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高效薄层色谱法鉴别6种中药多糖杨成，管佳，章江生，李绍平A1A2A0A3A4A5A6A7A8A9A10A11A13A2A0A14A12A15A15A16A16A17A18A19A20A21A22A23A24A25A26A27A28A29A30A31A32A33A34A35A36A37A38A39A40A41A42A43A44A45A46A47A48A49A50A51A52A53A54A552A56A57A58A59A60A61A62A63A64A65A66A67A51A68A69A70A71A72A73A74A44A45A75A76A77A78A79A80A81A82A83A84A85A86A87A88A86A87A89A90A87A91A92A93A87A941255451515V/VA95A96A97A98A997A100A101A102A103A104A1052A100A104A106A107A95A108A109A99A110A111A112A113A114A112A113A95A104A115A116A117A118A119A98A99A120A121A122A123A124A118A119A98A125A126A127A128A107A115A116A117A99A129A130A131A132A104A107A133A134A135A136A137A138A115A116A117A139A140A141A142A143A119A144A99A145A111A146A147A117A148A149A150A151A152A153A154A155A156A154A157A158A154A159A160A154A161A162A160A105A123A163A1396A100A132A104A164A117A165A166A167A166A167A166A168A169A131A170A100A145A171A1726A100A137A173A132A104A139A174A175A142A143A119A144A176A99A177A176A178A103A179A180A99A181A182A183A111A99A145A184A111A146A132A104A115A116A117A185A186A187A188A165A189A190A191A192A132A104A99A174A175A142A143A119A144A99A185A186A187A188A99A137A173DISCRIMINATIONOFPOLYSACCHARIDESFROMSIXTRADITIONALCHINESEMEDICINESUSINGHIGHPERFORMANCETHINLAYERCHROMATOGRAPHYYANGCHENG,GUANJIA,ZHANGJIANGSHENG,LISHAOPINGINSTITUTEOFCHINESEMEDICALSCIENCES,UNIVERSITYOFMACAU,MACAOSAR,CHINAABSTRACTOBJECTIVETODISTINGUISHTHEPOLYSACCHARIDESFROMDIFFERENTTRADITIONALCHINESEMEDICINESTCMSMETHODSTHEACIDHYDROLYZATESOFPOLYSACCHARIDESWEREANALYZEDBYHIGHPERFORMANCETHINLAYERCHROMATOGRAPHYHPTLCCOMBINEDWITHTWOCOLORATIONMETHODSANDTHINLAYERSCANNINGTECHNIQUERESULTSTHECHROMATOGRAPHYWASPERFORMEDONNANOSILICAGEL60PLATEWITHNBUTANOLMETHANOLCHLOROFORMACETICACIDWATER1255451515,V/V/V/V/VASMOBILEPHASE7MONOSACCHARIDESAND2GLYCURONICACIDSWEREUSEDASREFERENCECOMPOUNDSTHEANILINEDIPHENYLAMINESOLUTIONANDNINHYDRINSOLUTIONWEREEMPLOYEDFORDETECTIONOFSACCHARIDESANDAMINOACIDS,RESPECTIVELYTHEPOLYSACCHARIDESFROMCORDYCEPSSINENSIS,GANODERMALUCIDUM,ASTRAGALUSMEMBERANACEUS,PANAXGINSENG,PANAXQUINQUEFOLIIANDPANAXNOTOGISENGWEREEASILYDISCRIMINATEDBASEDONTHEIRCHARACTERISTICTLCPROFILESCONCLUSIONASIMPLE,RAPIDANDEFFECTIVEHPTLCMETHODWASDEVELOPEDFORDISTINGUISHINGTHEPOLYSACCHARIDESFROM6TCMS,WHICHISHELPFULTOCONTROLTHEQUALITYOFPOLYSACCHARIDESFROMCHINESEMEDICINEKEYWORDSPOLYSACCHARIDESHPTLCQUALITYCONTROLTCMSA193A194A195A196A197A198A199A194A200A201A202A203A20410A205A206A201A207A194A208A209A210A211A212A213A214A215A216A203A217A218A219A220A216A221A195A215A216A222A223A224A215A225A226A227A214A228A229A216A230A231A232A233A234A235A236A237A238A239A240A241A242