第15章 志贺菌属

1、Medical Microbiology,Department of Microbiology, HMU,第二篇,致病性细菌,第,15,章,志贺菌属,Shigella,微生物学教研室,张文莉,Department of Microbiology, Harbin Medical University,教学大纲,?,熟悉,?,志贺菌属生物学性状特点（抗原构造及分类）,?,志贺菌的致病物质及所致疾病,?,志贺菌感染的微生物学检查法,?,掌握,?,志贺菌的防治原则,Department of Microbiology, Harbin Medical University,问题,?,志贺菌属的致病物质及

2、其作用机制是什么？,?,对可疑痢疾患者如何进行微生物学检查？,Department of Microbiology, Harbin Medical University,生物学性状,?,形态染色,?,G,-,杆菌，,0.5-0.7 ,m,?,2-3,m,?,无鞭毛，无荚膜，,有菌毛,Department of Microbiology, Harbin Medical University,生物学性状,?,培养及生化反应,?,营养要求不高,?,不分解乳糖,?,ss,选择培养基,?,克氏双糖管,Department of Microbiology, Harbin Medical Universit

3、y,抗原构造及分类,?,由,O,和,K,抗原组成,?,分类：,菌种,群,型,亚型,?,痢疾志贺菌,A 1,10 8a,、,8b,、,8c,?,福氏志贺菌,B 1,6,、,x 1a,、,1b,、,2a,、,2b,y,变型,3a,、,3b,、,3c,、,4a,、,4b,?,鲍氏志贺菌,C 1,18,?,宋内志贺菌,D 1,Department of Microbiology, Harbin Medical University,?,比其他肠道杆菌弱,?,对酸和一般消毒剂敏感,抵抗力,Department of Microbiology, Harbin Medical University,致病物质

4、,?,侵袭力,?,内毒素,?,外毒素,Department of Microbiology, Harbin Medical University,所致疾病,?,菌痢,?,患者和带菌者,?,粪,-,口,?,表现：,?,潜伏期数小时至,7d,?,发热、腹痛、腹,泻、里急后重、,粘液脓血便,Department of Microbiology, Harbin Medical University,痢疾患儿,Department of Microbiology, Harbin Medical University,免疫性,?,免疫期短，不稳固,?,免疫主要是消化道粘膜的,SIgA,Department of Microbiology, Harbin Medical University,微生物学检查法,?,标本,?,分离培养与鉴定,?,毒力试验,?,快速诊断,?,免疫荧光菌球法,?,协同凝集试验,?,胶乳凝集试验,?,PCR,、杂交,Department of Microbiology, Harbin Medical University,防治原则,?,隔离患者、彻底治疗,?,试用的口服菌苗,?,抗生素,Department of Microbiology, Harbin Med