Affymetrix基因芯片产品

1、AFFYMETRIX基因芯片产品*芯片产品分类表达谱芯片-mRNA3IVTWTMicroRNA芯片lncRNA芯片SNP/CNV芯片甲基化&转录因子芯片表达谱芯片3IVTHuman U133 Plus 2.0Mouse U430 2.0Rat U230 2.0PrimeView&Almac XcelWTHuman/Rat/Mouse Gene1.0 STHuman/Rat/Mouse Exon1.0 STHTA2.0表达谱芯片探针设计Exon 1.0 ST基因组DNA初始转录本RNA可变剪切转录本Gene 1.0 ST3端表达谱表达谱芯片实验步骤样本RNA抽提&质检cD

2、NA合成及片段化生物素标记芯片杂交-洗脱-扫描芯片数据标准化GENE系列芯片Transcript coverage of the arrayHuman Gene 1.0 ST ArrayHuman Gene 2.0 ST ArrayTotal RefSeq transcripts covered36,07940,716NM ? RefSeq coding transcript, well-established annotation32,02030,654NR ? RefSeq non-coding transcript, well established annotation2,9675,6

3、38XM ? RefSeq coding transcript, provisional annotation579996XR ? RefSeq non- coding transcript, provisional annotation5133,428lincRNA transcripts46611,086RefSeq (Entrez) gene count21,01424,838EXON系列芯片Design Statistics SummaryProbe Sets1.4 millionExon Clusters 1 millionSupported by Putative Full-Len

4、gth mRNA289,961 probe setsSupported by Ensembl Transcripts306,583 probe setsSupported by EST665,175 probe setsSupported by Mouse or Rat mRNA220,262 probe setsSupported by Gene Prediction883,105 probe setsProbe Selection RegionAlong the entire length of the transcriptsProbes/Probe Selection Region4Ba

5、ckground Subtraction StrategyMedian intensity of up to 1,000 background probes with the same GC contentTotal Features Per Array5,500,000Interrogated StrandSenseHTA2Array contentProtein coding contentNon-protein coding contentGenes (transcript clusters)44,69922,829Transcripts245,34940,914Exons560,472

6、109,930Exon clusters296,05882,444Splice junctions (probe sets)339,1460表达谱芯片应用U133经典，相对旧，无价格优势，不做推荐Gene系列基本，仅满足一般表达量检测需求Exon系列全面，精确表达量检测HTA2最佳，最全&最精确表达量EG1.0最佳，唯一能够获得转录本表达量MICRORNA芯片MicroRNA 4.0microRNAPre-microRNAsnoRNA&scaRNAMICRORNA芯片实验步骤样本RNA抽提&质检Poly (A) 加尾&FlashTag Biotin HSR 连接

7、生物素标记芯片杂交-洗脱-扫描芯片数据标准化MICRORNA4.0特点100%覆盖miRBase V20包括所有数据库记录的物种人 2578小鼠 1908大鼠 728重复性0.95技术重复生物重复LNCRNA芯片Human/Mouse/Rat Gene2.0HTA2.0(Human)EG1.0-Human/MouseLNCRNA芯片实验流程样本RNA抽提&质检cDNA合成及片段化生物素标记芯片杂交-洗脱-扫描芯片数据标准化SNP/CNV芯片SNP6.0CytoScan 750KCytoScan HDOncoScan订制芯片CYTO芯片适用样本细胞组织血液唾液骨CYTO HD 270万CNV探针 75万SNP探针高覆盖率 特异性 灵敏度高性能 1 marker/1-3KB 1marker/5KB高分辨率36000120005005000500010000150002000025000300003500040000RefseqOMIMSangerISCA基因数量CYTO系列芯片价值杂合性缺失单亲二倍体三倍体样本异质性家系分析甲基化芯片 Human Promoter1.0 RDNA甲基化CpG岛在CpG甲基化结合蛋白和DNA甲基化转移酶的作用下，使CpG二核苷酸5端的胞嘧啶转变成为5甲基胞嘧啶。通常是基因的启动子区域，5端非翻译区和第一个外显子区，CpG序列密