常用酶切位点表含保护碱基

1、 切割率%寡核苷酸序2h20hGTCGAAccCGTCGACCCGTCGACGACATGAflIIC9ACATG9GCC9ACATGGG9GGCGCGC99Asc99GGCGCGCTGGCGCGCA9959CCCGGAvaC99CCCGGGTCCCCGG99GG2GGATC1BamHCGGATC99CCG9GGATCGC9AGATCBglIG9AGATC7TGGAGATC9TC2GCGCGBssHIAGCGCGCTT59GCGCGCAGGT(A/T)AC1BstEIAACTGCAGACCAATGCATTGBstXAAAACTGCA52CCAATGCATTGACTGCAGA29CCAATGCAT

2、TGATGCAATCGAClaATCGAC9ATCGA9GCCATCGA55GG99GAATTEcoRC99GAATTCCCGAATT9CG9GGGCC99HaeIIAGGGC99GC TTGCGGCCGCAA 90 90 HindIIICAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 KpnIGGGTACCC GGGGTACCCC CGGGGTACCCCG 0 90 90 0 90 90 MluIGACGCGTC CGACGCGTCG 0 25 0 50 NcoICCCATGGG CATGCCATGGCATG 0 50 0 75 NdeICCATA

3、TGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 90 90 NheIGGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 酶 寡核苷酸序列 切割率% 2hr 20hr NotITTGCGGCCGCAA TTTAATTTGCGGCCGC AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT GCGGCCGCAAGGAAAAAAAAAAGGA

4、AAA 0 10 10 25 25 0 10 10 90 90 NsiITGCATGCATGCA CCAATGCATTGGTTCTGCAGTT 10 90 90 90 PacITTAATTAA GTTAATTAAC CCTTAATTAAGG 0 0 0 0 25 90 PmeIGTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG 0 0 0 75 0 25 50 90 PstIGCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA AACCAAT

5、GCATTGGATGCATCTGCAG 0 10 90 90 0 0 10 90 90 0 PvuICCGATCGG ATCGATCGAT TCGCGATCGCGA 0 10 0 0 25 10 SacICGAGCTCG 10 10 SacIIGCCGCGGC TCCCCGCGGGGA 0 50 0 90 SalIGTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA 0 10 10 0 50 75 ScaIGAGTACTC AAAAGTACTTTT 10 75 2

6、5 75 SmaICCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 0 0 10 90 10 10 50 90 SpeIGACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG 10 10 0 0 90 90 50 50 SphIGGCATGCC CATGCATGCATG ACATGCATGCATGT 0 0 10 0 25 50 StuIAAGGCCTT GAAGGCCTTC AAAAGGCCTTTT 90 90 90 90 90 90 XbaICTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG 0 90 75 75 0 90 90 90 XhoI CCTCGAGG GGCCCTCGAG CGGCC