A243A240A244A239A245A246A247A248A249A250A251A252A253A245A235A254A255A150A0A249A129A244A136A1A235A153A154A2A3A235A157A4A5A6A235A154A7A8A914A164A253A10A250A251A11A12A249A13A14A15A243A145A235A153A169A16A17A18A19A234A20A249A239A240A250A251A103A21A22A23A24A25A235A173A22A250A251A26A27A28A29A249A30A31A175A25A15A176A32A250A33A34A35A36A181A245A37A38A39A235A153A40A41A42A435A90A183A44A42A436A90A45A46A47A44A42A437A90A26A438A90A186A48A49A509A9A164A163A51A52A250A251A249A53A54A245A37A239A235A255A239A40A41A42A43A254A255A148A55A90A106A56A90A191A57A27A58A90A253A57A72A9A59A14A164A253A10A55A251A90A195A251A253A245A16A17A249A60A51A38A395A15A57A196A163A51A45A46A40A41A42A43A117HPTLCA122A39A656A33A60A51A239A240A250A251A249A61A62A63A64A244A66A67A245A37A235A687A33A55A251A692A33A251A70A61A10A65A71A235A73A512A33A18A19A249A74A42A75A65A100A253A251A252A253A245A241A53A76A80A77A61A252A253A245A245A17A74A42A235A78A247A79A81A51A52A16A17A249A82A83A40A41A42A43A151A84A85A86A87A88A84A85A86A87DESGAA89A91A92A93A199A94A95A200AS30A96A97A98A99A101A102CD60A104A105A107A102PROQUANTA104A105A108A109A203A110DESGAA205A111A112A113A208A209A114A115A116A113A118A119A120A12160A213A123A124A125MACHEREYNAGELA126A127A112A128A214A130A131A217A132MULTIWAVE3000,ANTONPAAR,A218A133A134DA221A135A137A138DA223A139A137A138DA140A137A138DA225A137A138DA226A141A137A138LA228A142A143A137A138LA231A144A137A138DA221A135A137A146A147A138DA223A139A137A146A147A233A125SIGMAA126A127A149A123MILLIQA234A149A112A113A151A152A155A156A158A159A160A161A234A128A162A165A123PANAXGINSENGA112A138A166A167A165A123PANAXQUINQUEFOLIIA112A138A168A170A123PANAXNOTOGINSENGA112A138A171A172A174A177A123CORDYCEPSSINESISA112A238A178A239A179A180A182A165A184A126A127A138A185A187A123ASTRAGALUSMEMBERANACEUSA112A188A189A243A166A138A190A192A123GANODERMALUCIDUMA112A188A189A244A193A194A197A138A158A237A198A201A202A204A206A207A1282A210A211A210A21121A212A215A216A219A220A222A224A227A215A216A219A220A224A227A229DA230A232A23505MGA236DA240A23515MGA236DA241A23515MGA236DA242A245A23505MGA236LA246A247A248A23510MGA236LA249A250A23505MGA236DA251A252A235A253A25410MGA236DA230A232A235A253A25410MGA236A255A252A254A0A29A1A841ML95A2A50A3A4A5A74A6A7A8A1A185A1222A26A9A21A10A11A13A14A15A10A11A14A15A217A16A17A18A19A20A221GA23A24A2510MLA27A23A28A214A30A31A32A33A34A35120CA36A31A3215MINA37A31A32A216A354500RPMA36A146A3810MINA39A32A40A41A216A37A42A4340ML95A44A45A39A354CA55A46A47A45A48A123A49A51A52A53A216A54A56A146A38A37A57A58A40A41A216A37A48A59A605ML95A44A45A61A622A56A39A63A64A65A66A67A68A69A70A51A32A68A69A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