ABL800血气分析仪资料：01－2定标

1、1 Calibration 定标定标 2 什么是定标(calibration)? Liquid or gas with a known parameter value is drawn into the measuring chamber 将已知数值的液体或气体引入测量室 The electrode measures an electrical signal corresponding to the known value of the liquid or gas 电极测量出一对应的电信号值 This is repeated with liquid or gas of another know

2、n parameter value 用另一个已知数值的液体或气体重复上述过程 The analyzer establishes a calibration line for each parameter and adjusts its readings to measure the known value of each parameter 仪器建立了一条定标曲线,将读数调到相应已知值(每个测量参数都有一条) 在进行测量之前在进行测量之前, ,首先要用标准的液体及气体来确定电极的工作曲线首先要用标准的液体及气体来确定电极的工作曲线 通常把确定电极系统工作曲线的过程叫做定标或校准通常把确定电极系

3、统工作曲线的过程叫做定标或校准 3 Purpose of calibrate? 定标的目的? The calibration process determines and checks the accuracy with which Analyzer measures its parameters 定标过程是决定决定和检查检查仪器测量参数的准确度 Which Measuring？哪里测量 Electrical signal（电信号）：Electrode 电极 Readings（读数）：Display and report 显示和报告 4 Calibration line 定标曲线 The ca

4、libration line represents the relationship between the electrical signal measured at the electrode and the concentration of the substrate specific to the electrode 定标曲线表示了电信号值与特定电极所检测参数之浓度的对应关系 6.8007.400 106 69 pH mV 7.4006.800 Example： the pH electrodes Calibration 5 Obtaining a calibration line 获

5、得定标曲线 Each electrode has a different calibration line 每个电极都有一条不同的定标曲线 The electrical signals from two calibration solutions of known pH are plotted （Example Above）由二个已知PH的定标液得到电信号 Example： Cal 1 solution with pH = 7.400 gives a reading of 106 mV Cal 2 solution with pH = 6.800 gives a reading of 69 m

6、V 6.8007.400 106 69 mV pH 6 Obtaining a calibration line 获得定标曲线 The relationship between measured potential and concentration is linear 测量的电压与浓度间的关系成线性 The calibration line forms the scale used to convert the measured potential to pH 作为一标尺，把测量的电压值转变为PH 6.8007.400 106 69 mV pH 7 A 1point calibration

7、line versus a 2point 1点定标与2点定标 Only Cal 1 is measured, giving one calibration point 只测量1个数值，得到一个定标点 A calibration line with the same slope as the previous 2point calibration is superimposed on this new calibration point 在新的1点定标点上与以前的2点定标由相同的斜率 106 69 mV pH 6.8007.400 2P cal. New Cal1 新的新的1点定标值点定标值 8

8、 Sensitivity (slope) 灵敏度（斜率） The sensitivity is an evaluation of the slope of a 2point calibration line in relation to the slope of the theoretical calibration line 灵敏度是用2点定标的斜率与理论上的斜率相关性来评估的 理论上的斜率是由 Nernst 方程得来的 6.8007.400 106 69 mV pH 实际的实际的 理论的理论的 9 Sensitivity, an example 灵敏度举例 During a 2point

9、calibration the following is measured: 7.400 106 69 mV pH 101 65 E pH Theo. 2P-cal. 6.800 - Cal 1 solution with pH = 7.400 gives a reading of 101.0 mV - Cal 2 solution with pH = 6.800 gives a reading of 65.0 mV 10 Sensitivity, an example 灵敏度举例 Pco2灵敏度计算公式: 11 Monitoring the calibration line 监视定标曲线 每

10、一次定标时都校正定标曲线，考虑方面： Sensitivity Status/zero point 灵敏度状态、零点 Describes the slope and position of the calibration Line 描述斜率和定标曲线位置 Drift 漂移 Describes the variation in the calibration line between consecutive Calibrations 描述连续定标间定标曲线的变化 每个电极都有它自己的状态和灵敏度限度 12 Example：sensitivity limits 灵敏度限度 E pH Theo. 2P

11、-cal. E pH Theo. 2P-cal. % For a calibration to be accepted, the sensitivity must fall within the following limits 必须落在限度内，定标才可以 被接受（Calibration OK!） 13 Status 状态 The status describes the deviation of a 1point calibration point from the theoretical calibration line 状态描述了1点定标与理论定标曲线的偏离 the status is

12、evaluated using only one calibration point; i.e. we assume that the sensitivity (slope) is unchanged 状态只能从1点定标点评估，因为我们假设斜率不变的 6.8007.400 pH 69 Theo. 1P-cal. mV 106 100 E 7.300 14 Example： status limits 状态限度 For a calibration to be accepted, the status and zero point must fall within the following li

13、mits 状态和零点必须落在限度内定标才能被接受（Calibration Passed） Theo. cal. Theo. cal. % ParameterStatus limits pH6.78.1 pCO26.2260 mmHg cK+0.512 mmol/L cNa+10250 mmol/L cCl 30900 mmol/L cCa2+0.120 mmol/L Zero point pO2 6 mmHg (0.8 kPa) cGlu 10000 pA cLac 10000 pA 15 Drift 漂移 The drift of an electrode is a description

14、of how much a calibration differs from the last calibration 电极漂移的描述：这次定标与最近一次定标的差值电极漂移的描述：这次定标与最近一次定标的差值 Drift is a check of whether the adjustment performed during calibration is acceptable 定标已接受时，漂移是检查运行是否需要调整定标已接受时，漂移是检查运行是否需要调整 This is defined by the Drift Tolerances 有漂移限度的定义有漂移限度的定义 16 漂移的计算 6.

15、800 7.400 mV pH 第一次第一次 2P-cal 斜率斜率 第一次第一次 2P-cal 第二次第二次 2P-cal. Drift 1 Drift 2 17 Drift tolerances 漂移限度 Drift values lying outside the tolerance limits indicate that Theelectrode is unstable and thus incapable of providing Reliable Calibrations 漂移值落在限度范围之外，表示：漂移值落在限度范围之外，表示： 电极不能用了，不能进行有效的可靠的定标电极不能用了，不能进行有效的可靠的定标 18 Sensitivity of pO2 electrode 氧电极的灵敏度 从气体1和气体2（ Gas 1 & Gas 2 )得到2点定标值 0.0 60 pA kPa 2P-cal. 18.6 2800 19 pO2 zero point 氧零点 Calculated from the sensitivity and the current measured at pO2 = 0 0.018.6 60 2800 kPa 第一次第一次 2P