大鼠下丘脑室旁核内一氧化氮合酶神经元的生后发育.doc

大鼠下丘脑室旁核内一氧化氮合酶神经元的生后发育解剖科学进展 2000年第3期第6卷 原著作者：王兰 宋天保 周劲松单位：王兰（陕西省高等医学专科学校解剖教研窒 西安 710068）；宋天保 周劲松（西安医科大学组织与胚胎学教研室）关键词：一氧化氮合酶；视上核；室旁核；生后发育；NADPH-黄递酶组织化学；免疫组织化学；大鼠摘要：应用NADPH-黄递酶组织化学和一氧化氮合酶(NOS)免疫组织化学方法以及计算机图像分析，观察NOS神经元在大鼠下丘脑室旁核(PVN)生后发育各阶段的形态及分布特征。结果显示，1 d时PVN内已有NOS神经元。随着生长发育，PVN的面积逐渐变化，NOS神经元主要集中在PVN的外侧大细胞部及腹侧部，细胞逐渐增多，胞体的平均截面积逐渐变大，平均灰度值逐渐降低。14 d以后至成年鼠(90 d)以上变化不明显。结果提示，PVN中NOS神经元的生后发育和成熟主要在14 d前，尤以7 d至14 d为关键时期。Postnatal Development of NOS Neurons in the Paraventricular Nucleus of Rat Hypothalamus wang LanAbstract：In the present research, the postnatal development of nitric oxide synthase(NOS) neurons in the hypothalamic paraventricular nucleus (PVN) of the rat was studied by means of nADPH-d histochemical, NOS immunohistochemical methods and image analysis. The results showed that NOS neurons were present in PVN on day 1 of postnatal life. With the development, the PVN was gradually enlarged in size and NOS neurons were predominantly confined within lateral magnocellular and ventral subdivisions of pVN. NOS neurons were increased in number and the soma area was increased while grey level decreased. The number and morphology of NOS neurons remained unchanged after day 14. These results indicate that the main developmental stage of NOS neurons is from d 1 to d 14, especially from d 7 to d 14.Keywords：nitric oxide synthase; paraventricular nucleus; postnatal development; NADPH-d histochemical; immunohistochemical; rat参考文献：1钟慈声，孙安阳一氧化氮的生物医学上海上海医科大学出版社，1997; 162Hope BT, Micheal GJ, Kingge KM, et al. Neuronal NADPHdiaphorase is a nitric oxide synthase. Proc Nad Acad SCi USA, 1991, 88: 28113Areralo R, Sanchez F, Alonso JR, et al. NADPH-diaphorase activity in the hypothalamic magnocellular, neurosecretory nuclei of the rat. Brain Res Bull, 1992, 28: 5994Aimi Y, Fujimura M, Vincent SR, et al. Localization of NADPH-diaphorase-containing neurons in sensory gangem of the rat. J Comp neurol, 1991, 306: 3825Vahschanoff JG, Weinberg RJ, Rustioni A. NADPH-diaphorase in the spinal cord of rats. J Comp neurol, 1992, 321: 2O96贾铀生，鞠躬神经系统中的一氧化氮合酶神经解剖学杂志，1993，9: 17王百忍，赵经纬一氧化氮合酶与痛神经解剖学杂志，1995，11: 698倪衡建，凌树才，万人欣，等大鼠下丘脑室旁核中一氧化氮合酶阳性神经元的生后发育神经解剖学杂志，1997，13(2): 1719朱长庚，张喜京，刘汉涛，等室旁核胆囊收缩素神经元的电镜免疫细胞化学研究解剖学报，1990，21: 24810Okamura H, Yokosuka M, Hayashi S. Estrogenic induction of NADPH-diaphorase activity in the prcoptic neurons containing estrogen recepto