卵圆后.有35条的突起）.短39Copyright (C) Allyn & Bacon 200422 Once we know that a p窗Hieul3r brain region ia Involv

7、ed in a particular function, we may askwhat structures pro vid 白 inputs to the region and what structures receive outputs from it.3745Copyright (C) Allyn & Bacon 2004AnterogradeTracingPHA-L is m|Kted into 立旭gon of the brain Md 场kgn up by cfendnles and Ml bodiesAxons. and termirial bultohs-can be

8、 seen undtr Ch» microKop« 'PHA-L is 和即ispomd by axdpla3mle How 轴浆.1用PHA-L追踪传出轴突的基本原理 An anterograde labeling method.i ?H>4 西¥ Allfn 工日ym顺行标记法，PHA-L被注入VMH中，被树突摄取通过细胞轴突传至终扣，在 PAG发现了标记的轴突和终扣47Copyright (C) Allyn & Bacon 2004* A r或rogrwde tracing method.过轴突逆向传回胞体，照片显示胞体位于杏仁内侧核(

9、杏仁内侧核是 VM H传入轴突的部pyr密CWn & Bacon 2004的/-s；riicl)uros,伯 Th«n sw axons and tenninals in PAGin冏：PHA-L in刖tn>g用S tracing: m)ect fluorogotd In VMH2t Th#n c«lt bod m madliai amygdala7 behavior顺行标记唠逆行 标记法显示的VM的传 入及传出通路之一Copyright (C) Allyn & Bacon 2004A Localization of a peptide by mea

10、ns of imrTiiunocytOiChemiistry.大鼠前脑 冠状切面 的一部分, 金色（外侧 隔区）和铁 锈色（第三 脑室）纤维 是含有抗 利尿素的 轴突和末 梢 Ooublo labclirig. u»ing immunceytoahoiriistry untf antorcgrado tracing双标记<b Kd E>r 4时H i Secm53Copyright (C) Allyn & Bacon 2004Explanation of the Use of In Situ Hybridization 5一、，讣6-“用来检测玻片标本中染色体上的

11、牛I殊DNA序列。最初是使用带放射性的DNA探针，通过放射自显影来显示位置。后来又发明了免疫探针法，将探针核甘酸的侧链加以改造，探针杂交后，侧链可被带有荧光标记Aadioadive RNA complementary toRNA ol inlsrest的抗体所识别，从而显示出位置。bound 10 messenger ANAMessenger RNA56Copyright (C) Allyn & Bacon 2004a In Situ Hybridization 原位杂交Copyright (C) Allyn & Bacon 2004Mua；加的¥4mvg UMtrA

12、iW 5如统而呵7CapyiiitlM 针？Q11 by 四 lyn&Barm， fiTMS2003 年1060Copyright (C) Allyn & Bacon 2004 Transcrental magnetic stimulation. (TMS )61Copyright (C) Allyn & Bacon 20043159神经系统活化=>MRI信号增加人颅脑的正中矢状位MRI成像（判断单词是否押韵时） A Function曰I MRI scan of a human brain.woman)04 PET scans of a human brain (

13、horizontal sections).A PET scans showing uplake of radioactive L-DOPA in the ba&al ganglia of a patient with parkinsonian symptoms induced by a toxic chemical before and after receiving a transplant of fetal dopaminergic neurons.口不胎 手术前(b)术后13个月74Copyright (C) Allyn & Bacon 2004NormalParkins

14、onPrePost10日 6 d 2 (EE) UQ_lnO9H 一旦-Eds1g 1CTZ 0111010012104Temporal Resolution (sec):msecCopyright (C) Allyn & Bacon 2004闾 EKChtdRtlaxed. «v» clewed叫他卿叫州硼肺硼削仍W确© OrMH昏昏欲睡3gB'Ng加CMN1rM(D) AslMp2vZ咻小、AIE Daep sloop87Copyright (C) Al89Electrodes attached to the scalp of"ear

15、th subject are connected t。，.Electrodes in geodesic sensor net 电极感应网Copyright (C) Allyn & Bac触了卬1.明 Autoradiographs of cross sections through the brains of rats that had been injected with carbon 14-labeled 2-deoxyglucose duringi ttie day (lefti and the night (right).S3 2004 叫 Allyn A Bacon4950M

16、icrodialysisProbe DetailsDentalplasticSubstances in extracellularFluid is pumped through inner canntilaFluid iscaHected and analyzedDilyas tubing、66A A <Ikjid drffuso through t%&硒 &博 tubing101Copyright (C) Allyn & Bacon 2004Cerebral dialysis大月街透析法-可浏量躺中特定叵域,於特定畤冏叫的林藤博选物w的牲放量，但科烦度校差1 r

17、esolution time =5 * 10 min.102Copyright (C) Allyn & Bacon 20044.19 A Neural Pathway Implicated in Drug Abuse (Part 1 jDialvsis mmbrne Ikipimine 103Copyright (C) Allyn & Bacon 2004* HniMUtm. «rr 即 ofmicFOpip«ltes r iaritoptiore-sisEng micmelactrodG106Copyright (C) All5556774.13 Pw Oistribution of 0 pi ale Receptors in the Rat Brain黄色区域是受体所在位置OtfnctEy bWh119MuJmI ttulJm 丘脑内侧核Hippocampus尾状核Caudflh'Inferior平RiO!皿导水管周围灰质大大鼠脑部放B自显影术Copyright (C) Allyn & Bacon 2004* diHM.4 El a-T-M< "Copyright (C) Allyn& Bacon 2124 一 Wisconsin Card Sorting Test125Copyr