




版权说明:本文档由用户提供并上传,收益归属内容提供方,若内容存在侵权,请进行举报或认领
文档简介
1、Chapter 7,Chromosomes, chromatin, and the nucleosome,Nucleus: 细胞核; Nucleolus: 核仁 Nucleoid: 类核 Mitosis: 有丝分裂;Meiosis:减数分裂 interphase:分裂间期 Histone: 组蛋白;Nucleosome: 核小体 Chromosome: 染色体; Chromatin: 染色质;eu-; hetero- Centromere (中心粒) Telomere(端粒) Repetitive DNA (重复DNA) Tandem gene cluster(串联基因 簇),Vocabula
2、ry,Characteristics of Eukaryotic Genome,The importance of packing of DNA into chromosomes,Chromosome is a compact form of the DNA that readily fits inside the cell To protect DNA from damage DNA in a chromosome can be transmitted efficiently to both daughter cells during cell division Chromosome con
3、fers an overall organization to each molecule of DNA, which facilitates gene expression as well as recombination,Chromosome The nucleosome Higher-order chromatin structure Regulation of chromatin structure Nucleosome assembly,Chromosomes: DNA,Shape: circular or linear Number in an organism is charac
4、teristic Copy: haploid, diploid, polyploid,problem,Genome size: the length of DNA associated with one haploid complement of chromosomes Gene number: the number of genes included in a genome Gene density: the average number of genes per Mb of genomic DNA,Genome presence of introns (splicing),unique;
5、repeated,2.Increases in the DNA between genes (intergenic sequences):,Pseudogenes are inactive but stable components of the genome derived by mutation of an ancestral active gene.,Pseudogene,the formation of pseudogene,Figure 6.8 the formation of truncated gene(截短基因) and gene fragment.,tandem repeti
6、tive DNA Satellite DNA: Minisatellite DNA Microsatellite DNA Dispersed repetitive DNA 产生原因转座: 遗传元件在DNA中从一个位置移动到另一个位置的过程 RNA介导真核存在/原核无 机制: Retrotransposition (反转录转座) 反转录元件 DNA转座子原核常见/真核少 机制:复制型和保守型 水平基因转移,3. The Repetitive DNA content of Genomes (重复DNA序列),Figure6.15 Satellite DNA from the human genom
7、e,Satellite DNA (卫星DNA): 核心序列为5200bp ,主要存在于染色体着丝粒区,,Centrifugation(离心分离技术),常用的两类离心分离方法是 差速离心(differential centrifugation) 密度梯度离心(density gradient centrifugation),离心分离细胞组分和生物分子是最常用的分离方法,蔗糖密度梯度离心分离溶酶体、线粒体和微粒体,CsCl 密度梯度离心分离DNA,Figure6.15 Satellite DNA from the human genome,Satellite DNA (卫星DNA): 核心序列为5
8、200bp ,主要存在于染色体着丝粒区,,Minisatellite DNA(小卫星DNA)核心序列为25bp ,主要存在于染色体靠近端粒处,与DNA复制有关染色体其他位置的小卫星DNA,功能不清,DNA复制时微卫星拷贝有时发生滑移,导致插入或缺失一个或多个重复单位,使核心序列重复数目发生变化,以至于任何两个不同个体间中都存在串联数目的差异-可用于DNA Finger(DNA 指纹) 可用来研究人群相关性,Microsatellite DNA (微卫星DNA),指以27个碱基为核心单位串联重复而成的一类序列,又称为STR short tandem repeat(短串联重复序列),Microsa
9、tellites are useful for genetic mapping,Origins of replication Centromeres Telomeres,Critical DNA elements in Chromosome duplication & segregation,Figure 7-6 Centromeres, origin of replication and telomere are required for eukaryotic chrom. maintenance,Mitotic chromosome at metaphase,Kinetochore 动粒,
10、Centromere 着丝粒,Centromere: 着丝粒, a region of chromosome (染色体) where two sister chromatids(染色单体)are joined at metaphase. 2. Kinetochore: 动粒, a protein complex attaching microtubules(微管) of mitotic spindle (纺锤体) 3. Telomere: 端粒, end of chromosome,Mitotic spindle,Telomere 端粒,Direct the formation of kine
11、tochore (an elaborate protein complex) essential for chromosome segregation One chromosome, one centromere The size varies (200 bp- 40 kb) Composed of largely repetitive DNA sequences,Centromeres,Required for the correct segregation of the chromosomes after replication,Chromosome,The majority of the
12、 associated proteins are small, basic proteins called histones. Other proteins associated with the chromosome are referred to as non-histone proteins, including numerous DNA binding proteins that regulate the transcription, replication, repair and recombination of DNA.,Half of the molecular mass of
13、eukaryotic chromosome is protein,Chromosome,The nucleosome,Discovery Components Atomic structure,Nucleosomes,1973-1974: Nuclease protection analysis,2. 1974: electron micrographs,3. Biochemistry analysis: Component: eight histone proteins (H2A,H2B,H3,H4) + 140150bp DNA,Nuclease protein analysis of c
14、hromatin from hunman nulei,The nucleosome components,DNA Proteins,core DNA: 147 bp linker DNA: 20-60 bp,Histones,Five abundant histones Structural fold N-terminal “tail”,Histones are small, positively charged (basic) proteins,Figure 7-19 The core histones share a common structural fold,histone-fold
15、domain,the sites of extensive modifications,H2B,H2A,H3,H4,a2,a3,a1,a2,a3,a1,a2,a3,a1,a2,a3,a1,L1,L2,aN,H3,H4,4 helix-bundle,H3,H4,H3,H4,4 helix-bundle,H4,H2B,H2A,H4,H2B,H2A,H3,H4,H2B,H2A,H3,Diameter = 110 A,H4,H2B,H2A,H3,Width = 45 A,Chromosome,The nucleosome,Discovery Components Atomic structure,Hy
16、drogen bonds: histone (140) DNA: phosphodiester backbone 7 : Bases,1.sequence-independent contacts,2. The histone N-terminal tails,Minor groove,The histone tails emerge from the core of the nucleosome at specific positions, serving as the grooves of to direct the DNA wrapping around the histone core
17、 in a left-handed manner.,a screw,Chromosome The nucleosome,Higher-order chromatin structure,Histone H1 binds to the linker DNA 30-nm fiber Further compaction of DNA Histone variants,Histone H1 binds to the linker DNA,H1 binds two distinct regions of DNA,one end of the nucleosome central DNA helix o
18、f the nucleosome,The addition of Histone H1 lead to more compact nucleosomal DNA,the 30-nm fiber,6 nuleosomes/superhelix Linker DNA circles around the central axis,Linker DNA pass through the central axis,Further compaction of DNA involves large loops of nucleosomal DNA,Additional 103-104-fold compa
19、ction is required, but the mechanism is unclear The nuclear scaffold model is proposed,The higher-order structure of chromotin,Histone variants,Several histone variants are found in eukaryotes This variants can replace one of the 4 standard histones to form alternate nucleosomes,H2A.Z,CENP-A is asso
20、ciated with the nucleosomes containing centromeric DNA,Figures 7-33 Alteration of chromatin by incorporation of histone variants,H3,Chromosome The nucleosome Higher-order chromatin structure,Regulation of chromatin structure,Nucleosome positioning Modification of the N-terminal tails of the histones
21、,DNA Must Be Accessed,The interaction of DNA with the histone octamer is dynamic,Nucleosome remodeling complexes,A large protein complexes facilitate changes in nucleosome location or interaction with the DNA using the energy of ATP hydrolysis.,Fig 7-35 Nucleosome movement catalyzed by nucleosome remodeling complexes,remodling,Nucleosome positioning,Some Nucleosome are found in special position in vivo.,DNA binding proteins Particular
温馨提示
- 1. 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。图纸软件为CAD,CAXA,PROE,UG,SolidWorks等.压缩文件请下载最新的WinRAR软件解压。
- 2. 本站的文档不包含任何第三方提供的附件图纸等,如果需要附件,请联系上传者。文件的所有权益归上传用户所有。
- 3. 本站RAR压缩包中若带图纸,网页内容里面会有图纸预览,若没有图纸预览就没有图纸。
- 4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
- 5. 人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对用户上传分享的文档内容本身不做任何修改或编辑,并不能对任何下载内容负责。
- 6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
- 7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。
最新文档
- 2025年中国FM自动选台收音机望远镜数据监测研究报告
- 2025年中国BOPP镀铝膜数据监测研究报告
- 2025年中国2、5-二甲基苯胺数据监测报告
- 2025至2030年中国集中操作式电梯井筒模市场分析及竞争策略研究报告
- 2025至2030年中国铝合金吸顶灯市场分析及竞争策略研究报告
- 2025至2030年中国茴香粉市场分析及竞争策略研究报告
- 2025至2030年中国砼搅拌站市场分析及竞争策略研究报告
- 2025至2030年中国电力控制开关柜市场分析及竞争策略研究报告
- 2025至2030年中国深沟外球面球轴承市场分析及竞争策略研究报告
- 2025至2030年中国汽车储液器支架市场分析及竞争策略研究报告
- 初三励志主题班会:初三第一次班会幻灯片PPT
- GB/T 42910-2023无机胶粘剂高温压缩剪切强度试验方法
- 2022年江苏省射阳中等专业学校招聘考试真题及答案
- 给搅拌站送石子合同范本
- 2023年副主任医师(副高)-学校卫生与儿少卫生(副高)考试历年真题集锦带答案
- 法律基础(第4版)PPT完整全套教学课件
- 仓管应聘求职简历表格
- 五年级下册语文期末考试学霸夺冠解密卷人教部编版含答案
- 房屋加固工程监理规划
- 一级烟草专卖管理师理论考试题库(含答案)
- von frey丝K值表完整版
评论
0/150
提交评论